##################################
# NO CUT ENZYMES ( 41 enzymes) #
##################################
Acc65I AccI AclI AgeI AscI
Asp718I AvrII BlnI BsiWI BsrBI
BssHII Bst1107I BstZ17I CpoI DrdI
FseI HpaI KpnI MfeI MluI
MunI NotI PacI PaeR7I PinAI
PmeI PshAI Psp1406I RsrII SalI
SbfI SfiI SmaI SnaBI Sse8387I
StuI SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 3632 GACGTC GACGT^C
AhdI 2092 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 5206 ATTAAT AT^TAAT
AsnI 5206 ATTAAT AT^TAAT
AspEI 2092 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 357 TGCGCA TGC^GCA
BamHI 4245 GGATCC G^GATCC
BbvCI 4654 CCTCAGC CCTCAGC(-5/-2)
BsaBI 565 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 4451 CGTCTC CGTCTC(1/5)
Bsp1407I 1238 TGTACA T^GTACA
BspDI 3951 ATCGAT AT^CGAT
BspHI 4109 TCATGA T^CATGA
BsrGI 1238 TGTACA T^GTACA
BssSI 1438 CACGAG CACGAG(-5/-1)
BstBI 1510 TTCGAA TT^CGAA
ClaI 3951 ATCGAT AT^CGAT
Eam1105I 2092 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2934 GAATTC G^AATTC
EcoRV 2722 GATATC GAT^ATC
EcoT22I 3333 ATGCAT ATGCA^T
FspI 357 TGCGCA TGC^GCA
MamI 565 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 3886 CCATGG C^CATGG
NheI 4039 GCTAGC G^CTAGC
NruI 35 TCGCGA TCG^CGA
NsiI 3333 ATGCAT ATGCA^T
NspV 1510 TTCGAA TT^CGAA
PshBI 5206 ATTAAT AT^TAAT
RcaI 4109 TCATGA T^CATGA
SacI 1768 GAGCTC GAGCT^C
SfuI 1510 TTCGAA TT^CGAA
SgrAI 171 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 6159 ACTAGT A^CTAGT
SspBI 1238 TGTACA T^GTACA
SspI 5241 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Aor51HI 338 AGCGCT AGC^GCT
859 AGCGCT
ApaI 194 GGGCCC GGGCC^C
4905 GGGCCC
Asp700I 1503 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4322 GAAATCTTTC
BbeI 1783 GGCGCC GGCGC^C
2515 GGCGCC
BbrPI 2665 CACGTG CAC^GTG
4915 CACGTG
BcgI 584 CGATGACAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5735 CGATTGTTCTGC
BciVI 5686 GGATAC GTATCC(6/5)
3345 GTATCC
BlpI 2833 GCTGAGC GC^T[ACGT]AGC
4281 GCTAAGC
Bpu1102I 2833 GCTGAGC GC^T[ACGT]AGC
4281 GCTAAGC
BsaAI 2665 CACGTG [CT]AC^GT[GA]
4915 CACGTG
BsaI 2006 GAGACC GGTCTC(1/5)
31 GGTCTC
BspLU11I 1356 ACATGT A^CATGT
2282 ACATGT
BsrDI 5787 CATTGC GCAATG(2/0)
5863 GCAATG
BstEII 3786 GGTCACC G^GT[ACGT]ACC
5623 GGTTACC
BstPI 3786 GGTCACC G^GT[ACGT]ACC
5623 GGTTACC
CelII 2833 GCTGAGC GC^T[ACGT]AGC
4281 GCTAAGC
EagI 138 CGGCCG C^GGCCG
3189 CGGCCG
EclXI 138 CGGCCG C^GGCCG
3189 CGGCCG
Eco47III 338 AGCGCT AGC^GCT
859 AGCGCT
Eco52I 138 CGGCCG C^GGCCG
3189 CGGCCG
EcoNI 831 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1299 CCTTCTGCAGG
EcoO65I 3786 GGTCACC G^GT[ACGT]ACC
5623 GGTTACC
HindIII 4382 AAGCTT A^AGCTT
5420 AAGCTT
KasI 1783 GGCGCC G^GCGCC
2515 GGCGCC
NarI 1783 GGCGCC GG^CGCC
2515 GGCGCC
NdeI 2262 CATATG CA^TATG
3919 CATATG
PflMI 2061 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2384 CCACATGCTGG
PmaCI 2665 CACGTG CAC^GTG
4915 CACGTG
PmlI 2665 CACGTG CAC^GTG
4915 CACGTG
PvuI 1892 CGATCG CGAT^CG
2051 CGATCG
SapI 1948 GCTCTTC GCTCTTC(1/4)
3166 GCTCTTC
ScaI 5361 AGTACT AGT^ACT
6156 AGTACT
SexAI 2403 ACCTGGT A^CC[AT]GGT
3491 ACCAGGT
SfoI 1783 GGCGCC GGC^GCC
2515 GGCGCC
Van91I 2061 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2384 CCACATGCTGG
XcmI 542 CCACTAAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5876 CCAGCTATGGACTGG
XmnI 1503 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4322 GAAATCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AflII AflIII AluI Alw44I
AlwI AlwNI AocI ApaLI ApoI
AspHI AspI AvaI AvaII BalI
BanI BanII BbsI BbvI BclI
BcnI BfaI BfrI BglI BglII
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspEI BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflFI
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XhoII