##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

AccI        AccII       AccIII      AclI        AgeI        
AhdI        Alw44I      ApaI        ApaLI       AscI        
AspEI       AvrII       BalI        BbeI        BbrPI       
BcgI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaI        BseAI       BsiEI       BsiWI       
BsmI        BspDI       BspEI       BsrFI       BssHII      
BssSI       Bst1107I    BstBI       BstUI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco52I      
EcoRI       EcoRV       FseI        KasI        KspI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        MvnI        NaeI        NarI        NcoI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       Sse8387I    Van91I      
XhoI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AatII        3548  GACGTC               GACGT^C
Aor51HI      3598  AGCGCT               AGC^GCT
AseI         4045  ATTAAT               AT^TAAT
AsnI         4045  ATTAAT               AT^TAAT
AspHI         369  GAGCTC               G[AT]GC[AT]^C
AspI           77  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         2823  CCCGGG               C^[CT]CG[GA]G
AviII        3962  TGCGCA               TGC^GCA
BbvCI          19  CCTCAGC              CCTCAGC(-5/-2)
BsaWI        2848  TCCGGT               [AT]^CCGG[AT]
BsiHKAI       369  GAGCTC               G[AT]GC[AT]^C
BsoBI        2823  CCCGGG               C^[CT]CG[GA]G
BspMI         592  GCAGGT               ACCTGC(4/8)
BsrBI        1651  GAGCGG               CCGCTC(-3/-3)
BstEII        574  GGTTACC              G^GT[ACGT]ACC
BstPI         574  GGTTACC              G^GT[ACGT]ACC
DraIII       1888  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          846  CCACGG               C^C[GA][CT]GG
Eco47III     3598  AGCGCT               AGC^GCT
EcoNI        4249  CCTGAGACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       574  GGTTACC              G^GT[ACGT]ACC
FauI         1938  GCGGG                CCCGC(4/6)
FspI         3962  TGCGCA               TGC^GCA
HaeII        3598  AGCGCT               [GA]GCGC^[CT]
HgaI         1656  GCGTC                GACGC(5/10)
NdeI         3387  CATATG               CA^TATG
PflFI          77  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        3565  GGGTCCT              [GA]G^G[AT]CC[CT]
PshAI        1359  GACCTTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        4045  ATTAAT               AT^TAAT
PstI          519  CTGCAG               CTGCA^G
SacI          369  GAGCTC               GAGCT^C
SapI          541  GCTCTTC              GCTCTTC(1/4)
SmaI         2823  CCCGGG               CCC^GGG
StuI         2808  AGGCCT               AGG^CCT
SwaI         3456  ATTTAAAT             ATTT^AAAT
Tth111I        77  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         2641  CCAAGTGTTTCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        2823  CCCGGG               C^CCGGG
XmaI         2823  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AcyI         1655  GGCGTC               G[GA]^CG[CT]C
             3548  GACGTC              
AocI          798  CCTCAGG              CC^T[ACGT]AGG
             2401  CCTAAGG             
Asp700I       189  GAAACCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              377  GAAGTACTTC          
BamHI        3675  GGATCC               G^GATCC
             4351  GGATCC              
BciVI         967  GGATAC               GTATCC(6/5)
             2930  GGATAC              
BsaHI        1655  GGCGTC               G[GA]^CG[CT]C
             3548  GACGTC              
BsmBI        3550  CGTCTC               CGTCTC(1/5)
             3546  GAGACG              
Bsp1407I     2191  TGTACA               T^GTACA
             3762  TGTACA              
BsrGI        2191  TGTACA               T^GTACA
             3762  TGTACA              
BstAPI       1571  GCATGCATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2136  GCATGTCTTGC         
BstXI        3555  CCATGTTAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3862  CCACTGATTTGG        
Bsu36I        798  CCTCAGG              CC^T[ACGT]AGG
             2401  CCTAAGG             
CfoI         3599  GCGC                 GCG^C
             3963  GCGC                
DraII        2826  GGGGCCT              [GA]G^G[ACGT]CC[CT]
             3565  GGGTCCT             
EarI          542  CTCTTC               CTCTTC(1/4)
             4929  GAAGAG              
Eco81I        798  CCTCAGG              CC^T[ACGT]AGG
             2401  CCTAAGG             
EcoO109I     2826  GGGGCCT              [GA]G^G[ACGT]CC[CT]
             3565  GGGTCCT             
HhaI         3599  GCGC                 GCG^C
             3963  GCGC                
Hin1I        1655  GGCGTC               G[GA]^CG[CT]C
             3548  GACGTC              
HinP1I       3599  GCGC                 G^CGC
             3963  GCGC                
HpaI         1146  GTTAAC               GTT^AAC
             3043  GTTAAC              
Ksp632I       542  CTCTTC               CTCTTC(1/4)
             4929  GAAGAG              
MspA1I       1509  CAGCTG               C[AC]G^C[GT]G
             4070  CAGCTG              
PvuII        1509  CAGCTG               CAG^CTG
             4070  CAGCTG              
SphI         1571  GCATGC               GCATG^C
             3630  GCATGC              
SspBI        2191  TGTACA               T^GTACA
             3762  TGTACA              
TaqI            9  TCGA                 T^CGA
             1931  TCGA                
TthHB8I         9  TCGA                 T^CGA
             1931  TCGA                
XbaI         1706  TCTAGA               T^CTAGA
             3007  TCTAGA              
XmnI          189  GAAACCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              377  GAAGTACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

Acc65I      AciI        AcsI        AfaI        AflII       
AflIII      AluI        AlwI        AlwNI       ApoI        
Asp718I     AvaII       BanI        BanII       BbsI        
BbvI        BclI        BcnI        BfaI        BfrI        
BmyI        BpmI        BpuAI       BsaAI       BsaBI       
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspHI       BspLU11I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraI        EcoRII      EcoT14I     EcoT22I     FbaI        
Fnu4HI      FokI        HaeIII      HapII       HincII      
HindII      HindIII     HinfI       HpaII       HphI        
ItaI        KpnI        MaeI        MaeII       MaeIII      
MamI        MboI        MboII       MflI        MnlI        
MseI        MslI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PleI        RcaI        RsaI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SmlI        SnaBI       SpeI        SspI        StyI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XhoII