##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI AspI BbrPI BclI
BfrI BsaAI BsaBI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI Eam1105I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HpaI KpnI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SalI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 387 GTCTAC GT^[AC][GT]AC
AccIII 889 TCCGGA T^CCGGA
AgeI 1309 ACCGGT A^CCGGT
Aor51HI 1199 AGCGCT AGC^GCT
AvrII 2174 CCTAGG C^CTAGG
BalI 1403 TGGCCA TGG^CCA
BciVI 1846 GTATCC GTATCC(6/5)
BglII 1696 AGATCT A^GATCT
BlnI 2174 CCTAGG C^CTAGG
BseAI 889 TCCGGA T^CCGGA
BsiEI 182 CGGCCG CG[GA][CT]^CG
BsmBI 3072 CGTCTC CGTCTC(1/5)
BspEI 889 TCCGGA T^CCGGA
BsrDI 134 CATTGC GCAATG(2/0)
BstXI 3331 CCATGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1737 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 182 CGGCCG C^GGCCG
EclXI 182 CGGCCG C^GGCCG
Eco47III 1199 AGCGCT AGC^GCT
Eco52I 182 CGGCCG C^GGCCG
HincII 1233 GTCAAC GT[CT]^[GA]AC
HindII 1233 GTCAAC GT[CT]^[GA]AC
HindIII 2768 AAGCTT A^AGCTT
MluNI 1403 TGGCCA TGG^CCA
MroI 889 TCCGGA T^CCGGA
MscI 1403 TGGCCA TGG^CCA
MseI 3704 TTAA T^TAA
PaeR7I 3093 CTCGAG C^TCGAG
PflMI 3301 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1309 ACCGGT A^CCGGT
PshAI 2823 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 2105 CCTGCAGG CCTGCA^GG
ScaI 1879 AGTACT AGT^ACT
SfiI 2660 GGCCAGTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1870 GCATGC GCATG^C
Sse8387I 2105 CCTGCAGG CCTGCA^GG
TfiI 1428 GAATC G^A[AT]TC
Tru9I 3704 TTAA T^TAA
Van91I 3301 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 961 CCACACTCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3093 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 1094 GAATTT [GA]^AATT[CT]
3693 AAATTT
AflIII 1237 ACGTGT A^C[GA][CT]GT
1669 ACGTGT
AocI 3141 CCTCAGG CC^T[ACGT]AGG
3633 CCTGAGG
ApoI 1094 GAATTT [GA]^AATT[CT]
3693 AAATTT
Asp700I 293 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1694 GAAGATCTTC
AviII 130 TGCGCA TGC^GCA
1603 TGCGCA
BamHI 119 GGATCC G^GATCC
3558 GGATCC
BbsI 1262 GAAGAC GAAGAC(2/6)
2615 GAAGAC
BlpI 543 GCTGAGC GC^T[ACGT]AGC
2357 GCTCAGC
Bpu1102I 543 GCTGAGC GC^T[ACGT]AGC
2357 GCTCAGC
BpuAI 1262 GAAGAC GAAGAC(2/6)
2615 GAAGAC
Bsu36I 3141 CCTCAGG CC^T[ACGT]AGG
3633 CCTGAGG
CelII 543 GCTGAGC GC^T[ACGT]AGC
2357 GCTCAGC
DrdI 2785 GACAGAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3596 GACCTCCCGGTC
Eco81I 3141 CCTCAGG CC^T[ACGT]AGG
3633 CCTGAGG
FspI 130 TGCGCA TGC^GCA
1603 TGCGCA
NaeI 263 GCCGGC GCC^GGC
712 GCCGGC
NgoMIV 263 GCCGGC G^CCGGC
712 GCCGGC
SexAI 1173 ACCTGGT A^CC[AT]GGT
1492 ACCTGGT
SmaI 18 CCCGGG CCC^GGG
427 CCCGGG
StuI 3571 AGGCCT AGG^CCT
3630 AGGCCT
Tsp509I 1095 AATT ^AATT
3694 AATT
XmaCI 18 CCCGGG C^CCGGG
427 CCCGGG
XmaI 18 CCCGGG C^CCGGG
427 CCCGGG
XmnI 293 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1694 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbvCI
BbvI BcgI BcnI BfaI BglI
BmyI BpmI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssHII
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacI SacII SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StyI TaqI TseI
Tsp45I TspRI TthHB8I XhoII