##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp718I AspEI AviII BamHI
BbrPI BciVI BclI BfrI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FbaI FseI FspI HpaI
KpnI KspI MfeI MluI MroI
MunI NaeI NgoMIV NheI NotI
NruI NspV PacI PflMI PinAI
PmaCI PmlI Psp1406I PvuI RcaI
RsrII SacII SapI SbfI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SphI Sse8387I StuI SwaI Van91I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccII 936 CGCG CG^CG
ApaI 3955 GGGCCC GGGCC^C
AspHI 100 GAGCTC G[AT]GC[AT]^C
BalI 2932 TGGCCA TGG^CCA
BbeI 4247 GGCGCC GGCGC^C
BglI 4239 GCCGAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1276 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1276 GCTTAGC GC^T[ACGT]AGC
BsaAI 4161 TACGTG [CT]AC^GT[GA]
BsaBI 4381 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 949 ACCGGA [AT]^CCGG[AT]
BsiHKAI 100 GAGCTC G[AT]GC[AT]^C
BsmBI 4127 CGTCTC CGTCTC(1/5)
BspMI 30 ACCTGC ACCTGC(4/8)
BstUI 936 CGCG CG^CG
CelII 1276 GCTTAGC GC^T[ACGT]AGC
DraIII 3326 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3228 CCATGG C^C[GA][CT]GG
EcoNI 2064 CCTTCTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 4070 GCGGG CCCGC(4/6)
HgaI 1103 GACGC GACGC(5/10)
KasI 4247 GGCGCC G^GCGCC
MamI 4381 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2932 TGGCCA TGG^CCA
MscI 2932 TGGCCA TGG^CCA
MvnI 936 CGCG CG^CG
NarI 4247 GGCGCC GG^CGCC
NcoI 3228 CCATGG C^CATGG
PmeI 271 GTTTAAAC GTTT^AAAC
PpuMI 4637 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 84 GACTGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 100 GAGCTC GAGCT^C
SalI 1880 GTCGAC G^TCGAC
ScaI 765 AGTACT AGT^ACT
SfoI 4247 GGCGCC GGC^GCC
SpeI 4827 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 1880 GTCGAC GT^[AC][GT]AC
4745 GTAGAC
AcyI 1103 GACGCC G[GA]^CG[CT]C
4247 GGCGCC
AflIII 2448 ACATGT A^C[GA][CT]GT
3193 ACATGT
AspI 1175 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4282 GACTCTGTC
BanI 4247 GGCGCC G^G[CT][GA]CC
4480 GGTGCC
BbvCI 3103 GCTGAGG CCTCAGC(-5/-2)
4191 GCTGAGG
BcgI 1035 GCAGATGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4197 GCAGGAGAATCG
BcnI 4021 CCGGG CC^[GC]GG
4154 CCGGG
BsaHI 1103 GACGCC G[GA]^CG[CT]C
4247 GGCGCC
BspLU11I 2448 ACATGT A^CATGT
3193 ACATGT
BstXI 1453 CCAAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4049 CCAGCACTTTGG
Cac8I 214 GCTTGC GC[ACGT]^[ACGT]GC
1166 GCTTGC
EaeI 530 CGGCCA [CT]^GGCC[GA]
2932 TGGCCA
Hin1I 1103 GACGCC G[GA]^CG[CT]C
4247 GGCGCC
HincII 1880 GTCGAC GT[CT]^[GA]AC
4109 GTCAAC
HindII 1880 GTCGAC GT[CT]^[GA]AC
4109 GTCAAC
HindIII 1594 AAGCTT A^AGCTT
1733 AAGCTT
MaeII 1884 ACGT A^CGT
4162 ACGT
NciI 4021 CCGGG CC^[GC]GG
4154 CCGGG
PaeR7I 654 CTCGAG C^TCGAG
3826 CTCGAG
PflFI 1175 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4282 GACTCTGTC
Tth111I 1175 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4282 GACTCTGTC
XbaI 1638 TCTAGA T^CTAGA
1839 TCTAGA
XhoI 654 CTCGAG C^TCGAG
3826 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AseI AsnI
Asp700I AvaI AvaII AvrII BanII
BbsI BbvI BfaI BglII BlnI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstYI Bsu36I CfoI
Cfr13I DdeI DpnII DraI DraII
EarI Eco81I EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeI NdeII NlaIII NlaIV NsiI
NspI PleI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI