##################################
# NO CUT ENZYMES (   53 enzymes) #
##################################

AccI        AhdI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        AspEI       AspI        
AviII       AvrII       BclI        BlnI        BsiWI       
BspLU11I    BssHII      Bst1107I    BstZ17I     CpoI        
DrdI        Eam1105I    Eco47III    EcoRV       EcoT22I     
FbaI        FspI        HincII      HindII      HpaI        
MfeI        MluI        MunI        NdeI        NheI        
NotI        NruI        NsiI        PacI        PflFI       
PmeI        PshAI       PshBI       PvuI        RsrII       
SalI        SnaBI       SpeI        SphI        SspI        
SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AatII        1188  GACGTC               GACGT^C
Acc65I       1436  GGTACC               G^GTACC
AccII          28  CGCG                 CG^CG
AccIII        327  TCCGGA               T^CCGGA
AclI         3733  AACGTT               AA^CGTT
AflII        4564  CTTAAG               C^TTAAG
AflIII        500  ACACGT               A^C[GA][CT]GT
AgeI         3615  ACCGGT               A^CCGGT
Asp700I       476  GAACCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1436  GGTACC               G^GTACC
BamHI        4136  GGATCC               G^GATCC
BbrPI         501  CACGTG               CAC^GTG
BcgI         1310  GCAAGGACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         4564  CTTAAG               C^TTAAG
BsaAI         501  CACGTG               [CT]AC^GT[GA]
BsaBI         694  GATCAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         327  TCCGGA               T^CCGGA
BsiEI         929  CGGCCG               CG[GA][CT]^CG
Bsp1407I     4832  TGTACA               T^GTACA
BspDI         524  ATCGAT               AT^CGAT
BspEI         327  TCCGGA               T^CCGGA
BsrGI        4832  TGTACA               T^GTACA
BssSI        2592  CACGAG               CACGAG(-5/-1)
BstAPI       3125  GCAGACAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         264  TTCGAA               TT^CGAA
BstUI          28  CGCG                 CG^CG
ClaI          524  ATCGAT               AT^CGAT
DraI         4784  TTTAAA               TTT^AAA
DraIII       3839  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          929  CGGCCG               C^GGCCG
EclXI         929  CGGCCG               C^GGCCG
Eco52I        929  CGGCCG               C^GGCCG
EcoRI         267  GAATTC               G^AATTC
FseI          930  GGCCGGCC             GGCCGG^CC
HindIII      2172  AAGCTT               A^AGCTT
KpnI         1436  GGTACC               GGTAC^C
KspI           27  CCGCGG               CCGC^GG
MamI          694  GATCAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          327  TCCGGA               T^CCGGA
MvnI           28  CGCG                 CG^CG
NspI         2432  GCATGT               [GA]CATG^[CT]
NspV          264  TTCGAA               TT^CGAA
PaeR7I       1203  CTCGAG               C^TCGAG
PinAI        3615  ACCGGT               A^CCGGT
PmaCI         501  CACGTG               CAC^GTG
PmlI          501  CACGTG               CAC^GTG
Psp1406I     3733  AACGTT               AA^CGTT
SacII          27  CCGCGG               CCGC^GG
SapI         1281  GAAGAGC              GCTCTTC(1/4)
SexAI        3954  ACCTGGT              A^CC[AT]GGT
SfiI           82  GGCCATGACGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI          264  TTCGAA               TT^CGAA
SgrAI        1607  CGCCGGTG             C[GA]^CCGG[CT]G
SmaI         4387  CCCGGG               CCC^GGG
SspBI        4832  TGTACA               T^GTACA
XhoI         1203  CTCGAG               C^TCGAG
XmaCI        4387  CCCGGG               C^CCGGG
XmaI         4387  CCCGGG               C^CCGGG
XmnI          476  GAACCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AvaI         1203  CTCGAG               C^[CT]CG[GA]G
             4387  CCCGGG              
BalI         1235  TGGCCA               TGG^CCA
             2727  TGGCCA              
BbeI           44  GGCGCC               GGCGC^C
             2994  GGCGCC              
BbsI          144  GAAGAC               GAAGAC(2/6)
             4312  GAAGAC              
BciVI        1531  GGATAC               GTATCC(6/5)
             4254  GTATCC              
BglII         216  AGATCT               A^GATCT
              917  AGATCT              
BpuAI         144  GAAGAC               GAAGAC(2/6)
             4312  GAAGAC              
BsaWI         327  TCCGGA               [AT]^CCGG[AT]
             3615  ACCGGT              
BsmBI         196  GAGACG               CGTCTC(1/5)
             1186  GAGACG              
BsmI          244  GAATGC               GAATGC(1/-1)
             3350  GCATTC              
BsoBI        1203  CTCGAG               C^[CT]CG[GA]G
             4387  CCCGGG              
BspHI         385  TCATGA               T^CATGA
              513  TCATGA              
BsrBI        3892  CCGCTC               CCGCTC(-3/-3)
              940  GAGCGG              
BsrDI         778  CATTGC               GCAATG(2/0)
             4492  GCAATG              
BstXI        1733  CCAGTGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1802  CCACAGACTTGG        
HaeII          44  GGCGCC               [GA]GCGC^[CT]
             2994  GGCGCC              
HgaI         4556  GACGC                GACGC(5/10)
             2605  GCGTC               
KasI           44  GGCGCC               G^GCGCC
             2994  GGCGCC              
MluNI        1235  TGGCCA               TGG^CCA
             2727  TGGCCA              
MscI         1235  TGGCCA               TGG^CCA
             2727  TGGCCA              
MslI         3096  CACTGCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             3598  CACAGGGATG          
NarI           44  GGCGCC               GG^CGCC
             2994  GGCGCC              
NcoI           60  CCATGG               C^CATGG
             4111  CCATGG              
RcaI          385  TCATGA               T^CATGA
              513  TCATGA              
SbfI          874  CCTGCAGG             CCTGCA^GG
             4664  CCTGCAGG            
SfoI           44  GGCGCC               GGC^GCC
             2994  GGCGCC              
Sse8387I      874  CCTGCAGG             CCTGCA^GG
             4664  CCTGCAGG            


#########################################
# 3 OR MORE CUT ENZYMES (  119 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        ApaI        ApoI        
AspHI       AvaII       BanI        BanII       BbvCI       
BbvI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrFI       BsrI        BssKI       BstEII      BstF5I      
BstNI       BstPI       BstYI       Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspA1I      MspI        MvaI        
MwoI        NaeI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SmlI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII