##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BbeI
BbrPI BciVI BclI BfaI BfrI
BglII BlnI BsaAI BsaBI BseAI
BsiWI BspDI BspEI BspHI BspLU11I
BssHII Bst1107I BstAPI BstBI BstZ17I
ClaI DraI DraIII Eam1105I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI KpnI
MaeI MamI MfeI MluI MroI
MseI MunI NarI NheI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI SalI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspI SwaI Tru9I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1414 GTAGAC GT^[AC][GT]AC
Aor51HI 2630 AGCGCT AGC^GCT
AspI 2655 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1433 TGCGCA TGC^GCA
BalI 2168 TGGCCA TGG^CCA
BsaWI 976 TCCGGT [AT]^CCGG[AT]
BsmI 2438 GCATTC GAATGC(1/-1)
BsrDI 2291 GCAATG GCAATG(2/0)
BssSI 1081 CACGAG CACGAG(-5/-1)
BstEII 364 GGTGACC G^GT[ACGT]ACC
BstPI 364 GGTGACC G^GT[ACGT]ACC
DrdI 250 GACGTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2630 AGCGCT AGC^GCT
EcoO65I 364 GGTGACC G^GT[ACGT]ACC
FspI 1433 TGCGCA TGC^GCA
MluNI 2168 TGGCCA TGG^CCA
MscI 2168 TGGCCA TGG^CCA
MslI 2658 CATGTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 327 GCCGGC GCC^GGC
NcoI 2591 CCATGG C^CATGG
NdeI 157 CATATG CA^TATG
NgoMIV 327 GCCGGC G^CCGGC
NotI 2793 GCGGCCGC GC^GGCCGC
NruI 2490 TCGCGA TCG^CGA
PaeR7I 2496 CTCGAG C^TCGAG
PflFI 2655 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 1472 GCTCTTC GCTCTTC(1/4)
SbfI 915 CCTGCAGG CCTGCA^GG
SfiI 2169 GGCCAACGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 915 CCTGCAGG CCTGCA^GG
Tsp509I 2451 AATT ^AATT
Tth111I 2655 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2496 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 1497 GACGCC G[GA]^CG[CT]C
1626 GGCGTC
AocI 466 CCTGAGG CC^T[ACGT]AGG
1687 CCTGAGG
BamHI 118 GGATCC G^GATCC
1207 GGATCC
BbvCI 739 GCTGAGG CCTCAGC(-5/-2)
1603 GCTGAGG
BsaHI 1497 GACGCC G[GA]^CG[CT]C
1626 GGCGTC
BsgI 922 GTGCAG GTGCAG(16/14)
1462 GTGCAG
Bsp1407I 557 TGTACA T^GTACA
2339 TGTACA
BsrFI 185 ACCGGC [GA]^CCGG[CT]
327 GCCGGC
BsrGI 557 TGTACA T^GTACA
2339 TGTACA
BstXI 1478 CCATCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1715 CCAGCCTGCTGG
Bsu36I 466 CCTGAGG CC^T[ACGT]AGG
1687 CCTGAGG
Cfr10I 185 ACCGGC [GA]^CCGG[CT]
327 GCCGGC
CpoI 2575 CGGACCG CG^G[AT]CCG
2621 CGGACCG
EarI 1473 CTCTTC CTCTTC(1/4)
2199 GAAGAG
Eco81I 466 CCTGAGG CC^T[ACGT]AGG
1687 CCTGAGG
Hin1I 1497 GACGCC G[GA]^CG[CT]C
1626 GGCGTC
Ksp632I 1473 CTCTTC CTCTTC(1/4)
2199 GAAGAG
KspI 928 CCGCGG CCGC^GG
2103 CCGCGG
NspI 560 ACATGC [GA]CATG^[CT]
1151 ACATGC
PflMI 891 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
982 CCAGTCCCTGG
PvuII 721 CAGCTG CAG^CTG
1963 CAGCTG
RsrII 2575 CGGACCG CG^G[AT]CCG
2621 CGGACCG
SacII 928 CCGCGG CCGC^GG
2103 CCGCGG
SmlI 1131 CTTGAG C^T[CT][GA]AG
2496 CTCGAG
SspBI 557 TGTACA T^GTACA
2339 TGTACA
StuI 20 AGGCCT AGG^CCT
176 AGGCCT
Van91I 891 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
982 CCAGTCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcgI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XcmI
XhoII XmaCI XmaI