##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BbeI        
BbrPI       BbvCI       BfrI        BglI        BlnI        
BsaHI       BsaI        BsiEI       BsiWI       Bsp1407I    
BspDI       BspHI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       BstBI       BstEII      BstPI       
BstUI       BstXI       Cfr10I      ClaI        CpoI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     EcoRV       FauI        
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HpaI        KasI        KpnI        KspI        
MluI        MluNI       MscI        MvnI        NaeI        
NarI        NcoI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SphI        Sse8387I    SspBI       SwaI        
Tth111I     Van91I      XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccI         1324  GTATAC               GT^[AC][GT]AC
AccIII        790  TCCGGA               T^CCGGA
AlwNI         102  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       365  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         4228  CTCGGG               C^[CT]CG[GA]G
BamHI        3574  GGATCC               G^GATCC
BcgI          681  CGAATATTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1793  GGATAC               GTATCC(6/5)
BglII        2916  AGATCT               A^GATCT
BlpI         3389  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     3389  GCTCAGC              GC^T[ACGT]AGC
BsaBI         407  GATTGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         790  TCCGGA               T^CCGGA
BsmBI        4141  CGTCTC               CGTCTC(1/5)
BsoBI        4228  CTCGGG               C^[CT]CG[GA]G
BspEI         790  TCCGGA               T^CCGGA
BspLU11I     2577  ACATGT               A^CATGT
BspMI        1553  ACCTGC               ACCTGC(4/8)
Bst1107I     1324  GTATAC               GTA^TAC
BstAPI       2975  GCAGCACATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I      1324  GTATAC               GTA^TAC
CelII        3389  GCTCAGC              GC^T[ACGT]AGC
CfoI          104  GCGC                 GCG^C
DrdI         3242  GACTGTGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        2366  CCTGTGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1211  GAATTC               G^AATTC
HaeII         103  AGCGCC               [GA]GCGC^[CT]
HhaI          104  GCGC                 GCG^C
HinP1I        104  GCGC                 G^CGC
MamI          407  GATTGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          790  TCCGGA               T^CCGGA
PshAI         302  GACTGGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         2342  CTGCAG               CTGCA^G
PvuII        4247  CAGCTG               CAG^CTG
SpeI         3290  ACTAGT               A^CTAGT
XmnI          365  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

BanI           11  GGTGCC               G^G[CT][GA]CC
             3410  GGTGCC              
BclI          412  TGATCA               T^GATCA
             1732  TGATCA              
BcnI           49  CCCGG                CC^[GC]GG
             4210  CCCGG               
BpmI         1077  CTCCAG               CTGGAG(16/14)
               22  CTGGAG              
BsaAI        1403  TACGTG               [CT]AC^GT[GA]
             3114  TACGTG              
BsaWI         736  ACCGGA               [AT]^CCGG[AT]
              790  TCCGGA              
BsgI         1061  CTGCAC               GTGCAG(16/14)
             1202  CTGCAC              
BsmI          781  GCATTC               GAATGC(1/-1)
             3186  GCATTC              
BsrDI        1641  CATTGC               GCAATG(2/0)
             2852  CATTGC              
DraIII       1308  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1825  CACACTGTG           
EcoT14I      2503  CCAAGG               C^C[AT][AT]GG
             3363  CCTTGG              
FbaI          412  TGATCA               T^GATCA
             1732  TGATCA              
MfeI          838  CAATTG               C^AATTG
             3818  CAATTG              
MslI         1118  CATATGTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2035  CATATGGATG          
MunI          838  CAATTG               C^AATTG
             3818  CAATTG              
NciI           49  CCCGG                CC^[GC]GG
             4210  CCCGG               
SacI         1288  GAGCTC               GAGCT^C
             4474  GAGCTC              
StyI         2503  CCAAGG               C^C[AT][AT]GG
             3363  CCTTGG              
TaqI         1342  TCGA                 T^CGA
             4342  TCGA                
TthHB8I      1342  TCGA                 T^CGA
             4342  TCGA                
XbaI         2317  TCTAGA               T^CTAGA
             2821  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AocI        ApoI        AseI        AsnI        
AspHI       AvaII       BanII       BbsI        BbvI        
BfaI        BmyI        BpuAI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Bsu36I      Cac8I       Cfr13I      DdeI        
DpnII       DraI        DraII       EarI        Eco81I      
EcoO109I    EcoRII      EcoT22I     Fnu4HI      FokI        
HaeIII      HapII       HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MspA1I      MspI        MvaI        MwoI        
NdeI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PleI        PpuMI       PshBI       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        StuI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XhoII