##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
ApoI AscI AseI AsnI Asp700I
AspI AviII BbrPI BclI BfrI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BsrGI BssHII Bst1107I BstAPI
BstBI BstZ17I ClaI CpoI DraI
DraIII EagI EclXI Eco52I EcoRI
EcoT22I FbaI FseI FspI HincII
HindII HpaI KspI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XmnI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AccI 874 GTCTAC GT^[AC][GT]AC
AccIII 3743 TCCGGA T^CCGGA
AhdI 529 GACAACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 529 GACAACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4199 CCTAGG C^CTAGG
BbeI 1717 GGCGCC GGCGC^C
BlnI 4199 CCTAGG C^CTAGG
BsaAI 3550 TACGTG [CT]AC^GT[GA]
BsaBI 1834 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3743 TCCGGA [AT]^CCGG[AT]
BseAI 3743 TCCGGA T^CCGGA
BspEI 3743 TCCGGA T^CCGGA
BspLU11I 767 ACATGT A^CATGT
BssSI 122 CTCGTG CACGAG(-5/-1)
Eam1105I 529 GACAACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1633 GATATC GAT^ATC
HindIII 616 AAGCTT A^AGCTT
KasI 1717 GGCGCC G^GCGCC
MamI 1834 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3743 TCCGGA T^CCGGA
NarI 1717 GGCGCC GG^CGCC
NspI 767 ACATGT [GA]CATG^[CT]
SfoI 1717 GGCGCC GGC^GCC
Tsp509I 4111 AATT ^AATT
XbaI 364 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 1513 GTGCAC G^TGCAC
3730 GTGCAC
Aor51HI 2232 AGCGCT AGC^GCT
2699 AGCGCT
ApaLI 1513 GTGCAC G^TGCAC
3730 GTGCAC
BbsI 973 GTCTTC GAAGAC(2/6)
1069 GTCTTC
BcgI 2674 CGACACCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3684 GCAGCAGCCTCG
BciVI 831 GGATAC GTATCC(6/5)
1235 GGATAC
BglII 81 AGATCT A^GATCT
1823 AGATCT
BpuAI 973 GTCTTC GAAGAC(2/6)
1069 GTCTTC
BsrFI 278 ACCGGC [GA]^CCGG[CT]
1661 ACCGGC
BstEII 2922 GGTGACC G^GT[ACGT]ACC
3817 GGTCACC
BstPI 2922 GGTGACC G^GT[ACGT]ACC
3817 GGTCACC
BstXI 1743 CCACAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3807 CCAAGATCCTGG
Cfr10I 278 ACCGGC [GA]^CCGG[CT]
1661 ACCGGC
DrdI 3592 GACCAGCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4518 GACCATGGAGTC
Eco47III 2232 AGCGCT AGC^GCT
2699 AGCGCT
EcoO65I 2922 GGTGACC G^GT[ACGT]ACC
3817 GGTCACC
MseI 3545 TTAA T^TAA
4626 TTAA
PaeR7I 2852 CTCGAG C^TCGAG
3105 CTCGAG
SapI 2548 GAAGAGC GCTCTTC(1/4)
2606 GCTCTTC
SmaI 569 CCCGGG CCC^GGG
4471 CCCGGG
StuI 223 AGGCCT AGG^CCT
1038 AGGCCT
Tru9I 3545 TTAA T^TAA
4626 TTAA
XhoI 2852 CTCGAG C^TCGAG
3105 CTCGAG
XmaCI 569 CCCGGG C^CCGGG
4471 CCCGGG
XmaI 569 CCCGGG C^CCGGG
4471 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI Asp718I AspHI AvaI AvaII
BalI BamHI BanI BanII BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII