##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I AspEI AviII AvrII BfrI
BlnI BsaBI BseAI BsiWI BsmBI
BspDI BspEI BspLU11I BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FseI
FspI HindIII HpaI KspI MamI
MfeI MluI MroI MunI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI ScaI SfiI SfuI SnaBI
SpeI SphI SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 72 GACGTC GACGT^C
Acc65I 1272 GGTACC G^GTACC
AcsI 3294 GAATTT [GA]^AATT[CT]
AflIII 2061 ACGTGT A^C[GA][CT]GT
Alw44I 1278 GTGCAC G^TGCAC
ApaLI 1278 GTGCAC G^TGCAC
ApoI 3294 GAATTT [GA]^AATT[CT]
Asp718I 1272 GGTACC G^GTACC
AspI 1476 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 589 GGCGCC GGCGC^C
BcgI 2437 GCAACTGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 738 CTAG C^TAG
Bsp1407I 1543 TGTACA T^GTACA
BspHI 2881 TCATGA T^CATGA
BsrDI 2371 GCAATG GCAATG(2/0)
BsrGI 1543 TGTACA T^GTACA
BssHII 2897 GCGCGC G^CGCGC
BstEII 387 GGTCACC G^GT[ACGT]ACC
BstPI 387 GGTCACC G^GT[ACGT]ACC
EarI 3429 CTCTTC CTCTTC(1/4)
EcoO65I 387 GGTCACC G^GT[ACGT]ACC
HaeII 589 GGCGCC [GA]GCGC^[CT]
KasI 589 GGCGCC G^GCGCC
KpnI 1272 GGTACC GGTAC^C
Ksp632I 3429 CTCTTC CTCTTC(1/4)
MaeI 738 CTAG C^TAG
MseI 1852 TTAA T^TAA
NarI 589 GGCGCC GG^CGCC
NdeI 2909 CATATG CA^TATG
PflFI 1476 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2881 TCATGA T^CATGA
SacI 3113 GAGCTC GAGCT^C
SbfI 1619 CCTGCAGG CCTGCA^GG
SfoI 589 GGCGCC GGC^GCC
SgrAI 1491 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 1619 CCTGCAGG CCTGCA^GG
SspBI 1543 TGTACA T^GTACA
StuI 3013 AGGCCT AGG^CCT
Tru9I 1852 TTAA T^TAA
Tth111I 1476 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AocI 1794 CCTGAGG CC^T[ACGT]AGG
2891 CCTGAGG
AvaI 1360 CCCGGG C^[CT]CG[GA]G
3337 CCCGGG
BamHI 664 GGATCC G^GATCC
1789 GGATCC
BbrPI 1974 CACGTG CAC^GTG
2481 CACGTG
BbsI 759 GAAGAC GAAGAC(2/6)
1124 GAAGAC
BbvCI 14 GCTGAGG CCTCAGC(-5/-2)
2549 GCTGAGG
BciVI 2993 GGATAC GTATCC(6/5)
2589 GTATCC
BclI 460 TGATCA T^GATCA
2782 TGATCA
BglI 802 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1492 GCCGGCGTGGC
BglII 949 AGATCT A^GATCT
2758 AGATCT
BlpI 261 GCTCAGC GC^T[ACGT]AGC
1982 GCTCAGC
Bpu1102I 261 GCTCAGC GC^T[ACGT]AGC
1982 GCTCAGC
BpuAI 759 GAAGAC GAAGAC(2/6)
1124 GAAGAC
BsaAI 1974 CACGTG [CT]AC^GT[GA]
2481 CACGTG
BsiEI 1348 CGACCG CG[GA][CT]^CG
1652 CGACCG
BsoBI 1360 CCCGGG C^[CT]CG[GA]G
3337 CCCGGG
BstAPI 1768 GCATGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2495 GCAGATCCTGC
Bsu36I 1794 CCTGAGG CC^T[ACGT]AGG
2891 CCTGAGG
CelII 261 GCTCAGC GC^T[ACGT]AGC
1982 GCTCAGC
Eco81I 1794 CCTGAGG CC^T[ACGT]AGG
2891 CCTGAGG
FbaI 460 TGATCA T^GATCA
2782 TGATCA
HgaI 1197 GACGC GACGC(5/10)
1045 GCGTC
MslI 1386 CATGTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1970 CATCCACGTG
NaeI 1492 GCCGGC GCC^GGC
2491 GCCGGC
NcoI 2164 CCATGG C^CATGG
2306 CCATGG
NgoMIV 1492 GCCGGC G^CCGGC
2491 GCCGGC
PmaCI 1974 CACGTG CAC^GTG
2481 CACGTG
PmlI 1974 CACGTG CAC^GTG
2481 CACGTG
SmaI 1360 CCCGGG CCC^GGG
3337 CCCGGG
XmaCI 1360 CCCGGG C^CCGGG
3337 CCCGGG
XmaI 1360 CCCGGG C^CCGGG
3337 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaII
BalI BanI BanII BbvI BcnI
BmyI BpmI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII