##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AatII       AclI        AflII       AgeI        AhdI        
AscI        AseI        AsnI        AspEI       AvrII       
BciVI       BclI        BfrI        BlnI        BlpI        
Bpu1102I    BsaBI       BsiEI       BsiWI       Bsp1407I    
BspDI       BspHI       BsrGI       BssHII      BstBI       
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco52I      
EcoRV       EcoT22I     FbaI        FseI        KspI        
MamI        MfeI        MluI        MunI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PinAI       PmeI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SbfI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccIII       3294  TCCGGA               T^CCGGA
AcyI         3925  GGCGCC               G[GA]^CG[CT]C
Alw44I       4777  GTGCAC               G^TGCAC
ApaI         4001  GGGCCC               GGGCC^C
ApaLI        4777  GTGCAC               G^TGCAC
AspI         2389  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        4146  TGCGCA               TGC^GCA
BbeI         3925  GGCGCC               GGCGC^C
BbrPI        4780  CACGTG               CAC^GTG
BglII        4209  AGATCT               A^GATCT
BsaAI        4780  CACGTG               [CT]AC^GT[GA]
BsaHI        3925  GGCGCC               G[GA]^CG[CT]C
BsaI          572  GAGACC               GGTCTC(1/5)
BsaWI        3294  TCCGGA               [AT]^CCGG[AT]
BseAI        3294  TCCGGA               T^CCGGA
BsmI         2102  GAATGC               GAATGC(1/-1)
BspEI        3294  TCCGGA               T^CCGGA
BsrBI        4858  CCGCTC               CCGCTC(-3/-3)
BsrFI        3256  ACCGGC               [GA]^CCGG[CT]
BssSI        4283  CTCGTG               CACGAG(-5/-1)
Bst1107I     1039  GTATAC               GTA^TAC
BstEII        956  GGTGACC              G^GT[ACGT]ACC
BstPI         956  GGTGACC              G^GT[ACGT]ACC
BstZ17I      1039  GTATAC               GTA^TAC
Cfr10I       3256  ACCGGC               [GA]^CCGG[CT]
EcoO65I       956  GGTGACC              G^GT[ACGT]ACC
EcoRI         418  GAATTC               G^AATTC
FspI         4146  TGCGCA               TGC^GCA
Hin1I        3925  GGCGCC               G[GA]^CG[CT]C
HindIII      1742  AAGCTT               A^AGCTT
HpaI         1825  GTTAAC               GTT^AAC
KasI         3925  GGCGCC               G^GCGCC
MroI         3294  TCCGGA               T^CCGGA
NarI         3925  GGCGCC               GG^CGCC
PaeR7I        617  CTCGAG               C^TCGAG
PflFI        2389  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         895  CCACAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        4780  CACGTG               CAC^GTG
PmlI         4780  CACGTG               CAC^GTG
PshAI        3389  GACCTCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI         4378  GCTCTTC              GCTCTTC(1/4)
ScaI          774  AGTACT               AGT^ACT
SexAI        3268  ACCAGGT              A^CC[AT]GGT
SfiI         2641  GGCCATCACGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         3925  GGCGCC               GGC^GCC
SmaI         2376  CCCGGG               CCC^GGG
Tth111I      2389  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        895  CCACAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          617  CTCGAG               C^TCGAG
XmaCI        2376  CCCGGG               C^CCGGG
XmaI         2376  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

Acc65I        227  GGTACC               G^GTACC
              830  GGTACC              
AflIII        693  ACATGT               A^C[GA][CT]GT
             2036  ACATGT              
Aor51HI      1194  AGCGCT               AGC^GCT
             2445  AGCGCT              
Asp718I       227  GGTACC               G^GTACC
              830  GGTACC              
AvaI          617  CTCGAG               C^[CT]CG[GA]G
             2376  CCCGGG              
BamHI         448  GGATCC               G^GATCC
             1498  GGATCC              
BglI         2642  GCCATCACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4686  GCCCCAGTGGC         
BsoBI         617  CTCGAG               C^[CT]CG[GA]G
             2376  CCCGGG              
BspLU11I      693  ACATGT               A^CATGT
             2036  ACATGT              
BstXI        2180  CCAAATTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4639  CCAGGCCTCTGG        
Eco47III     1194  AGCGCT               AGC^GCT
             2445  AGCGCT              
EcoNI        1228  CCTAACCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4525  CCTGAAGAAGG         
HincII       1379  GTCAAC               GT[CT]^[GA]AC
             1825  GTTAAC              
HindII       1379  GTCAAC               GT[CT]^[GA]AC
             1825  GTTAAC              
KpnI          227  GGTACC               GGTAC^C
              830  GGTACC              


#########################################
# 3 OR MORE CUT ENZYMES (  119 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwI        AlwNI       AocI        ApoI        
Asp700I     AspHI       AvaII       BalI        BanI        
BanII       BbsI        BbvCI       BbvI        BcgI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       Bsp1286I    
BspMI       BsrDI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco81I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XcmI        XhoII       XmnI