##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp718I AspEI AspI AvrII
BbrPI BbvCI BcgI BlnI BlpI
Bpu1102I BsaAI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BspLU11I BsrBI
BssSI BstBI BstEII BstPI CelII
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FseI KpnI MluI MroI
NaeI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacI SapI SbfI SfuI SmaI
SnaBI Sse8387I StuI Tth111I XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcyI 157 GGCGCC G[GA]^CG[CT]C
AspHI 4665 GTGCTC G[AT]GC[AT]^C
AviII 3521 TGCGCA TGC^GCA
BamHI 3464 GGATCC G^GATCC
BbeI 157 GGCGCC GGCGC^C
BglII 969 AGATCT A^GATCT
BsaBI 4068 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 157 GGCGCC G[GA]^CG[CT]C
BsaI 2798 GAGACC GGTCTC(1/5)
BsiHKAI 4665 GTGCTC G[AT]GC[AT]^C
BsmBI 3699 GAGACG CGTCTC(1/5)
BspHI 773 TCATGA T^CATGA
BssHII 422 GCGCGC G^CGCGC
Bst1107I 5764 GTATAC GTA^TAC
BstAPI 3029 GCAGATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1810 CCATGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 5764 GTATAC GTA^TAC
DraIII 55 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 5154 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 3521 TGCGCA TGC^GCA
HgaI 203 GACGC GACGC(5/10)
Hin1I 157 GGCGCC G[GA]^CG[CT]C
HpaI 5987 GTTAAC GTT^AAC
KasI 157 GGCGCC G^GCGCC
KspI 74 CCGCGG CCGC^GG
MamI 4068 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 157 GGCGCC GG^CGCC
NcoI 2426 CCATGG C^CATGG
PaeR7I 207 CTCGAG C^TCGAG
PshAI 634 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 773 TCATGA T^CATGA
SacII 74 CCGCGG CCGC^GG
SalI 1937 GTCGAC G^TCGAC
SexAI 4098 ACCTGGT A^CC[AT]GGT
SfiI 3061 GGCCTGGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 157 GGCGCC GGC^GCC
SgrAI 4552 CACCGGCG C[GA]^CCGG[CT]G
SpeI 5453 ACTAGT A^CTAGT
SwaI 6056 ATTTAAAT ATTT^AAAT
XbaI 2687 TCTAGA T^CTAGA
XhoI 207 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1937 GTCGAC GT^[AC][GT]AC
5764 GTATAC
AccII 75 CGCG CG^CG
423 CGCG
AflII 723 CTTAAG C^TTAAG
5328 CTTAAG
AocI 293 CCTAAGG CC^T[ACGT]AGG
4986 CCTAAGG
AseI 2985 ATTAAT AT^TAAT
6108 ATTAAT
AsnI 2985 ATTAAT AT^TAAT
6108 ATTAAT
AvaI 207 CTCGAG C^[CT]CG[GA]G
378 CCCGAG
BclI 3107 TGATCA T^GATCA
6180 TGATCA
BfrI 723 CTTAAG C^TTAAG
5328 CTTAAG
BglI 38 GCCAGGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3062 GCCTGGATGGC
BseRI 1167 CTCCTC GAGGAG(10/8)
4758 CTCCTC
BsoBI 207 CTCGAG C^[CT]CG[GA]G
378 CCCGAG
Bsp1407I 560 TGTACA T^GTACA
5810 TGTACA
BsrFI 4553 ACCGGC [GA]^CCGG[CT]
5516 ACCGGC
BsrGI 560 TGTACA T^GTACA
5810 TGTACA
BstUI 75 CGCG CG^CG
423 CGCG
Bsu36I 293 CCTAAGG CC^T[ACGT]AGG
4986 CCTAAGG
Cfr10I 4553 ACCGGC [GA]^CCGG[CT]
5516 ACCGGC
DsaI 74 CCGCGG C^C[GA][CT]GG
2426 CCATGG
Eco81I 293 CCTAAGG CC^T[ACGT]AGG
4986 CCTAAGG
FbaI 3107 TGATCA T^GATCA
6180 TGATCA
HaeII 22 GGCGCT [GA]GCGC^[CT]
157 GGCGCC
MaeII 1089 ACGT A^CGT
3702 ACGT
MfeI 502 CAATTG C^AATTG
3514 CAATTG
MunI 502 CAATTG C^AATTG
3514 CAATTG
MvnI 75 CGCG CG^CG
423 CGCG
NdeI 6154 CATATG CA^TATG
6176 CATATG
PflMI 4448 CCAGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4485 CCACAGTATGG
PshBI 2985 ATTAAT AT^TAAT
6108 ATTAAT
SphI 1288 GCATGC GCATG^C
4799 GCATGC
SspBI 560 TGTACA T^GTACA
5810 TGTACA
Van91I 4448 CCAGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4485 CCACAGTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI Asp700I AvaII
BalI BanI BanII BbsI BbvI
BciVI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI