##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AscI AspEI AviII AvrII
BbeI BciVI BfrI BglI BlnI
BseAI BsiEI BsiWI BspEI BssHII
Bst1107I BstBI BstXI BstZ17I CpoI
EagI Eam1105I EclXI Eco52I FseI
FspI HpaI KasI KspI MroI
NarI NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccII 9 CGCG CG^CG
AcyI 34 GGCGTC G[GA]^CG[CT]C
ApaI 721 GGGCCC GGGCC^C
AseI 5021 ATTAAT AT^TAAT
AsnI 5021 ATTAAT AT^TAAT
AvaI 419 CCCGAG C^[CT]CG[GA]G
BamHI 5218 GGATCC G^GATCC
BcgI 421 CGAGGAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3171 AGATCT A^GATCT
BsaBI 5846 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 34 GGCGTC G[GA]^CG[CT]C
BsoBI 419 CCCGAG C^[CT]CG[GA]G
BspDI 398 ATCGAT AT^CGAT
BsrBI 3197 GAGCGG CCGCTC(-3/-3)
BssSI 3869 CTCGTG CACGAG(-5/-1)
BstUI 9 CGCG CG^CG
ClaI 398 ATCGAT AT^CGAT
DrdI 3 GACCCACGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1768 GATATC GAT^ATC
Hin1I 34 GGCGTC G[GA]^CG[CT]C
HincII 209 GTTGAC GT[CT]^[GA]AC
HindII 209 GTTGAC GT[CT]^[GA]AC
MamI 5846 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3266 CAATTG C^AATTG
MluI 8 ACGCGT A^CGCGT
MunI 3266 CAATTG C^AATTG
MvnI 9 CGCG CG^CG
NdeI 4798 CATATG CA^TATG
PshBI 5021 ATTAAT AT^TAAT
ScaI 2904 AGTACT AGT^ACT
SspI 1167 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 513 GTCTAC GT^[AC][GT]AC
1220 GTAGAC
Alw44I 2474 GTGCAC G^TGCAC
3131 GTGCAC
Aor51HI 2484 AGCGCT AGC^GCT
4304 AGCGCT
ApaLI 2474 GTGCAC G^TGCAC
3131 GTGCAC
BalI 4380 TGGCCA TGG^CCA
4506 TGGCCA
BbrPI 2948 CACGTG CAC^GTG
3160 CACGTG
BbvCI 1472 GCTGAGG CCTCAGC(-5/-2)
5941 GCTGAGG
BclI 2344 TGATCA T^GATCA
3783 TGATCA
BlpI 2104 GCTGAGC GC^T[ACGT]AGC
4044 GCTTAGC
Bpu1102I 2104 GCTGAGC GC^T[ACGT]AGC
4044 GCTTAGC
BsaAI 2948 CACGTG [CT]AC^GT[GA]
3160 CACGTG
BsaWI 3111 ACCGGA [AT]^CCGG[AT]
3528 TCCGGT
BsmBI 36 CGTCTC CGTCTC(1/5)
1615 GAGACG
BspHI 1624 TCATGA T^CATGA
2826 TCATGA
BspLU11I 2639 ACATGT A^CATGT
4219 ACATGT
BsrFI 237 GCCGGC [GA]^CCGG[CT]
5406 GCCGGC
CelII 2104 GCTGAGC GC^T[ACGT]AGC
4044 GCTTAGC
Cfr10I 237 GCCGGC [GA]^CCGG[CT]
5406 GCCGGC
Eco47III 2484 AGCGCT AGC^GCT
4304 AGCGCT
EcoRI 781 GAATTC G^AATTC
1358 GAATTC
EcoT22I 402 ATGCAT ATGCA^T
2895 ATGCAT
FbaI 2344 TGATCA T^GATCA
3783 TGATCA
MluNI 4380 TGGCCA TGG^CCA
4506 TGGCCA
MscI 4380 TGGCCA TGG^CCA
4506 TGGCCA
NaeI 237 GCCGGC GCC^GGC
5406 GCCGGC
NgoMIV 237 GCCGGC G^CCGGC
5406 GCCGGC
NsiI 402 ATGCAT ATGCA^T
2895 ATGCAT
PmaCI 2948 CACGTG CAC^GTG
3160 CACGTG
PmlI 2948 CACGTG CAC^GTG
3160 CACGTG
RcaI 1624 TCATGA T^CATGA
2826 TCATGA
SmlI 648 CTCAAG C^T[CT][GA]AG
5531 CTTGAG
SphI 2679 GCATGC GCATG^C
2897 GCATGC
XbaI 949 TCTAGA T^CTAGA
1780 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I Asp718I AspHI AspI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspMI BsrDI BsrGI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HindIII
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmnI