##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Aor51HI AscI Asp718I BfrI
BsaWI BseAI BsiWI BspDI BspEI
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI Eco47III FseI HindIII HpaI
KpnI MfeI MroI MunI NaeI
NdeI NgoMIV NspV PacI PinAI
PmeI PshAI Psp1406I PvuI RsrII
SalI SbfI SfiI SfuI SgrAI
SnaBI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AocI 4545 CCTTAGG CC^T[ACGT]AGG
AviII 1163 TGCGCA TGC^GCA
BalI 2602 TGGCCA TGG^CCA
BbrPI 5711 CACGTG CAC^GTG
BciVI 389 GTATCC GTATCC(6/5)
BglII 577 AGATCT A^GATCT
BlpI 2002 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2002 GCTGAGC GC^T[ACGT]AGC
BsaAI 5711 CACGTG [CT]AC^GT[GA]
Bsp1407I 2810 TGTACA T^GTACA
BspLU11I 293 ACATGT A^CATGT
BsrGI 2810 TGTACA T^GTACA
BssHII 13 GCGCGC G^CGCGC
BssSI 3296 CACGAG CACGAG(-5/-1)
BstEII 1287 GGTGACC G^GT[ACGT]ACC
BstPI 1287 GGTGACC G^GT[ACGT]ACC
Bsu36I 4545 CCTTAGG CC^T[ACGT]AGG
CelII 2002 GCTGAGC GC^T[ACGT]AGC
DraIII 5505 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 4545 CCTTAGG CC^T[ACGT]AGG
EcoO65I 1287 GGTGACC G^GT[ACGT]ACC
EcoRV 3859 GATATC GAT^ATC
EcoT22I 5360 ATGCAT ATGCA^T
FspI 1163 TGCGCA TGC^GCA
HincII 3333 GTCAAC GT[CT]^[GA]AC
HindII 3333 GTCAAC GT[CT]^[GA]AC
MluI 4872 ACGCGT A^CGCGT
MluNI 2602 TGGCCA TGG^CCA
MscI 2602 TGGCCA TGG^CCA
NheI 3756 GCTAGC G^CTAGC
NotI 5960 GCGGCCGC GC^GGCCGC
NruI 766 TCGCGA TCG^CGA
NsiI 5360 ATGCAT ATGCA^T
PflMI 653 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 5711 CACGTG CAC^GTG
PmlI 5711 CACGTG CAC^GTG
SexAI 3789 ACCAGGT A^CC[AT]GGT
SpeI 3521 ACTAGT A^CTAGT
SspBI 2810 TGTACA T^GTACA
SspI 2087 AATATT AAT^ATT
Van91I 653 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3814 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccI 3785 GTAGAC GT^[AC][GT]AC
4876 GTCTAC
AcyI 5012 GGCGCC G[GA]^CG[CT]C
5267 GGCGCC
AhdI 1071 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3705 GACAGGGAGTC
AseI 2483 ATTAAT AT^TAAT
2988 ATTAAT
AsnI 2483 ATTAAT AT^TAAT
2988 ATTAAT
AspEI 1071 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3705 GACAGGGAGTC
AspI 2792 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3380 GACTCAGTC
AvrII 1609 CCTAGG C^CTAGG
3824 CCTAGG
BamHI 880 GGATCC G^GATCC
4671 GGATCC
BbeI 5012 GGCGCC GGCGC^C
5267 GGCGCC
BglI 2007 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5809 GCCTGTGCGGC
BlnI 1609 CCTAGG C^CTAGG
3824 CCTAGG
BsaHI 5012 GGCGCC G[GA]^CG[CT]C
5267 GGCGCC
BsaI 4556 GAGACC GGTCTC(1/5)
5792 GGTCTC
BsiEI 1435 CGGCCG CG[GA][CT]^CG
5961 CGGCCG
BsmBI 1600 CGTCTC CGTCTC(1/5)
5525 CGTCTC
BsmI 5451 GAATGC GAATGC(1/-1)
1847 GCATTC
BspHI 2045 TCATGA T^CATGA
3938 TCATGA
DraI 2383 TTTAAA TTT^AAA
2411 TTTAAA
EagI 1435 CGGCCG C^GGCCG
5961 CGGCCG
Eam1105I 1071 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3705 GACAGGGAGTC
EclXI 1435 CGGCCG C^GGCCG
5961 CGGCCG
Eco52I 1435 CGGCCG C^GGCCG
5961 CGGCCG
EcoNI 806 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1460 CCTCCCAGAGG
EcoRI 2061 GAATTC G^AATTC
3899 GAATTC
HaeII 5012 GGCGCC [GA]GCGC^[CT]
5267 GGCGCC
Hin1I 5012 GGCGCC G[GA]^CG[CT]C
5267 GGCGCC
KasI 5012 GGCGCC G^GCGCC
5267 GGCGCC
KspI 5306 CCGCGG CCGC^GG
5970 CCGCGG
NarI 5012 GGCGCC GG^CGCC
5267 GGCGCC
PaeR7I 1561 CTCGAG C^TCGAG
5315 CTCGAG
PflFI 2792 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3380 GACTCAGTC
PshBI 2483 ATTAAT AT^TAAT
2988 ATTAAT
RcaI 2045 TCATGA T^CATGA
3938 TCATGA
SacII 5306 CCGCGG CCGC^GG
5970 CCGCGG
ScaI 3963 AGTACT AGT^ACT
4348 AGTACT
SfoI 5012 GGCGCC GGC^GCC
5267 GGCGCC
SphI 2672 GCATGC GCATG^C
4226 GCATGC
StuI 1457 AGGCCT AGG^CCT
3821 AGGCCT
Tth111I 2792 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3380 GACTCAGTC
XhoI 1561 CTCGAG C^TCGAG
5315 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcgI BclI BcnI BfaI
BmyI BpmI BpuAI BsaBI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI XmnI