##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspI AviII
BanI BbeI BbrPI BciVI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspLU11I BsrBI BssHII BstBI
BstUI ClaI CpoI DraIII EagI
EclXI Eco47III Eco52I FseI FspI
HaeII KasI KpnI KspI MluI
MroI MvnI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SexAI SfoI SfuI SgrAI SnaBI
SphI Sse8387I StuI SwaI Tth111I
Van91I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 1889 GACGTC GACGT^C
AccI 1469 GTATAC GT^[AC][GT]AC
AcyI 1889 GACGTC G[GA]^CG[CT]C
AflIII 2763 ACACGT A^C[GA][CT]GT
AocI 4283 CCTTAGG CC^T[ACGT]AGG
AvaI 3157 CCCGGG C^[CT]CG[GA]G
AvrII 2020 CCTAGG C^CTAGG
BamHI 896 GGATCC G^GATCC
BbsI 179 GTCTTC GAAGAC(2/6)
BbvCI 1259 GCTGAGG CCTCAGC(-5/-2)
BcgI 5423 CGAGGTGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 2020 CCTAGG C^CTAGG
BlpI 2980 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2980 GCTCAGC GC^T[ACGT]AGC
BpuAI 179 GTCTTC GAAGAC(2/6)
BsaAI 4661 TACGTG [CT]AC^GT[GA]
BsaHI 1889 GACGTC G[GA]^CG[CT]C
BsoBI 3157 CCCGGG C^[CT]CG[GA]G
BsrFI 1341 ACCGGC [GA]^CCGG[CT]
BssSI 2073 CACGAG CACGAG(-5/-1)
Bst1107I 1469 GTATAC GTA^TAC
BstAPI 5173 GCAGGAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1469 GTATAC GTA^TAC
Bsu36I 4283 CCTTAGG CC^T[ACGT]AGG
CelII 2980 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1341 ACCGGC [GA]^CCGG[CT]
DrdI 1883 GACAAAGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 4420 CCATGG C^C[GA][CT]GG
Eco81I 4283 CCTTAGG CC^T[ACGT]AGG
EcoRV 4782 GATATC GAT^ATC
Hin1I 1889 GACGTC G[GA]^CG[CT]C
HincII 3908 GTTAAC GT[CT]^[GA]AC
HindII 3908 GTTAAC GT[CT]^[GA]AC
HpaI 3908 GTTAAC GTT^AAC
MfeI 4063 CAATTG C^AATTG
MunI 4063 CAATTG C^AATTG
NcoI 4420 CCATGG C^CATGG
NdeI 393 CATATG CA^TATG
PshAI 2546 GACAGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 3019 GAAGAGC GCTCTTC(1/4)
SfiI 1320 GGCCCCATAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 3157 CCCGGG CCC^GGG
SpeI 407 ACTAGT A^CTAGT
XmaCI 3157 CCCGGG C^CCGGG
XmaI 3157 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AflII 4550 CTTAAG C^TTAAG
5239 CTTAAG
AhdI 568 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1340 GACCGGCTGTC
AspEI 568 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1340 GACCGGCTGTC
AspHI 1914 GTGCTC G[AT]GC[AT]^C
4411 GTGCTC
BalI 1785 TGGCCA TGG^CCA
4384 TGGCCA
BanII 206 GGGCTC G[GA]GC[CT]^C
4811 GAGCCC
BcnI 3157 CCCGG CC^[GC]GG
3158 CCGGG
BfrI 4550 CTTAAG C^TTAAG
5239 CTTAAG
BglI 1321 GCCCCATAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4350 GCCTGTCTGGC
BglII 501 AGATCT A^GATCT
1972 AGATCT
BsaBI 2921 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3562 GATCAGGATC
BsaI 453 GAGACC GGTCTC(1/5)
1076 GAGACC
BsiHKAI 1914 GTGCTC G[AT]GC[AT]^C
4411 GTGCTC
Bsp1407I 1945 TGTACA T^GTACA
4182 TGTACA
BspHI 2410 TCATGA T^CATGA
2440 TCATGA
BsrGI 1945 TGTACA T^GTACA
4182 TGTACA
BstEII 3553 GGTTACC G^GT[ACGT]ACC
5000 GGTAACC
BstPI 3553 GGTTACC G^GT[ACGT]ACC
5000 GGTAACC
CfoI 1153 GCGC GCG^C
4331 GCGC
DraI 3393 TTTAAA TTT^AAA
3725 TTTAAA
DraII 683 AGGACCT [GA]G^G[ACGT]CC[CT]
4239 AGGTCCT
Eam1105I 568 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1340 GACCGGCTGTC
EarI 3019 GAAGAG CTCTTC(1/4)
5096 GAAGAG
EcoO109I 683 AGGACCT [GA]G^G[ACGT]CC[CT]
4239 AGGTCCT
EcoO65I 3553 GGTTACC G^GT[ACGT]ACC
5000 GGTAACC
EcoRI 398 GAATTC G^AATTC
2629 GAATTC
FauI 2287 GCGGG CCCGC(4/6)
5297 GCGGG
HgaI 756 GACGC GACGC(5/10)
708 GCGTC
HhaI 1153 GCGC GCG^C
4331 GCGC
HinP1I 1153 GCGC G^CGC
4331 GCGC
HindIII 540 AAGCTT A^AGCTT
3533 AAGCTT
Ksp632I 3019 GAAGAG CTCTTC(1/4)
5096 GAAGAG
MamI 2921 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3562 GATCAGGATC
MluNI 1785 TGGCCA TGG^CCA
4384 TGGCCA
MscI 1785 TGGCCA TGG^CCA
4384 TGGCCA
NciI 3157 CCCGG CC^[GC]GG
3158 CCGGG
PpuMI 683 AGGACCT [GA]G^G[AT]CC[CT]
4239 AGGTCCT
PstI 311 CTGCAG CTGCA^G
2604 CTGCAG
PvuII 188 CAGCTG CAG^CTG
3150 CAGCTG
RcaI 2410 TCATGA T^CATGA
2440 TCATGA
SspBI 1945 TGTACA T^GTACA
4182 TGTACA
XbaI 622 TCTAGA T^CTAGA
3511 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AvaII BbvI
BclI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
EaeI EcoNI EcoRII EcoT14I EcoT22I
FbaI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI PleI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI