##################################
# NO CUT ENZYMES (   50 enzymes) #
##################################

AatII       AccI        AflII       AgeI        Alw44I      
Aor51HI     ApaLI       AscI        AvrII       BbeI        
BfrI        BglI        BlnI        BsaBI       BspDI       
BssHII      Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        Eco47III    EcoT22I     HindIII     HpaI        
KasI        KspI        MamI        MluI        NarI        
NheI        NotI        NsiI        NspV        PacI        
PinAI       PmeI        PshAI       PvuI        RsrII       
SacII       SalI        SbfI        SfiI        SfoI        
SfuI        SpeI        Sse8387I    SwaI        XcmI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

Acc65I       4430  GGTACC               G^GTACC
AccIII       1338  TCCGGA               T^CCGGA
AclI         1751  AACGTT               AA^CGTT
AcyI         2733  GACGCC               G[GA]^CG[CT]C
AhdI         1981  GACCAGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        1035  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI           22  GGGCCC               GGGCC^C
AseI         3314  ATTAAT               AT^TAAT
AsnI         3314  ATTAAT               AT^TAAT
Asp718I      4430  GGTACC               G^GTACC
AspEI        1981  GACCAGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII          16  TGCGCA               TGC^GCA
BalI         2050  TGGCCA               TGG^CCA
BamHI        2347  GGATCC               G^GATCC
BbrPI        4456  CACGTG               CAC^GTG
BciVI        2746  GTATCC               GTATCC(6/5)
BglII        3952  AGATCT               A^GATCT
BlpI         4197  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     4197  GCTGAGC              GC^T[ACGT]AGC
BsaHI        2733  GACGCC               G[GA]^CG[CT]C
BseAI        1338  TCCGGA               T^CCGGA
BsiEI        1592  CGGCCG               CG[GA][CT]^CG
BsiWI        1457  CGTACG               C^GTACG
BsmBI         424  GAGACG               CGTCTC(1/5)
BsmI         2657  GCATTC               GAATGC(1/-1)
BspEI        1338  TCCGGA               T^CCGGA
BspHI        4088  TCATGA               T^CATGA
BspLU11I     3779  ACATGT               A^CATGT
BssSI         421  CACGAG               CACGAG(-5/-1)
BstEII        104  GGTTACC              G^GT[ACGT]ACC
BstPI         104  GGTTACC              G^GT[ACGT]ACC
CelII        4197  GCTGAGC              GC^T[ACGT]AGC
EagI         1592  CGGCCG               C^GGCCG
Eam1105I     1981  GACCAGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1592  CGGCCG               C^GGCCG
Eco52I       1592  CGGCCG               C^GGCCG
EcoO65I       104  GGTTACC              G^GT[ACGT]ACC
EcoRI        1732  GAATTC               G^AATTC
EcoRV        1899  GATATC               GAT^ATC
FseI         1593  GGCCGGCC             GGCCGG^CC
FspI           16  TGCGCA               TGC^GCA
Hin1I        2733  GACGCC               G[GA]^CG[CT]C
KpnI         4430  GGTACC               GGTAC^C
MfeI         4153  CAATTG               C^AATTG
MluNI        2050  TGGCCA               TGG^CCA
MroI         1338  TCCGGA               T^CCGGA
MscI         2050  TGGCCA               TGG^CCA
MunI         4153  CAATTG               C^AATTG
NruI         2778  TCGCGA               TCG^CGA
PaeR7I        541  CTCGAG               C^TCGAG
PmaCI        4456  CACGTG               CAC^GTG
PmlI         4456  CACGTG               CAC^GTG
PpuMI        4662  AGGACCT              [GA]G^G[AT]CC[CT]
PshBI        3314  ATTAAT               AT^TAAT
Psp1406I     1751  AACGTT               AA^CGTT
RcaI         4088  TCATGA               T^CATGA
SapI          224  GCTCTTC              GCTCTTC(1/4)
SgrAI         858  CACCGGCG             C[GA]^CCGG[CT]G
SnaBI         270  TACGTA               TAC^GTA
SphI         4307  GCATGC               GCATG^C
XbaI          185  TCTAGA               T^CTAGA
XhoI          541  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccII          62  CGCG                 CG^CG
             2779  CGCG                
AspI         2487  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4559  GACCCTGTC           
BbsI          891  GAAGAC               GAAGAC(2/6)
             1791  GAAGAC              
BbvCI        1400  CCTCAGC              CCTCAGC(-5/-2)
             2826  CCTCAGC             
BcgI         1154  CGACGCAGTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             4474  GCAGAATTGTCG        
BpuAI         891  GAAGAC               GAAGAC(2/6)
             1791  GAAGAC              
BsaAI         270  TACGTA               [CT]AC^GT[GA]
             4456  CACGTG              
BsaI          127  GAGACC               GGTCTC(1/5)
              942  GAGACC              
BsrDI        4470  CATTGC               GCAATG(2/0)
             2846  GCAATG              
BstAPI       2991  GCACCCTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3024  GCAAGTTCTGC         
BstUI          62  CGCG                 CG^CG
             2779  CGCG                
BstXI          94  CCAGGCTGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1560  CCACCCGGCTGG        
DraI         1685  TTTAAA               TTT^AAA
             3357  TTTAAA              
DrdI          523  GACCAAGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4525  GACCCTGCTGTC        
MslI         1737  CACTTGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2948  CATGGCCGTG          
MvnI           62  CGCG                 CG^CG
             2779  CGCG                
NaeI         1387  GCCGGC               GCC^GGC
             1594  GCCGGC              
NcoI          484  CCATGG               C^CATGG
             4721  CCATGG              
NdeI         1665  CATATG               CA^TATG
             2911  CATATG              
NgoMIV       1387  GCCGGC               G^CCGGC
             1594  GCCGGC              
PflFI        2487  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4559  GACCCTGTC           
PvuII        2829  CAGCTG               CAG^CTG
             3086  CAGCTG              
SexAI        3151  ACCTGGT              A^CC[AT]GGT
             4842  ACCTGGT             
SmaI            2  CCCGGG               CCC^GGG
              711  CCCGGG              
StuI         2159  AGGCCT               AGG^CCT
             4766  AGGCCT              
Tth111I      2487  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4559  GACCCTGTC           
XmaCI           2  CCCGGG               C^CCGGG
              711  CCCGGG              
XmaI            2  CCCGGG               C^CCGGG
              711  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AocI        ApoI        Asp700I     AspHI       
AvaI        AvaII       BanI        BanII       BbvI        
BclI        BcnI        BfaI        BmyI        BpmI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    Bsp1407I    BspMI       BsrBI       BsrFI       
BsrGI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EarI        Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        FbaI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PstI        
RsaI        SacI        Sau3AI      Sau96I      ScaI        
ScrFI       SfaNI       SfcI        SmlI        SspBI       
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XhoII       XmnI