##################################
# NO CUT ENZYMES (   52 enzymes) #
##################################

Acc65I      AccIII      AclI        AscI        Asp718I     
AviII       BcgI        BseAI       BsiWI       BsmBI       
BspDI       BspEI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
EagI        EclXI       Eco52I      EcoO65I     FseI        
FspI        HindIII     KpnI        KspI        MfeI        
MluI        MroI        MunI        NdeI        NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PmeI        Psp1406I    SacII       SalI        SbfI        
SfiI        SfuI        SgrAI       SnaBI       Sse8387I    
SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AgeI         1875  ACCGGT               A^CCGGT
AhdI         2558  GACACCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       5105  GTGCAC               G^TGCAC
ApaLI        5105  GTGCAC               G^TGCAC
AspEI        2558  GACACCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI        5716  GGATCC               G^GATCC
BbeI         1059  GGCGCC               GGCGC^C
BsiEI        4915  CGATCG               CG[GA][CT]^CG
BspLU11I     8081  ACATGT               A^CATGT
BspMI        9703  ACCTGC               ACCTGC(4/8)
CpoI         6561  CGGACCG              CG^G[AT]CCG
DraIII       8138  CACATTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         6388  GACTGGGGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     2558  GACACCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HpaI         2738  GTTAAC               GTT^AAC
KasI         1059  GGCGCC               G^GCGCC
NaeI         4340  GCCGGC               GCC^GGC
NarI         1059  GGCGCC               GG^CGCC
NgoMIV       4340  GCCGGC               G^CCGGC
PinAI        1875  ACCGGT               A^CCGGT
PshAI         689  GACCACGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI         4915  CGATCG               CGAT^CG
RsrII        6561  CGGACCG              CG^G[AT]CCG
SapI         4987  GAAGAGC              GCTCTTC(1/4)
SfoI         1059  GGCGCC               GGC^GCC
SmaI         6198  CCCGGG               CCC^GGG
SspI         8148  AATATT               AAT^ATT
XmaCI        6198  CCCGGG               C^CCGGG
XmaI         6198  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AatII         407  GACGTC               GACGT^C
             5039  GACGTC              
AccI         1973  GTAGAC               GT^[AC][GT]AC
             3707  GTCTAC              
AocI         6629  CCTGAGG              CC^T[ACGT]AGG
             8541  CCTAAGG             
AseI         3416  ATTAAT               AT^TAAT
             9843  ATTAAT              
AsnI         3416  ATTAAT               AT^TAAT
             9843  ATTAAT              
AvrII        5398  CCTAGG               C^CTAGG
             7655  CCTAGG              
BbrPI         866  CACGTG               CAC^GTG
             5108  CACGTG              
BlnI         5398  CCTAGG               C^CTAGG
             7655  CCTAGG              
Bsp1407I     1029  TGTACA               T^GTACA
             6956  TGTACA              
BsrBI        3567  CCGCTC               CCGCTC(-3/-3)
             3514  GAGCGG              
BsrFI        1875  ACCGGT               [GA]^CCGG[CT]
             4340  GCCGGC              
BsrGI        1029  TGTACA               T^GTACA
             6956  TGTACA              
BstAPI       1694  GCAGTGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3404  GCAGTCATTGC         
Bsu36I       6629  CCTGAGG              CC^T[ACGT]AGG
             8541  CCTAAGG             
Cfr10I       1875  ACCGGT               [GA]^CCGG[CT]
             4340  GCCGGC              
Eco81I       6629  CCTGAGG              CC^T[ACGT]AGG
             8541  CCTAAGG             
EcoRI        2660  GAATTC               G^AATTC
             5306  GAATTC              
EcoRV         779  GATATC               GAT^ATC
             1820  GATATC              
PmaCI         866  CACGTG               CAC^GTG
             5108  CACGTG              
PmlI          866  CACGTG               CAC^GTG
             5108  CACGTG              
PshBI        3416  ATTAAT               AT^TAAT
             9843  ATTAAT              
SexAI        1444  ACCAGGT              A^CC[AT]GGT
             6246  ACCTGGT             
SspBI        1029  TGTACA               T^GTACA
             6956  TGTACA              
StuI          940  AGGCCT               AGG^CCT
             9577  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  153 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflII       AflIII      AluI        AlwI        AlwNI       
Aor51HI     ApaI        ApoI        Asp700I     AspHI       
AspI        AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvCI       BbvI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspHI       BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EaeI        EarI        Eco47III    EcoNI       EcoO109I    
EcoRII      EcoT14I     EcoT22I     FauI        FbaI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MamI        
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NsiI        NspI        
PflFI       PflMI       PleI        PpuMI       PstI        
PvuII       RcaI        RsaI        SacI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SmlI        SpeI        SphI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoII       XmnI