##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
Aor51HI AscI AseI AsnI Asp718I
AviII AvrII BcgI BfrI BlnI
BsiWI BsmI BspDI BssHII Bst1107I
BstZ17I ClaI CpoI DrdI Eco47III
EcoT22I FseI FspI HincII HindII
HpaI KpnI MfeI MunI NaeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PmeI PshAI PshBI
Psp1406I RsrII SalI SbfI SexAI
SnaBI SphI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 1736 TCCGGA T^CCGGA
AgeI 864 ACCGGT A^CCGGT
Alw44I 2644 GTGCAC G^TGCAC
ApaLI 2644 GTGCAC G^TGCAC
BanI 201 GGCGCC G^G[CT][GA]CC
BbeI 201 GGCGCC GGCGC^C
BclI 2197 TGATCA T^GATCA
BsaBI 3591 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1736 TCCGGA T^CCGGA
BspEI 1736 TCCGGA T^CCGGA
BspLU11I 1720 ACATGT A^CATGT
BstXI 4672 CCAGAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 5108 CGGCCG C^GGCCG
EclXI 5108 CGGCCG C^GGCCG
Eco52I 5108 CGGCCG C^GGCCG
EcoRV 3040 GATATC GAT^ATC
FbaI 2197 TGATCA T^GATCA
KasI 201 GGCGCC G^GCGCC
MamI 3591 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 4719 ACGCGT A^CGCGT
MroI 1736 TCCGGA T^CCGGA
NarI 201 GGCGCC GG^CGCC
NcoI 1811 CCATGG C^CATGG
NspI 1720 ACATGT [GA]CATG^[CT]
PinAI 864 ACCGGT A^CCGGT
PvuI 293 CGATCG CGAT^CG
ScaI 2785 AGTACT AGT^ACT
SfiI 808 GGCCTGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 201 GGCGCC GGC^GCC
SgrAI 863 CACCGGTG C[GA]^CCGG[CT]G
SpeI 5603 ACTAGT A^CTAGT
XcmI 4611 CCATCCCCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AocI 2689 CCTGAGG CC^T[ACGT]AGG
4247 CCTGAGG
AspI 2351 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3458 GACTGTGTC
BbrPI 1088 CACGTG CAC^GTG
4997 CACGTG
BbsI 798 GAAGAC GAAGAC(2/6)
3752 GTCTTC
BbvCI 4081 GCTGAGG CCTCAGC(-5/-2)
5172 GCTGAGG
BglI 809 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5181 GCCTGGGAGGC
BglII 1436 AGATCT A^GATCT
1886 AGATCT
BlpI 821 GCTCAGC GC^T[ACGT]AGC
4173 GCTTAGC
Bpu1102I 821 GCTCAGC GC^T[ACGT]AGC
4173 GCTTAGC
BpuAI 798 GAAGAC GAAGAC(2/6)
3752 GTCTTC
BsaAI 1088 CACGTG [CT]AC^GT[GA]
4997 CACGTG
BsgI 4603 CTGCAC GTGCAG(16/14)
4864 CTGCAC
BsmBI 4740 CGTCTC CGTCTC(1/5)
2307 GAGACG
Bsp1407I 5558 TGTACA T^GTACA
5660 TGTACA
BspHI 1333 TCATGA T^CATGA
3955 TCATGA
BsrBI 819 CCGCTC CCGCTC(-3/-3)
2370 CCGCTC
BsrDI 1500 CATTGC GCAATG(2/0)
3566 GCAATG
BsrGI 5558 TGTACA T^GTACA
5660 TGTACA
BstAPI 2646 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3492 GCAGCAACTGC
BstBI 943 TTCGAA TT^CGAA
3790 TTCGAA
BstEII 4701 GGTGACC G^GT[ACGT]ACC
5303 GGTCACC
BstPI 4701 GGTGACC G^GT[ACGT]ACC
5303 GGTCACC
Bsu36I 2689 CCTGAGG CC^T[ACGT]AGG
4247 CCTGAGG
CelII 821 GCTCAGC GC^T[ACGT]AGC
4173 GCTTAGC
DraIII 1776 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
2259 CACTGTGTG
Eco81I 2689 CCTGAGG CC^T[ACGT]AGG
4247 CCTGAGG
EcoO65I 4701 GGTGACC G^GT[ACGT]ACC
5303 GGTCACC
HindIII 3163 AAGCTT A^AGCTT
4954 AAGCTT
NspV 943 TTCGAA TT^CGAA
3790 TTCGAA
PflFI 2351 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3458 GACTGTGTC
PmaCI 1088 CACGTG CAC^GTG
4997 CACGTG
PmlI 1088 CACGTG CAC^GTG
4997 CACGTG
RcaI 1333 TCATGA T^CATGA
3955 TCATGA
SfuI 943 TTCGAA TT^CGAA
3790 TTCGAA
SspBI 5558 TGTACA T^GTACA
5660 TGTACA
SspI 1713 AATATT AAT^ATT
3473 AATATT
StuI 4844 AGGCCT AGG^CCT
5329 AGGCCT
Tth111I 2351 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3458 GACTGTGTC
XbaI 4089 TCTAGA T^CTAGA
4258 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
ApaI ApoI Asp700I AspEI AspHI
AvaI AvaII BalI BamHI BanII
BbvI BciVI BcnI BfaI BmyI
BpmI BsaHI BsaI BsaJI BsaWI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI Eam1105I EarI
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacI SacII
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI XmnI