##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
Alw44I AocI ApaI ApaLI AscI
Asp718I AvrII BalI BamHI BanI
BbeI BcgI BcnI BglII BlnI
BsaBI BsaHI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspLU11I BsrFI BsrGI
BssHII BssSI BstBI BstEII BstPI
Bsu36I Cfr10I ClaI CpoI DrdI
DsaI EaeI EagI EclXI Eco52I
Eco81I EcoO65I EcoRI EcoRV FauI
FseI Hin1I KasI KpnI KspI
MamI MfeI MluI MluNI MscI
MunI NaeI NarI NciI NcoI
NgoMIV NotI NruI NspV PacI
PaeR7I PflMI PinAI PmeI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
StuI SwaI Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccII 96 CGCG CG^CG
AccIII 1816 TCCGGA T^CCGGA
AflII 2336 CTTAAG C^TTAAG
AflIII 2923 ACACGT A^C[GA][CT]GT
AhdI 2443 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2661 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 447 AGCGCT AGC^GCT
AseI 3207 ATTAAT AT^TAAT
AsnI 3207 ATTAAT AT^TAAT
AspEI 2443 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 181 CTCGGG C^[CT]CG[GA]G
AviII 1898 TGCGCA TGC^GCA
BfrI 2336 CTTAAG C^TTAAG
BlpI 1436 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1436 GCTCAGC GC^T[ACGT]AGC
BseAI 1816 TCCGGA T^CCGGA
BseRI 984 CTCCTC GAGGAG(10/8)
BsoBI 181 CTCGGG C^[CT]CG[GA]G
BspEI 1816 TCCGGA T^CCGGA
BspHI 2833 TCATGA T^CATGA
BspMI 835 GCAGGT ACCTGC(4/8)
BsrBI 733 GAGCGG CCGCTC(-3/-3)
BsrDI 468 GCAATG GCAATG(2/0)
BstAPI 1556 GCAGATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 96 CGCG CG^CG
CelII 1436 GCTCAGC GC^T[ACGT]AGC
DraIII 1116 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2443 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 447 AGCGCT AGC^GCT
EcoNI 2518 CCTTGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1662 CCAAGG C^C[AT][AT]GG
EcoT22I 858 ATGCAT ATGCA^T
FspI 1898 TGCGCA TGC^GCA
HgaI 97 GCGTC GACGC(5/10)
MroI 1816 TCCGGA T^CCGGA
MslI 2924 CACGTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 96 CGCG CG^CG
NheI 2005 GCTAGC G^CTAGC
NsiI 858 ATGCAT ATGCA^T
PshAI 517 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3207 ATTAAT AT^TAAT
PstI 1554 CTGCAG CTGCA^G
PvuII 1970 CAGCTG CAG^CTG
RcaI 2833 TCATGA T^CATGA
ScaI 1462 AGTACT AGT^ACT
StyI 1662 CCAAGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AspI 904 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2837 GACTCAGTC
BbrPI 2061 CACGTG CAC^GTG
2924 CACGTG
BciVI 485 GTATCC GTATCC(6/5)
960 GTATCC
BclI 1746 TGATCA T^GATCA
2743 TGATCA
BglI 74 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
226 GCCTGAATGGC
BsaAI 2061 CACGTG [CT]AC^GT[GA]
2924 CACGTG
BsaI 123 GAGACC GGTCTC(1/5)
2652 GAGACC
Bst1107I 1603 GTATAC GTA^TAC
1688 GTATAC
BstXI 964 CCAGCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1969 CCAGCTGGATGG
BstYI 719 GGATCT [GA]^GATC[CT]
2213 GGATCT
BstZ17I 1603 GTATAC GTA^TAC
1688 GTATAC
DraI 1174 TTTAAA TTT^AAA
2870 TTTAAA
DraII 2327 GGGACCT [GA]G^G[ACGT]CC[CT]
2397 AGGACCT
EcoO109I 2327 GGGACCT [GA]G^G[ACGT]CC[CT]
2397 AGGACCT
FbaI 1746 TGATCA T^GATCA
2743 TGATCA
HaeII 447 AGCGCT [GA]GCGC^[CT]
1421 AGCGCC
HincII 2887 GTTAAC GT[CT]^[GA]AC
3129 GTTAAC
HindII 2887 GTTAAC GT[CT]^[GA]AC
3129 GTTAAC
HindIII 608 AAGCTT A^AGCTT
932 AAGCTT
HpaI 2887 GTTAAC GTT^AAC
3129 GTTAAC
MflI 719 GGATCT [GA]^GATC[CT]
2213 GGATCT
MspA1I 536 CAGCGG C[AC]G^C[GT]G
1970 CAGCTG
NdeI 861 CATATG CA^TATG
1750 CATATG
NspI 1125 ACATGC [GA]CATG^[CT]
2141 ACATGC
PflFI 904 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2837 GACTCAGTC
PmaCI 2061 CACGTG CAC^GTG
2924 CACGTG
PmlI 2061 CACGTG CAC^GTG
2924 CACGTG
PpuMI 2327 GGGACCT [GA]G^G[AT]CC[CT]
2397 AGGACCT
SacI 103 GAGCTC GAGCT^C
113 GAGCTC
SapI 730 GAAGAGC GCTCTTC(1/4)
1037 GAAGAGC
SpeI 1157 ACTAGT A^CTAGT
3170 ACTAGT
Tth111I 904 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2837 GACTCAGTC
XhoII 719 GGATCT [GA]^GATC[CT]
2213 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaII
BanII BbsI BbvCI BbvI BfaI
BmyI BpmI BpuAI BsaJI BsaWI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XmnI