##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AscI Asp700I AviII AvrII BbeI
BbsI BlnI BpuAI BsaHI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BssHII ClaI CpoI DraIII DrdI
EagI EclXI Eco52I EcoRV EcoT22I
FseI FspI Hin1I HindIII HpaI
KasI MfeI MluI MroI MunI
NarI NdeI NotI NruI NsiI
PacI PaeR7I PinAI PmeI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfoI SnaBI
SpeI Sse8387I SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 1336 GGTACC G^GTACC
Aor51HI 1294 AGCGCT AGC^GCT
AseI 1128 ATTAAT AT^TAAT
AsnI 1128 ATTAAT AT^TAAT
Asp718I 1336 GGTACC G^GTACC
BciVI 5727 GGATAC GTATCC(6/5)
BsaBI 1113 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 74 TCCGGT [AT]^CCGG[AT]
BstBI 5560 TTCGAA TT^CGAA
BstEII 3872 GGTGACC G^GT[ACGT]ACC
BstPI 3872 GGTGACC G^GT[ACGT]ACC
Eco47III 1294 AGCGCT AGC^GCT
EcoNI 3507 CCTTCTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3872 GGTGACC G^GT[ACGT]ACC
EcoRI 4808 GAATTC G^AATTC
HincII 565 GTTGAC GT[CT]^[GA]AC
HindII 565 GTTGAC GT[CT]^[GA]AC
KpnI 1336 GGTACC GGTAC^C
KspI 93 CCGCGG CCGC^GG
MamI 1113 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 2920 CCATGG C^CATGG
NspV 5560 TTCGAA TT^CGAA
PshAI 1175 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1128 ATTAAT AT^TAAT
SacI 4343 GAGCTC GAGCT^C
SacII 93 CCGCGG CCGC^GG
SfiI 1382 GGCCAGAACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 5560 TTCGAA TT^CGAA
StuI 3724 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 3677 GTATAC GT^[AC][GT]AC
4271 GTATAC
AflII 3511 CTTAAG C^TTAAG
3542 CTTAAG
AhdI 754 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5603 GACAAAGAGTC
AocI 1456 CCTCAGG CC^T[ACGT]AGG
4072 CCTGAGG
ApaI 293 GGGCCC GGGCC^C
4381 GGGCCC
AspEI 754 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5603 GACAAAGAGTC
BamHI 71 GGATCC G^GATCC
5299 GGATCC
BbrPI 379 CACGTG CAC^GTG
2154 CACGTG
BcgI 5007 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
323 GCAGCAGCATCG
BfrI 3511 CTTAAG C^TTAAG
3542 CTTAAG
BglII 1946 AGATCT A^GATCT
5614 AGATCT
BsaAI 379 CACGTG [CT]AC^GT[GA]
2154 CACGTG
BsmBI 4922 GAGACG CGTCTC(1/5)
5089 GAGACG
BsmI 2226 GCATTC GAATGC(1/-1)
5747 GCATTC
Bsp1407I 3829 TGTACA T^GTACA
3839 TGTACA
BspLU11I 3279 ACATGT A^CATGT
3857 ACATGT
BsrDI 3437 GCAATG GCAATG(2/0)
4876 GCAATG
BsrGI 3829 TGTACA T^GTACA
3839 TGTACA
Bst1107I 3677 GTATAC GTA^TAC
4271 GTATAC
BstAPI 1362 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2629 GCATGAGCTGC
BstZ17I 3677 GTATAC GTA^TAC
4271 GTATAC
Bsu36I 1456 CCTCAGG CC^T[ACGT]AGG
4072 CCTGAGG
Eam1105I 754 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5603 GACAAAGAGTC
Eco81I 1456 CCTCAGG CC^T[ACGT]AGG
4072 CCTGAGG
HaeII 138 AGCGCC [GA]GCGC^[CT]
1294 AGCGCT
NaeI 141 GCCGGC GCC^GGC
796 GCCGGC
NgoMIV 141 GCCGGC G^CCGGC
796 GCCGGC
NheI 2857 GCTAGC G^CTAGC
5971 GCTAGC
PflMI 1802 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3016 CCAGGACATGG
PmaCI 379 CACGTG CAC^GTG
2154 CACGTG
PmlI 379 CACGTG CAC^GTG
2154 CACGTG
PstI 162 CTGCAG CTGCA^G
2185 CTGCAG
PvuII 1309 CAGCTG CAG^CTG
4065 CAGCTG
SgrAI 20 CACCGGCG C[GA]^CCGG[CT]G
140 CGCCGGCG
SphI 3179 GCATGC GCATG^C
5049 GCATGC
SspBI 3829 TGTACA T^GTACA
3839 TGTACA
Van91I 1802 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3016 CCAGGACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI AspI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BclI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XbaI XcmI XhoII
XmaCI XmaI