##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AatII AclI AgeI Aor51HI ApaI
AscI AviII AvrII BlnI BsaI
BsiEI BsiWI BsmBI BspDI BsrFI
BssHII Cfr10I ClaI CpoI DraIII
EagI EclXI Eco47III Eco52I FseI
FspI KspI MluI NaeI NgoMIV
NotI NruI PacI PinAI PmeI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SgrAI SmaI
SnaBI Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccII 8227 CGCG CG^CG
AccIII 1961 TCCGGA T^CCGGA
AcyI 4808 GGCGCC G[GA]^CG[CT]C
AhdI 5425 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 5425 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 8824 TGGCCA TGG^CCA
BbeI 4808 GGCGCC GGCGC^C
BbrPI 7827 CACGTG CAC^GTG
BbvCI 8314 GCTGAGG CCTCAGC(-5/-2)
BcgI 4854 CGACCTAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 8252 GCCCTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 4808 GGCGCC G[GA]^CG[CT]C
BsaWI 1961 TCCGGA [AT]^CCGG[AT]
BseAI 1961 TCCGGA T^CCGGA
BspEI 1961 TCCGGA T^CCGGA
BsrBI 5811 CCGCTC CCGCTC(-3/-3)
Bst1107I 8628 GTATAC GTA^TAC
BstBI 8422 TTCGAA TT^CGAA
BstUI 8227 CGCG CG^CG
BstZ17I 8628 GTATAC GTA^TAC
DrdI 3656 GACTACATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 8824 TGGCCA [CT]^GGCC[GA]
Eam1105I 5425 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 8166 GCGGG CCCGC(4/6)
HaeII 4808 GGCGCC [GA]GCGC^[CT]
Hin1I 4808 GGCGCC G[GA]^CG[CT]C
KasI 4808 GGCGCC G^GCGCC
MluNI 8824 TGGCCA TGG^CCA
MroI 1961 TCCGGA T^CCGGA
MscI 8824 TGGCCA TGG^CCA
MvnI 8227 CGCG CG^CG
NarI 4808 GGCGCC GG^CGCC
NheI 5926 GCTAGC G^CTAGC
NspV 8422 TTCGAA TT^CGAA
PmaCI 7827 CACGTG CAC^GTG
PmlI 7827 CACGTG CAC^GTG
SapI 3383 GAAGAGC GCTCTTC(1/4)
SfoI 4808 GGCGCC GGC^GCC
SfuI 8422 TTCGAA TT^CGAA
SspI 8446 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 3727 GGTACC G^GTACC
7245 GGTACC
AflII 3472 CTTAAG C^TTAAG
8507 CTTAAG
AocI 5800 CCTCAGG CC^T[ACGT]AGG
8672 CCTTAGG
AseI 5323 ATTAAT AT^TAAT
5460 ATTAAT
AsnI 5323 ATTAAT AT^TAAT
5460 ATTAAT
Asp718I 3727 GGTACC G^GTACC
7245 GGTACC
BamHI 4140 GGATCC G^GATCC
8265 GGATCC
BfrI 3472 CTTAAG C^TTAAG
8507 CTTAAG
BlpI 7918 GCTGAGC GC^T[ACGT]AGC
8218 GCTCAGC
Bpu1102I 7918 GCTGAGC GC^T[ACGT]AGC
8218 GCTCAGC
BsaAI 4465 CACGTA [CT]AC^GT[GA]
7827 CACGTG
BssSI 906 CACGAG CACGAG(-5/-1)
6590 CACGAG
BstEII 5806 GGTTACC G^GT[ACGT]ACC
6171 GGTGACC
BstPI 5806 GGTTACC G^GT[ACGT]ACC
6171 GGTGACC
BstXI 341 CCAGGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3052 CCATTATTGTGG
Bsu36I 5800 CCTCAGG CC^T[ACGT]AGG
8672 CCTTAGG
CelII 7918 GCTGAGC GC^T[ACGT]AGC
8218 GCTCAGC
CfoI 4809 GCGC GCG^C
5079 GCGC
Eco81I 5800 CCTCAGG CC^T[ACGT]AGG
8672 CCTTAGG
EcoO65I 5806 GGTTACC G^GT[ACGT]ACC
6171 GGTGACC
EcoRV 7633 GATATC GAT^ATC
8140 GATATC
HhaI 4809 GCGC GCG^C
5079 GCGC
HinP1I 4809 GCGC G^CGC
5079 GCGC
HpaI 2590 GTTAAC GTT^AAC
8740 GTTAAC
KpnI 3727 GGTACC GGTAC^C
7245 GGTACC
NdeI 2747 CATATG CA^TATG
3132 CATATG
PshBI 5323 ATTAAT AT^TAAT
5460 ATTAAT
SpeI 3085 ACTAGT A^CTAGT
3151 ACTAGT
SphI 1043 GCATGC GCATG^C
2485 GCATGC
StuI 3687 AGGCCT AGG^CCT
4166 AGGCCT
XcmI 3421 CCAGAGGAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
7843 CCAGAAGAGAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
ApoI Asp700I AspHI AspI AvaI
AvaII BanI BanII BbsI BbvI
BciVI BclI BcnI BfaI BglII
BmyI BpmI BpuAI BsaBI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspHI BspLU11I BspMI BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoNI EcoO109I EcoRI EcoRII EcoT14I
EcoT22I FbaI Fnu4HI FokI HaeIII
HapII HgaI HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MspI MunI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NsiI NspI PaeR7I PflFI
PflMI PleI PpuMI PshAI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XbaI XhoI
XhoII XmnI