##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI Asp718I
AviII BanI BbeI BcgI BfrI
BsaBI BsiEI BsiWI BsmI Bsp1407I
BspDI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HindIII KasI KpnI KspI MamI
MfeI MluI MunI NaeI NarI
NgoMIV NotI NruI NsiI NspV
PacI PinAI PmeI Psp1406I PvuI
RsrII SacII ScaI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccII 2010 CGCG CG^CG
AccIII 39 TCCGGA T^CCGGA
AcsI 1234 AAATTT [GA]^AATT[CT]
AcyI 2108 GGCGTC G[GA]^CG[CT]C
ApoI 1234 AAATTT [GA]^AATT[CT]
AseI 4085 ATTAAT AT^TAAT
AsnI 4085 ATTAAT AT^TAAT
BalI 5077 TGGCCA TGG^CCA
BbrPI 5030 CACGTG CAC^GTG
BciVI 2341 GTATCC GTATCC(6/5)
BclI 787 TGATCA T^GATCA
BsaAI 5030 CACGTG [CT]AC^GT[GA]
BsaHI 2108 GGCGTC G[GA]^CG[CT]C
BsaWI 39 TCCGGA [AT]^CCGG[AT]
BseAI 39 TCCGGA T^CCGGA
BspEI 39 TCCGGA T^CCGGA
BspHI 1034 TCATGA T^CATGA
BspLU11I 4108 ACATGT A^CATGT
BspMI 4629 ACCTGC ACCTGC(4/8)
BssSI 374 CTCGTG CACGAG(-5/-1)
BstUI 2010 CGCG CG^CG
FbaI 787 TGATCA T^GATCA
Hin1I 2108 GGCGTC G[GA]^CG[CT]C
HpaI 1661 GTTAAC GTT^AAC
MluNI 5077 TGGCCA TGG^CCA
MroI 39 TCCGGA T^CCGGA
MscI 5077 TGGCCA TGG^CCA
MvnI 2010 CGCG CG^CG
NdeI 1380 CATATG CA^TATG
NheI 2697 GCTAGC G^CTAGC
PaeR7I 3106 CTCGAG C^TCGAG
PmaCI 5030 CACGTG CAC^GTG
PmlI 5030 CACGTG CAC^GTG
PshBI 4085 ATTAAT AT^TAAT
RcaI 1034 TCATGA T^CATGA
SalI 2588 GTCGAC G^TCGAC
SfiI 5078 GGCCAGACGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
XbaI 1529 TCTAGA T^CTAGA
XhoI 3106 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 2588 GTCGAC GT^[AC][GT]AC
2756 GTCTAC
AhdI 105 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2103 GACCTGGCGTC
Asp700I 2599 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2917 GAAGAACTTC
AspEI 105 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2103 GACCTGGCGTC
AspI 1923 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2075 GACTGTGTC
AvaI 3106 CTCGAG C^[CT]CG[GA]G
4195 CTCGGG
BsmBI 4296 CGTCTC CGTCTC(1/5)
1618 GAGACG
BsoBI 3106 CTCGAG C^[CT]CG[GA]G
4195 CTCGGG
BsrDI 4037 CATTGC GCAATG(2/0)
2701 GCAATG
Eam1105I 105 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2103 GACCTGGCGTC
MslI 262 CACACTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
5030 CACGTGTGTG
PflFI 1923 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2075 GACTGTGTC
SapI 2737 GAAGAGC GCTCTTC(1/4)
3627 GAAGAGC
SbfI 439 CCTGCAGG CCTGCA^GG
4630 CCTGCAGG
Sse8387I 439 CCTGCAGG CCTGCA^GG
4630 CCTGCAGG
StuI 3321 AGGCCT AGG^CCT
4757 AGGCCT
Tth111I 1923 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2075 GACTGTGTC
XcmI 843 CCAATCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3704 CCAGCCTTCACCTGG
XmnI 2599 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2917 GAAGAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AocI ApaI AspHI AvaII
AvrII BamHI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrBI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PshAI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII