##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AccIII AclI AgeI Alw44I ApaLI
AscI AseI AsnI AspI AvrII
BbrPI BciVI BglII BlnI BsaAI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI EagI EclXI
Eco52I EcoO65I EcoRI EcoRV FseI
HpaI MfeI MluI MroI MunI
NdeI NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 1504 GACGTC GACGT^C
AccI 4169 GTCTAC GT^[AC][GT]AC
AflII 1612 CTTAAG C^TTAAG
AviII 1837 TGCGCA TGC^GCA
BamHI 2785 GGATCC G^GATCC
BbeI 2092 GGCGCC GGCGC^C
BbsI 5377 GAAGAC GAAGAC(2/6)
BfrI 1612 CTTAAG C^TTAAG
BlpI 3038 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3038 GCTCAGC GC^T[ACGT]AGC
BpuAI 5377 GAAGAC GAAGAC(2/6)
BsrFI 2558 GCCGGC [GA]^CCGG[CT]
CelII 3038 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2558 GCCGGC [GA]^CCGG[CT]
DraI 367 TTTAAA TTT^AAA
DrdI 3399 GACCCTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 4211 ATGCAT ATGCA^T
FspI 1837 TGCGCA TGC^GCA
HincII 1507 GTCAAC GT[CT]^[GA]AC
HindII 1507 GTCAAC GT[CT]^[GA]AC
HindIII 5725 AAGCTT A^AGCTT
KasI 2092 GGCGCC G^GCGCC
KspI 4 CCGCGG CCGC^GG
NaeI 2558 GCCGGC GCC^GGC
NarI 2092 GGCGCC GG^CGCC
NgoMIV 2558 GCCGGC G^CCGGC
NsiI 4211 ATGCAT ATGCA^T
PaeR7I 5263 CTCGAG C^TCGAG
SacI 1564 GAGCTC GAGCT^C
SacII 4 CCGCGG CCGC^GG
SexAI 3815 ACCAGGT A^CC[AT]GGT
SfoI 2092 GGCGCC GGC^GCC
SmaI 5201 CCCGGG CCC^GGG
SspI 490 AATATT AAT^ATT
XbaI 2525 TCTAGA T^CTAGA
XhoI 5263 CTCGAG C^TCGAG
XmaCI 5201 CCCGGG C^CCGGG
XmaI 5201 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 2448 GGTACC G^GTACC
3967 GGTACC
AccII 5 CGCG CG^CG
5506 CGCG
AflIII 413 ACATGT A^C[GA][CT]GT
1120 ACATGT
AhdI 2350 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4604 GACACACTGTC
Aor51HI 858 AGCGCT AGC^GCT
4353 AGCGCT
Asp718I 2448 GGTACC G^GTACC
3967 GGTACC
AspEI 2350 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4604 GACACACTGTC
BalI 650 TGGCCA TGG^CCA
1007 TGGCCA
BclI 947 TGATCA T^GATCA
3198 TGATCA
BspLU11I 413 ACATGT A^CATGT
1120 ACATGT
BsrBI 2019 CCGCTC CCGCTC(-3/-3)
1220 GAGCGG
BstUI 5 CGCG CG^CG
5506 CGCG
Eam1105I 2350 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4604 GACACACTGTC
Eco47III 858 AGCGCT AGC^GCT
4353 AGCGCT
EcoNI 2925 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5178 CCTCGGGAAGG
FbaI 947 TGATCA T^GATCA
3198 TGATCA
KpnI 2448 GGTACC GGTAC^C
3967 GGTACC
MaeII 1505 ACGT A^CGT
2780 ACGT
MluNI 650 TGGCCA TGG^CCA
1007 TGGCCA
MscI 650 TGGCCA TGG^CCA
1007 TGGCCA
MvnI 5 CGCG CG^CG
5506 CGCG
PvuII 758 CAGCTG CAG^CTG
5571 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BsaBI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI RcaI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI