##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AhdI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BciVI       BfrI        BglII       BlnI        BseAI       
BsiWI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrGI       BssHII      Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        Eam1105I    EcoRI       
EcoRV       FseI        FspI        HpaI        KpnI        
MfeI        MluI        MroI        MunI        NdeI        
NotI        NruI        NspV        PacI        PflFI       
PmeI        PshBI       Psp1406I    PvuI        RcaI        
RsrII       SapI        ScaI        SexAI       SfuI        
SgrAI       SnaBI       SpeI        SspBI       Tth111I     
XbaI        XcmI        


###################################
# ONE CUT ENZYMES (   23 enzymes) #
###################################

AgeI         2310  ACCGGT               A^CCGGT
Aor51HI       520  AGCGCT               AGC^GCT
ApaI         3096  GGGCCC               GGGCC^C
BcgI         4676  GCAATGATCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         4342  TGATCA               T^GATCA
BstEII        745  GGTCACC              G^GT[ACGT]ACC
BstPI         745  GGTCACC              G^GT[ACGT]ACC
DraIII       2606  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         4496  GACTCTGCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      520  AGCGCT               AGC^GCT
EcoO65I       745  GGTCACC              G^GT[ACGT]ACC
FbaI         4342  TGATCA               T^GATCA
HindIII      3611  AAGCTT               A^AGCTT
KspI         2466  CCGCGG               CCGC^GG
NcoI         3259  CCATGG               C^CATGG
PinAI        2310  ACCGGT               A^CCGGT
PshAI        2836  GACGGTCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII        2466  CCGCGG               CCGC^GG
SalI         2621  GTCGAC               G^TCGAC
SbfI          826  CCTGCAGG             CCTGCA^GG
SfiI         3097  GGCCCCCGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I      826  CCTGCAGG             CCTGCA^GG
SwaI         5212  ATTTAAAT             ATTT^AAAT


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AcsI           97  AAATTT               [GA]^AATT[CT]
             3505  AAATTT              
AflIII       2118  ACGTGT               A^C[GA][CT]GT
             2511  ACACGT              
Alw44I       2252  GTGCAC               G^TGCAC
             2348  GTGCAC              
ApaLI        2252  GTGCAC               G^TGCAC
             2348  GTGCAC              
ApoI           97  AAATTT               [GA]^AATT[CT]
             3505  AAATTT              
BbvCI        4098  CCTCAGC              CCTCAGC(-5/-2)
             4731  CCTCAGC             
BglI         3098  GCCCCCGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4822  GCCAGGTTGGC         
BsmBI        4171  GAGACG               CGTCTC(1/5)
             4632  GAGACG              
BstAPI       2131  GCACATCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4695  GCAACCTCTGC         
BstYI        1608  AGATCC               [GA]^GATC[CT]
             2642  GGATCT              
EagI         1756  CGGCCG               C^GGCCG
             2918  CGGCCG              
EclXI        1756  CGGCCG               C^GGCCG
             2918  CGGCCG              
Eco52I       1756  CGGCCG               C^GGCCG
             2918  CGGCCG              
MflI         1608  AGATCC               [GA]^GATC[CT]
             2642  GGATCT              
NheI         2756  GCTAGC               G^CTAGC
             3488  GCTAGC              
PaeR7I       1967  CTCGAG               C^TCGAG
             2855  CTCGAG              
SphI          571  GCATGC               GCATG^C
             1686  GCATGC              
XhoI         1967  CTCGAG               C^TCGAG
             2855  CTCGAG              
XhoII        1608  AGATCC               [GA]^GATC[CT]
             2642  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (  149 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       AocI        Asp700I     
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbeI        BbrPI       BbsI        BbvI        
BcnI        BfaI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspMI       
BsrBI       BsrDI       BsrFI       BsrI        BssKI       
BssSI       BstF5I      BstNI       BstUI       BstXI       
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EaeI        EarI        Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     EcoT22I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
KasI        Ksp632I     MaeI        MaeII       MaeIII      
MamI        MboI        MboII       MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
NsiI        NspI        PflMI       PleI        PmaCI       
PmlI        PpuMI       PstI        PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmaI        SmlI        SspI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XmaCI       XmaI        XmnI