##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccIII AhdI AlwNI AscI
AspEI AviII AvrII BamHI BcgI
BglI BlnI BsaBI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraIII DsaI EagI Eam1105I
EclXI Eco52I EcoRV EcoT22I FseI
FspI KspI MamI MfeI MluI
MroI MunI NcoI NdeI NheI
NotI NsiI NspV PacI PaeR7I
PmeI PvuI RsrII SacII SalI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SspBI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 4184 GGTACC G^GTACC
AccI 4114 GTAGAC GT^[AC][GT]AC
AccII 898 CGCG CG^CG
AclI 314 AACGTT AA^CGTT
AcyI 5556 GGCGCC G[GA]^CG[CT]C
AgeI 1001 ACCGGT A^CCGGT
Alw44I 839 GTGCAC G^TGCAC
AocI 4061 CCTTAGG CC^T[ACGT]AGG
Aor51HI 2915 AGCGCT AGC^GCT
ApaI 887 GGGCCC GGGCC^C
ApaLI 839 GTGCAC G^TGCAC
AseI 3957 ATTAAT AT^TAAT
AsnI 3957 ATTAAT AT^TAAT
Asp718I 4184 GGTACC G^GTACC
BbeI 5556 GGCGCC GGCGC^C
BcnI 2604 CCGGG CC^[GC]GG
BlpI 2248 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2248 GCTGAGC GC^T[ACGT]AGC
BsaHI 5556 GGCGCC G[GA]^CG[CT]C
BsaI 592 GGTCTC GGTCTC(1/5)
BsmBI 1966 GAGACG CGTCTC(1/5)
BspLU11I 829 ACATGT A^CATGT
BsrBI 3220 CCGCTC CCGCTC(-3/-3)
BstAPI 1351 GCAATTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4056 GGTAACC G^GT[ACGT]ACC
BstPI 4056 GGTAACC G^GT[ACGT]ACC
BstUI 898 CGCG CG^CG
Bsu36I 4061 CCTTAGG CC^T[ACGT]AGG
CelII 2248 GCTGAGC GC^T[ACGT]AGC
DrdI 1392 GACAACCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2915 AGCGCT AGC^GCT
Eco81I 4061 CCTTAGG CC^T[ACGT]AGG
EcoO65I 4056 GGTAACC G^GT[ACGT]ACC
HgaI 565 GACGC GACGC(5/10)
Hin1I 5556 GGCGCC G[GA]^CG[CT]C
HpaI 2800 GTTAAC GTT^AAC
KasI 5556 GGCGCC G^GCGCC
KpnI 4184 GGTACC GGTAC^C
MvnI 898 CGCG CG^CG
NaeI 3638 GCCGGC GCC^GGC
NarI 5556 GGCGCC GG^CGCC
NciI 2604 CCGGG CC^[GC]GG
NgoMIV 3638 GCCGGC G^CCGGC
NruI 897 TCGCGA TCG^CGA
PinAI 1001 ACCGGT A^CCGGT
PpuMI 332 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 2901 GACCACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3957 ATTAAT AT^TAAT
Psp1406I 314 AACGTT AA^CGTT
SbfI 2094 CCTGCAGG CCTGCA^GG
SfoI 5556 GGCGCC GGC^GCC
Sse8387I 2094 CCTGCAGG CCTGCA^GG
StuI 4652 AGGCCT AGG^CCT
XcmI 4418 CCACACTCAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AspI 571 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2739 GACTTCGTC
AvaI 5576 CTCGGG C^[CT]CG[GA]G
5608 CTCGGG
BbvCI 4637 CCTCAGC CCTCAGC(-5/-2)
2043 GCTGAGG
BciVI 4940 GTATCC GTATCC(6/5)
5334 GTATCC
BclI 3027 TGATCA T^GATCA
5074 TGATCA
BsaWI 1001 ACCGGT [AT]^CCGG[AT]
2860 ACCGGA
BsoBI 5576 CTCGGG C^[CT]CG[GA]G
5608 CTCGGG
BssSI 1693 CACGAG CACGAG(-5/-1)
3621 CACGAG
BstXI 1448 CCACCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5436 CCAGCACTTTGG
DraI 4078 TTTAAA TTT^AAA
4954 TTTAAA
DraII 332 AGGACCT [GA]G^G[ACGT]CC[CT]
452 AGGCCCT
EcoO109I 332 AGGACCT [GA]G^G[ACGT]CC[CT]
452 AGGCCCT
EcoRI 4014 GAATTC G^AATTC
4495 GAATTC
FbaI 3027 TGATCA T^GATCA
5074 TGATCA
HaeII 2915 AGCGCT [GA]GCGC^[CT]
5556 GGCGCC
PflFI 571 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2739 GACTTCGTC
SapI 427 GAAGAGC GCTCTTC(1/4)
3211 GAAGAGC
SphI 3152 GCATGC GCATG^C
3176 GCATGC
SspI 3377 AATATT AAT^ATT
4533 AATATT
Tth111I 571 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2739 GACTTCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI ApoI Asp700I AspHI
AvaII BalI BanI BanII BbrPI
BbsI BbvI BfaI BfrI BglII
BmyI BpmI BpuAI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII EaeI
EarI EcoNI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XhoII XmnI