##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AclI AgeI Alw44I Aor51HI
ApaLI AscI Asp718I AviII BbrPI
BcgI BcnI BlpI Bpu1102I BsaBI
BsiWI BspDI BsrFI BssHII BstBI
CelII Cfr10I ClaI CpoI Eco47III
FseI FspI HgaI KpnI KspI
MamI MfeI MluI MunI NaeI
NciI NgoMIV NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 13 TCCGGA T^CCGGA
AflII 5884 CTTAAG C^TTAAG
AhdI 3376 GACAAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3376 GACAAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1437 GAGCAC G[AT]GC[AT]^C
BamHI 1067 GGATCC G^GATCC
BfrI 5884 CTTAAG C^TTAAG
BsaAI 1307 CACGTA [CT]AC^GT[GA]
BsaWI 13 TCCGGA [AT]^CCGG[AT]
BseAI 13 TCCGGA T^CCGGA
BsiEI 229 CGGCCG CG[GA][CT]^CG
BsiHKAI 1437 GAGCAC G[AT]GC[AT]^C
BsmBI 3743 GAGACG CGTCTC(1/5)
Bsp1407I 5461 TGTACA T^GTACA
BspEI 13 TCCGGA T^CCGGA
BspHI 5387 TCATGA T^CATGA
BsrGI 5461 TGTACA T^GTACA
BssSI 3790 CTCGTG CACGAG(-5/-1)
Bst1107I 5303 GTATAC GTA^TAC
BstEII 3452 GGTCACC G^GT[ACGT]ACC
BstPI 3452 GGTCACC G^GT[ACGT]ACC
BstZ17I 5303 GTATAC GTA^TAC
DraIII 982 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 130 GACAGTCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 229 CGGCCG C^GGCCG
Eam1105I 3376 GACAAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 229 CGGCCG C^GGCCG
Eco52I 229 CGGCCG C^GGCCG
EcoO65I 3452 GGTCACC G^GT[ACGT]ACC
EcoRV 1105 GATATC GAT^ATC
MroI 13 TCCGGA T^CCGGA
NheI 1023 GCTAGC G^CTAGC
RcaI 5387 TCATGA T^CATGA
SmlI 5884 CTTAAG C^T[CT][GA]AG
SspBI 5461 TGTACA T^GTACA
StuI 2668 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AatII 810 GACGTC GACGT^C
1676 GACGTC
AccI 785 GTCTAC GT^[AC][GT]AC
5303 GTATAC
AccII 85 CGCG CG^CG
3635 CGCG
AvaI 294 CTCGGG C^[CT]CG[GA]G
706 CCCGAG
BalI 978 TGGCCA TGG^CCA
3037 TGGCCA
BbeI 88 GGCGCC GGCGC^C
3700 GGCGCC
BbsI 98 GAAGAC GAAGAC(2/6)
5756 GTCTTC
BbvCI 1553 CCTCAGC CCTCAGC(-5/-2)
2216 CCTCAGC
BciVI 2206 GGATAC GTATCC(6/5)
2737 GGATAC
BclI 482 TGATCA T^GATCA
3860 TGATCA
BglI 3432 GCCTTAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3765 GCCAGGACGGC
BpuAI 98 GAAGAC GAAGAC(2/6)
5756 GTCTTC
BsaI 2033 GAGACC GGTCTC(1/5)
1328 GGTCTC
BsoBI 294 CTCGGG C^[CT]CG[GA]G
706 CCCGAG
BsrBI 34 CCGCTC CCGCTC(-3/-3)
17 GAGCGG
BsrDI 963 GCAATG GCAATG(2/0)
3469 GCAATG
BstAPI 2240 GCAAGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3652 GCAACCTCTGC
BstUI 85 CGCG CG^CG
3635 CGCG
BstXI 3815 CCAAAGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4337 CCAGCTTGCTGG
EcoNI 407 CCTTCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1187 CCTGGAAAAGG
EcoRI 4193 GAATTC G^AATTC
5869 GAATTC
EcoT22I 398 ATGCAT ATGCA^T
3032 ATGCAT
FbaI 482 TGATCA T^GATCA
3860 TGATCA
HaeII 88 GGCGCC [GA]GCGC^[CT]
3700 GGCGCC
HpaI 2996 GTTAAC GTT^AAC
4136 GTTAAC
KasI 88 GGCGCC G^GCGCC
3700 GGCGCC
MluNI 978 TGGCCA TGG^CCA
3037 TGGCCA
MscI 978 TGGCCA TGG^CCA
3037 TGGCCA
MslI 441 CATGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1440 CACAAAAATG
MvnI 85 CGCG CG^CG
3635 CGCG
NarI 88 GGCGCC GG^CGCC
3700 GGCGCC
NcoI 1818 CCATGG C^CATGG
3985 CCATGG
NdeI 3556 CATATG CA^TATG
3910 CATATG
NsiI 398 ATGCAT ATGCA^T
3032 ATGCAT
PflMI 905 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2115 CCAGTGCATGG
PpuMI 2392 GGGTCCC [GA]G^G[AT]CC[CT]
5639 AGGACCT
ScaI 773 AGTACT AGT^ACT
3819 AGTACT
SfoI 88 GGCGCC GGC^GCC
3700 GGCGCC
Van91I 905 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2115 CCAGTGCATGG
XcmI 1809 CCACCTAATCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2111 CCAGCCAGTGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI AseI AsnI Asp700I AspI
AvaII AvrII BanI BanII BbvI
BfaI BglII BlnI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspLU11I BspMI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PflFI
PleI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XhoII XmnI