##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccIII AclI AgeI Aor51HI
AscI AspI AviII BamHI BbrPI
BcgI BsaAI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BsrBI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Cfr10I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV FauI FseI FspI KspI
MfeI MroI MunI NaeI NcoI
NdeI NgoMIV NotI NruI NspV
PaeR7I PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SacII SalI SapI SexAI SfiI
SfuI SgrAI SnaBI SpeI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 677 GGTACC G^GTACC
AflII 1604 CTTAAG C^TTAAG
AhdI 3948 GACTAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 789 GGGCCC GGGCC^C
AseI 4027 ATTAAT AT^TAAT
AsnI 4027 ATTAAT AT^TAAT
Asp718I 677 GGTACC G^GTACC
AspEI 3948 GACTAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 5292 GTATCC GTATCC(6/5)
BfrI 1604 CTTAAG C^TTAAG
BglII 2158 AGATCT A^GATCT
BlpI 5117 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 5117 GCTGAGC GC^T[ACGT]AGC
BsaBI 530 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2310 GAATGC GAATGC(1/-1)
BspLU11I 1414 ACATGT A^CATGT
CelII 5117 GCTGAGC GC^T[ACGT]AGC
DraIII 554 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 217 CCACGG C^C[GA][CT]GG
Eam1105I 3948 GACTAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2179 CCTCCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4729 GAATTC G^AATTC
HpaI 2962 GTTAAC GTT^AAC
KpnI 677 GGTACC GGTAC^C
MamI 530 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 628 ACGCGT A^CGCGT
NheI 3745 GCTAGC G^CTAGC
PacI 2398 TTAATTAA TTAAT^TAA
PshAI 2835 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4027 ATTAAT AT^TAAT
SbfI 344 CCTGCAGG CCTGCA^GG
SmaI 415 CCCGGG CCC^GGG
SphI 2792 GCATGC GCATG^C
Sse8387I 344 CCTGCAGG CCTGCA^GG
StuI 2926 AGGCCT AGG^CCT
XcmI 214 CCACCACGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 415 CCCGGG C^CCGGG
XmaI 415 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 2130 GTAGAC GT^[AC][GT]AC
3555 GTAGAC
AccII 460 CGCG CG^CG
629 CGCG
AcyI 316 GGCGCC G[GA]^CG[CT]C
779 GGCGCC
Alw44I 1530 GTGCAC G^TGCAC
1835 GTGCAC
AocI 730 CCTGAGG CC^T[ACGT]AGG
1634 CCTTAGG
ApaLI 1530 GTGCAC G^TGCAC
1835 GTGCAC
Asp700I 1073 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3115 GAAATTTTTC
AvaI 243 CCCGAG C^[CT]CG[GA]G
415 CCCGGG
AvrII 1730 CCTAGG C^CTAGG
2608 CCTAGG
BbeI 316 GGCGCC GGCGC^C
779 GGCGCC
BclI 3880 TGATCA T^GATCA
4785 TGATCA
BglI 606 GCCGAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
782 GCCCCAAGGGC
BlnI 1730 CCTAGG C^CTAGG
2608 CCTAGG
BsaHI 316 GGCGCC G[GA]^CG[CT]C
779 GGCGCC
BsmBI 141 GAGACG CGTCTC(1/5)
5137 GAGACG
BsoBI 243 CCCGAG C^[CT]CG[GA]G
415 CCCGGG
BstUI 460 CGCG CG^CG
629 CGCG
Bsu36I 730 CCTGAGG CC^T[ACGT]AGG
1634 CCTTAGG
Eco81I 730 CCTGAGG CC^T[ACGT]AGG
1634 CCTTAGG
FbaI 3880 TGATCA T^GATCA
4785 TGATCA
HaeII 316 GGCGCC [GA]GCGC^[CT]
779 GGCGCC
Hin1I 316 GGCGCC G[GA]^CG[CT]C
779 GGCGCC
HincII 2068 GTCAAC GT[CT]^[GA]AC
2962 GTTAAC
HindII 2068 GTCAAC GT[CT]^[GA]AC
2962 GTTAAC
HindIII 3970 AAGCTT A^AGCTT
4115 AAGCTT
KasI 316 GGCGCC G^GCGCC
779 GGCGCC
MvnI 460 CGCG CG^CG
629 CGCG
NarI 316 GGCGCC GG^CGCC
779 GGCGCC
PvuII 4917 CAGCTG CAG^CTG
5047 CAGCTG
ScaI 1302 AGTACT AGT^ACT
2616 AGTACT
SfoI 316 GGCGCC GGC^GCC
779 GGCGCC
SspI 2997 AATATT AAT^ATT
4395 AATATT
TaqI 571 TCGA T^CGA
5186 TCGA
TthHB8I 571 TCGA T^CGA
5186 TCGA
XmnI 1073 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3115 GAAATTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspHI BspMI BsrDI
BsrGI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PstI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XhoII