##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AhdI        
Alw44I      Aor51HI     ApaLI       AscI        Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BlnI        BsaAI       
BsaBI       BsaHI       BseAI       BsiEI       BsiWI       
BsmBI       BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BssHII      BssSI       Bst1107I    BstEII      
BstPI       BstZ17I     CfoI        ClaI        CpoI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     EcoT22I     FauI        
FseI        FspI        HaeII       HhaI        Hin1I       
HinP1I      HpaI        KasI        KpnI        KspI        
MamI        MluI        MroI        NaeI        NarI        
NgoMIV      NotI        NruI        NsiI        PacI        
PflFI       PmaCI       PmeI        PmlI        PshAI       
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        ScaI        SfoI        SgrAI       SmaI        
SnaBI       SphI        Sse8387I    Tth111I     XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccII         232  CGCG                 CG^CG
AclI         4430  AACGTT               AA^CGTT
AflII        2958  CTTAAG               C^TTAAG
AgeI         4154  ACCGGT               A^CCGGT
AocI         5343  CCTTAGG              CC^T[ACGT]AGG
ApaI         2677  GGGCCC               GGGCC^C
AvaI          974  CTCGAG               C^[CT]CG[GA]G
BcgI         3972  GCAGGTGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI         4508  CCCGG                CC^[GC]GG
BfrI         2958  CTTAAG               C^TTAAG
BglI         4730  GCCATAAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         3147  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     3147  GCTCAGC              GC^T[ACGT]AGC
BsmFI        3609  GGGAC                GGGAC(10/14)
BsoBI         974  CTCGAG               C^[CT]CG[GA]G
Bsp1407I     5707  TGTACA               T^GTACA
BsrDI        2024  GCAATG               GCAATG(2/0)
BsrFI        4154  ACCGGT               [GA]^CCGG[CT]
BsrGI        5707  TGTACA               T^GTACA
BstUI         232  CGCG                 CG^CG
BstXI        4282  CCAACAAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       5343  CCTTAGG              CC^T[ACGT]AGG
CelII        3147  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       4154  ACCGGT               [GA]^CCGG[CT]
Eco81I       5343  CCTTAGG              CC^T[ACGT]AGG
EcoNI        3883  CCTACAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI         3939  GCGTC                GACGC(5/10)
HindIII      3294  AAGCTT               A^AGCTT
MfeI         3098  CAATTG               C^AATTG
MunI         3098  CAATTG               C^AATTG
MvnI          232  CGCG                 CG^CG
NciI         4508  CCCGG                CC^[GC]GG
NspI         1274  GCATGT               [GA]CATG^[CT]
PaeR7I        974  CTCGAG               C^TCGAG
PinAI        4154  ACCGGT               A^CCGGT
Psp1406I     4430  AACGTT               AA^CGTT
SapI         3928  GAAGAGC              GCTCTTC(1/4)
SfiI         4729  GGCCATAAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI         3616  ACTAGT               A^CTAGT
SspBI        5707  TGTACA               T^GTACA
StuI         4737  AGGCCT               AGG^CCT
SwaI         2666  ATTTAAAT             ATTT^AAAT
XhoI          974  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccI         2595  GTCTAC               GT^[AC][GT]AC
             6794  GTCTAC              
AflIII       2715  ACGTGT               A^C[GA][CT]GT
             5125  ACGTGT              
BalI         4405  TGGCCA               TGG^CCA
             5921  TGGCCA              
BanI         2611  GGCACC               G^G[CT][GA]CC
             4325  GGCACC              
BciVI         968  GGATAC               GTATCC(6/5)
              195  GTATCC              
BglII        1963  AGATCT               A^GATCT
             2696  AGATCT              
BstAPI        877  GCAGCTACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2355  GCAAAGTCTGC         
BstBI        1347  TTCGAA               TT^CGAA
             4649  TTCGAA              
EcoRI         759  GAATTC               G^AATTC
             6649  GAATTC              
EcoRV        1556  GATATC               GAT^ATC
             3819  GATATC              
MluNI        4405  TGGCCA               TGG^CCA
             5921  TGGCCA              
MscI         4405  TGGCCA               TGG^CCA
             5921  TGGCCA              
NdeI         2113  CATATG               CA^TATG
             5314  CATATG              
NheI         2011  GCTAGC               G^CTAGC
             2337  GCTAGC              
NspV         1347  TTCGAA               TT^CGAA
             4649  TTCGAA              
PvuII        3180  CAGCTG               CAG^CTG
             4412  CAGCTG              
SacI         3391  GAGCTC               GAGCT^C
             5653  GAGCTC              
SexAI        4016  ACCTGGT              A^CC[AT]GGT
             5974  ACCTGGT             
SfuI         1347  TTCGAA               TT^CGAA
             4649  TTCGAA              
XbaI         2256  TCTAGA               T^CTAGA
             6624  TCTAGA              
XcmI         1897  CCAGATCCTGACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             5935  CCAGGGGCTAGTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  103 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AseI        AsnI        Asp700I     
AspHI       AvaII       BanII       BbsI        BbvI        
BclI        BfaI        BmyI        BpmI        BpuAI       
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmI        
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FbaI        
Fnu4HI      FokI        HaeIII      HapII       HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NcoI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PpuMI       PshBI       PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XhoII       XmnI