##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AcyI AgeI AhdI
AocI AscI Asp718I AspEI BamHI
BbeI BcgI BglII BsaHI BsiEI
BsiWI Bsp1407I BspDI BsrGI Bst1107I
BstBI BstZ17I Bsu36I ClaI DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoRI FseI Hin1I HpaI KasI
KpnI KspI MluI NarI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PvuI
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SnaBI SphI Sse8387I
SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 1850 GTAGAC GT^[AC][GT]AC
AccIII 2067 TCCGGA T^CCGGA
AclI 3824 AACGTT AA^CGTT
Aor51HI 2242 AGCGCT AGC^GCT
AviII 1578 TGCGCA TGC^GCA
AvrII 3235 CCTAGG C^CTAGG
BbvCI 5770 GCTGAGG CCTCAGC(-5/-2)
BglI 2274 GCCCGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3235 CCTAGG C^CTAGG
BlpI 1981 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1981 GCTGAGC GC^T[ACGT]AGC
BseAI 2067 TCCGGA T^CCGGA
BsmBI 2521 GAGACG CGTCTC(1/5)
BspEI 2067 TCCGGA T^CCGGA
BspHI 1682 TCATGA T^CATGA
BsrFI 132 GCCGGC [GA]^CCGG[CT]
BssHII 2888 GCGCGC G^CGCGC
BstAPI 2283 GCAGTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1981 GCTGAGC GC^T[ACGT]AGC
Cfr10I 132 GCCGGC [GA]^CCGG[CT]
CpoI 860 CGGTCCG CG^G[AT]CCG
Eco47III 2242 AGCGCT AGC^GCT
EcoNI 4971 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1578 TGCGCA TGC^GCA
HincII 101 GTCAAC GT[CT]^[GA]AC
HindII 101 GTCAAC GT[CT]^[GA]AC
MfeI 1067 CAATTG C^AATTG
MroI 2067 TCCGGA T^CCGGA
MunI 1067 CAATTG C^AATTG
NaeI 132 GCCGGC GCC^GGC
NgoMIV 132 GCCGGC G^CCGGC
PflMI 1734 CCACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 3824 AACGTT AA^CGTT
RcaI 1682 TCATGA T^CATGA
RsrII 860 CGGTCCG CG^G[AT]CCG
SapI 31 GCTCTTC GCTCTTC(1/4)
SpeI 318 ACTAGT A^CTAGT
StuI 3850 AGGCCT AGG^CCT
Van91I 1734 CCACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3574 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AseI 1078 ATTAAT AT^TAAT
1458 ATTAAT
AsnI 1078 ATTAAT AT^TAAT
1458 ATTAAT
AspI 2038 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5861 GACTCTGTC
BanI 2299 GGCACC G^G[CT][GA]CC
4258 GGTGCC
BciVI 2548 GGATAC GTATCC(6/5)
5561 GGATAC
BsaBI 1674 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5051 GATTCTGATC
BsaWI 2067 TCCGGA [AT]^CCGG[AT]
2488 ACCGGA
BstEII 2511 GGTTACC G^GT[ACGT]ACC
4650 GGTCACC
BstPI 2511 GGTTACC G^GT[ACGT]ACC
4650 GGTCACC
BstYI 1424 GGATCT [GA]^GATC[CT]
4994 AGATCC
EcoO65I 2511 GGTTACC G^GT[ACGT]ACC
4650 GGTCACC
EcoT22I 378 ATGCAT ATGCA^T
3831 ATGCAT
HaeII 697 GGCGCT [GA]GCGC^[CT]
2242 AGCGCT
HgaI 3489 GACGC GACGC(5/10)
2042 GCGTC
HindIII 1539 AAGCTT A^AGCTT
5068 AAGCTT
MamI 1674 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5051 GATTCTGATC
MflI 1424 GGATCT [GA]^GATC[CT]
4994 AGATCC
NsiI 378 ATGCAT ATGCA^T
3831 ATGCAT
PflFI 2038 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5861 GACTCTGTC
PshBI 1078 ATTAAT AT^TAAT
1458 ATTAAT
SmaI 309 CCCGGG CCC^GGG
4999 CCCGGG
SspI 1862 AATATT AAT^ATT
3452 AATATT
Tth111I 2038 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5861 GACTCTGTC
XcmI 630 CCACATGGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
828 CCACACCCTTGATGG
XhoII 1424 GGATCT [GA]^GATC[CT]
4994 AGATCC
XmaCI 309 CCCGGG C^CCGGG
4999 CCCGGG
XmaI 309 CCCGGG C^CCGGG
4999 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI Asp700I AspHI
AvaI AvaII BalI BanII BbrPI
BbsI BbvI BclI BcnI BfaI
BfrI BmyI BpmI BpuAI BsaAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspLU11I BspMI BsrBI
BsrDI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRII EcoRV EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScaI ScrFI SexAI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI