##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BcnI BglI BlnI BseAI
BsiEI BsiWI BspEI BssHII BssSI
BstEII BstPI Bsu36I CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI FseI FspI HaeII HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NciI NgoMIV NotI NruI PaeR7I
PflFI PmaCI PmeI PmlI PpuMI
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SgrAI SmaI
SpeI SphI Sse8387I StuI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AclI 1892 AACGTT AA^CGTT
AcyI 1450 GGCGTC G[GA]^CG[CT]C
AgeI 4386 ACCGGT A^CCGGT
AlwNI 3353 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 5272 GAGCTC G[AT]GC[AT]^C
AvaI 1446 CTCGGG C^[CT]CG[GA]G
BalI 237 TGGCCA TGG^CCA
BciVI 4833 GGATAC GTATCC(6/5)
BclI 1272 TGATCA T^GATCA
BsaAI 4532 TACGTA [CT]AC^GT[GA]
BsaHI 1450 GGCGTC G[GA]^CG[CT]C
BsaWI 4386 ACCGGT [AT]^CCGG[AT]
BsiHKAI 5272 GAGCTC G[AT]GC[AT]^C
BsmBI 18 CGTCTC CGTCTC(1/5)
BsoBI 1446 CTCGGG C^[CT]CG[GA]G
Bsp1407I 3406 TGTACA T^GTACA
BspHI 1646 TCATGA T^CATGA
BsrBI 84 CCGCTC CCGCTC(-3/-3)
BsrFI 4386 ACCGGT [GA]^CCGG[CT]
BsrGI 3406 TGTACA T^GTACA
BstBI 1594 TTCGAA TT^CGAA
BstXI 2375 CCATTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 4386 ACCGGT [GA]^CCGG[CT]
EcoRV 2436 GATATC GAT^ATC
EcoT22I 4405 ATGCAT ATGCA^T
FbaI 1272 TGATCA T^GATCA
HapII 4387 CCGG C^CGG
Hin1I 1450 GGCGTC G[GA]^CG[CT]C
HpaII 4387 CCGG C^CGG
MluNI 237 TGGCCA TGG^CCA
MscI 237 TGGCCA TGG^CCA
MspI 4387 CCGG C^CGG
NdeI 4472 CATATG CA^TATG
NheI 3234 GCTAGC G^CTAGC
NsiI 4405 ATGCAT ATGCA^T
NspV 1594 TTCGAA TT^CGAA
PacI 1136 TTAATTAA TTAAT^TAA
PinAI 4386 ACCGGT A^CCGGT
Psp1406I 1892 AACGTT AA^CGTT
PvuII 4359 CAGCTG CAG^CTG
RcaI 1646 TCATGA T^CATGA
SacI 5272 GAGCTC GAGCT^C
SexAI 4880 ACCTGGT A^CC[AT]GGT
SfuI 1594 TTCGAA TT^CGAA
SnaBI 4532 TACGTA TAC^GTA
SspBI 3406 TGTACA T^GTACA
XbaI 686 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 27 CGCG CG^CG
55 CGCG
AflII 1737 CTTAAG C^TTAAG
5191 CTTAAG
AseI 1139 ATTAAT AT^TAAT
4656 ATTAAT
AsnI 1139 ATTAAT AT^TAAT
4656 ATTAAT
AvaII 141 GGACC G^G[AT]CC
1841 GGACC
BanII 4227 GAGCCC G[GA]GC[CT]^C
5272 GAGCTC
BfrI 1737 CTTAAG C^TTAAG
5191 CTTAAG
BglII 4365 AGATCT A^GATCT
4395 AGATCT
BlpI 997 GCTCAGC GC^T[ACGT]AGC
4311 GCTTAGC
BpmI 2309 CTGGAG CTGGAG(16/14)
4563 CTGGAG
Bpu1102I 997 GCTCAGC GC^T[ACGT]AGC
4311 GCTTAGC
BsaBI 1545 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3122 GATAAGTATC
BspDI 634 ATCGAT AT^CGAT
3959 ATCGAT
BspLU11I 75 ACATGT A^CATGT
5202 ACATGT
Bst1107I 3050 GTATAC GTA^TAC
3503 GTATAC
BstAPI 576 GCAAAGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3352 GCAGTAGCTGC
BstUI 27 CGCG CG^CG
55 CGCG
BstZ17I 3050 GTATAC GTA^TAC
3503 GTATAC
CelII 997 GCTCAGC GC^T[ACGT]AGC
4311 GCTTAGC
ClaI 634 ATCGAT AT^CGAT
3959 ATCGAT
EaeI 237 TGGCCA [CT]^GGCC[GA]
3498 TGGCCG
EcoNI 3267 CCTGCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3754 CCTAATGTAGG
HgaI 17 GCGTC GACGC(5/10)
1451 GCGTC
MamI 1545 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3122 GATAAGTATC
MvnI 27 CGCG CG^CG
55 CGCG
PshAI 121 GACGTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2307 GACTGGAGTC
PshBI 1139 ATTAAT AT^TAAT
4656 ATTAAT
SapI 1107 GAAGAGC GCTCTTC(1/4)
4434 GCTCTTC
SmlI 1737 CTTAAG C^T[CT][GA]AG
5191 CTTAAG
SwaI 2763 ATTTAAAT ATTT^AAAT
3525 ATTTAAAT
XcmI 368 CCACTCTTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4986 CCAGACCAATGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I BbsI
BbvI BfaI BmyI BpuAI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DsaI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HhaI HinP1I HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI