##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Aor51HI AscI AspEI AvrII BbrPI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiWI BsmBI BspEI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CelII CpoI Eam1105I Eco47III
EcoO65I FseI HpaI MamI MluI
MroI NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SalI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 2831 GGTACC G^GTACC
AflII 4696 CTTAAG C^TTAAG
AflIII 1275 ACATGT A^C[GA][CT]GT
Alw44I 1279 GTGCAC G^TGCAC
ApaLI 1279 GTGCAC G^TGCAC
AseI 2666 ATTAAT AT^TAAT
AsnI 2666 ATTAAT AT^TAAT
Asp700I 3475 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2831 GGTACC G^GTACC
AviII 1354 TGCGCA TGC^GCA
BalI 5381 TGGCCA TGG^CCA
BbvCI 193 GCTGAGG CCTCAGC(-5/-2)
BcgI 4998 GCACCAAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3360 TGATCA T^GATCA
BfrI 4696 CTTAAG C^TTAAG
BglII 5483 AGATCT A^GATCT
Bsp1407I 938 TGTACA T^GTACA
BspHI 1300 TCATGA T^CATGA
BspLU11I 1275 ACATGT A^CATGT
BsrGI 938 TGTACA T^GTACA
BssSI 4521 CACGAG CACGAG(-5/-1)
DrdI 5099 GACAAAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1964 GAATTC G^AATTC
EcoRV 432 GATATC GAT^ATC
FbaI 3360 TGATCA T^GATCA
FspI 1354 TGCGCA TGC^GCA
KpnI 2831 GGTACC GGTAC^C
KspI 3292 CCGCGG CCGC^GG
MfeI 4686 CAATTG C^AATTG
MluNI 5381 TGGCCA TGG^CCA
MscI 5381 TGGCCA TGG^CCA
MunI 4686 CAATTG C^AATTG
PaeR7I 3210 CTCGAG C^TCGAG
PshAI 5458 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2666 ATTAAT AT^TAAT
RcaI 1300 TCATGA T^CATGA
SacI 2723 GAGCTC GAGCT^C
SacII 3292 CCGCGG CCGC^GG
SphI 539 GCATGC GCATG^C
SspBI 938 TGTACA T^GTACA
SspI 5571 AATATT AAT^ATT
XbaI 5729 TCTAGA T^CTAGA
XhoI 3210 CTCGAG C^TCGAG
XmnI 3475 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 351 GTAGAC GT^[AC][GT]AC
786 GTAGAC
AcsI 1964 GAATTC [GA]^AATT[CT]
5076 AAATTT
ApoI 1964 GAATTC [GA]^AATT[CT]
5076 AAATTT
AspI 2915 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3533 GACAAAGTC
BamHI 1108 GGATCC G^GATCC
1891 GGATCC
BbeI 1053 GGCGCC GGCGC^C
4729 GGCGCC
BciVI 4112 GGATAC GTATCC(6/5)
2471 GTATCC
BsiEI 32 CGGCCG CG[GA][CT]^CG
2826 CGGCCG
BspDI 849 ATCGAT AT^CGAT
3365 ATCGAT
BsrDI 4209 CATTGC GCAATG(2/0)
901 GCAATG
ClaI 849 ATCGAT AT^CGAT
3365 ATCGAT
EagI 32 CGGCCG C^GGCCG
2826 CGGCCG
EclXI 32 CGGCCG C^GGCCG
2826 CGGCCG
Eco52I 32 CGGCCG C^GGCCG
2826 CGGCCG
EcoT22I 541 ATGCAT ATGCA^T
4814 ATGCAT
HincII 1919 GTCAAC GT[CT]^[GA]AC
4445 GTTGAC
HindII 1919 GTCAAC GT[CT]^[GA]AC
4445 GTTGAC
HindIII 3156 AAGCTT A^AGCTT
4699 AAGCTT
KasI 1053 GGCGCC G^GCGCC
4729 GGCGCC
MaeII 2259 ACGT A^CGT
3432 ACGT
MslI 4586 CACATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4715 CACCTGGATG
NaeI 265 GCCGGC GCC^GGC
3784 GCCGGC
NarI 1053 GGCGCC GG^CGCC
4729 GGCGCC
NcoI 1824 CCATGG C^CATGG
2941 CCATGG
NdeI 322 CATATG CA^TATG
5286 CATATG
NgoMIV 265 GCCGGC G^CCGGC
3784 GCCGGC
NsiI 541 ATGCAT ATGCA^T
4814 ATGCAT
NspI 539 GCATGC [GA]CATG^[CT]
1275 ACATGT
PflFI 2915 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3533 GACAAAGTC
SexAI 1388 ACCAGGT A^CC[AT]GGT
2340 ACCTGGT
SfoI 1053 GGCGCC GGC^GCC
4729 GGCGCC
Tth111I 2915 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3533 GACAAAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI