##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AclI AgeI AhdI AocI
AscI AspEI AspI AviII BbrPI
BcgI BlpI Bpu1102I BsaAI BsiEI
BsiWI BsmBI BspDI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRV FseI
FspI HindIII HpaI MfeI MluI
MunI NaeI NgoMIV NheI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
SacI SalI SapI SbfI SfuI
SgrAI SnaBI SphI Sse8387I SwaI
Tth111I
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 1240 GGTACC G^GTACC
AccI 624 GTCTAC GT^[AC][GT]AC
AccII 225 CGCG CG^CG
AccIII 2296 TCCGGA T^CCGGA
AflII 3522 CTTAAG C^TTAAG
Alw44I 2492 GTGCAC G^TGCAC
Aor51HI 1246 AGCGCT AGC^GCT
ApaI 1314 GGGCCC GGGCC^C
ApaLI 2492 GTGCAC G^TGCAC
AseI 4136 ATTAAT AT^TAAT
AsnI 4136 ATTAAT AT^TAAT
Asp718I 1240 GGTACC G^GTACC
AvrII 2813 CCTAGG C^CTAGG
BamHI 490 GGATCC G^GATCC
BbvCI 695 CCTCAGC CCTCAGC(-5/-2)
BfrI 3522 CTTAAG C^TTAAG
BlnI 2813 CCTAGG C^CTAGG
BsaBI 3736 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2296 TCCGGA [AT]^CCGG[AT]
BseAI 2296 TCCGGA T^CCGGA
BspEI 2296 TCCGGA T^CCGGA
BspHI 3421 TCATGA T^CATGA
BstAPI 2962 GCAAATATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 225 CGCG CG^CG
CpoI 2293 CGGTCCG CG^G[AT]CCG
Eco47III 1246 AGCGCT AGC^GCT
EcoNI 2345 CCTAGTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2268 ATGCAT ATGCA^T
FauI 642 GCGGG CCCGC(4/6)
HgaI 4659 GCGTC GACGC(5/10)
KpnI 1240 GGTACC GGTAC^C
KspI 224 CCGCGG CCGC^GG
MaeII 1606 ACGT A^CGT
MamI 3736 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2296 TCCGGA T^CCGGA
MvnI 225 CGCG CG^CG
NdeI 4230 CATATG CA^TATG
NsiI 2268 ATGCAT ATGCA^T
PaeR7I 2194 CTCGAG C^TCGAG
PshBI 4136 ATTAAT AT^TAAT
PvuII 2364 CAGCTG CAG^CTG
RcaI 3421 TCATGA T^CATGA
RsrII 2293 CGGTCCG CG^G[AT]CCG
SacII 224 CCGCGG CCGC^GG
ScaI 4164 AGTACT AGT^ACT
SexAI 1236 ACCTGGT A^CC[AT]GGT
SmaI 1815 CCCGGG CCC^GGG
SpeI 3757 ACTAGT A^CTAGT
SspI 2965 AATATT AAT^ATT
StuI 662 AGGCCT AGG^CCT
XbaI 2756 TCTAGA T^CTAGA
XhoI 2194 CTCGAG C^TCGAG
XmaCI 1815 CCCGGG C^CCGGG
XmaI 1815 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 580 ACATGT A^C[GA][CT]GT
1559 ACATGT
AlwNI 1950 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2202 CAGGTCCTG
Asp700I 1160 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3529 GAACTGTTTC
BalI 470 TGGCCA TGG^CCA
4714 TGGCCA
BclI 1999 TGATCA T^GATCA
3031 TGATCA
BsaI 450 GAGACC GGTCTC(1/5)
3417 GGTCTC
Bsp1407I 4065 TGTACA T^GTACA
4702 TGTACA
BspLU11I 580 ACATGT A^CATGT
1559 ACATGT
BsrGI 4065 TGTACA T^GTACA
4702 TGTACA
DraIII 256 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3103 CACATTGTG
EcoRI 837 GAATTC G^AATTC
1206 GAATTC
FbaI 1999 TGATCA T^GATCA
3031 TGATCA
HincII 2330 GTTGAC GT[CT]^[GA]AC
4024 GTCAAC
HindII 2330 GTTGAC GT[CT]^[GA]AC
4024 GTCAAC
MluNI 470 TGGCCA TGG^CCA
4714 TGGCCA
MscI 470 TGGCCA TGG^CCA
4714 TGGCCA
PshAI 579 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3622 GACTGAAGTC
PstI 1891 CTGCAG CTGCA^G
4583 CTGCAG
SfiI 344 GGCCAAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
515 GGCCCTGATGGCC
SspBI 4065 TGTACA T^GTACA
4702 TGTACA
XcmI 939 CCACACTATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3801 CCACATTTTCCCTGG
XmnI 1160 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3529 GAACTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BanI BanII BbeI BbsI BbvI
BciVI BcnI BfaI BglI BglII
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII