##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI AspHI
AviII BbeI BciVI BfrI BglI
BlpI Bpu1102I BsaI BseAI BsiEI
BsiHKAI BsiWI BsmBI BspDI BspEI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FseI FspI KasI KspI
MfeI MluI MroI MspA1I MunI
MvnI NaeI NarI NgoMIV NotI
NruI NsiI PacI PinAI PmeI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SbfI SfiI SfoI
SgrAI SnaBI SpeI SphI Sse8387I
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 345 GGTACC G^GTACC
AcyI 184 GGCGTC G[GA]^CG[CT]C
Asp718I 345 GGTACC G^GTACC
AspI 201 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 5141 GGATCC G^GATCC
BanI 345 GGTACC G^G[CT][GA]CC
BbrPI 2333 CACGTG CAC^GTG
BbvCI 1294 CCTCAGC CCTCAGC(-5/-2)
BcgI 2297 GCAAAAAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 5217 TGATCA T^GATCA
BsaAI 2333 CACGTG [CT]AC^GT[GA]
BsaHI 184 GGCGTC G[GA]^CG[CT]C
BsaWI 3438 ACCGGA [AT]^CCGG[AT]
BseRI 1292 CTCCTC GAGGAG(10/8)
BsgI 109 CTGCAC GTGCAG(16/14)
BsmI 4024 GAATGC GAATGC(1/-1)
BspMI 1966 GCAGGT ACCTGC(4/8)
BstAPI 2332 GCACGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1332 TTCGAA TT^CGAA
EcoNI 4737 CCTTGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 5217 TGATCA T^GATCA
HaeII 2204 AGCGCC [GA]GCGC^[CT]
HgaI 185 GCGTC GACGC(5/10)
Hin1I 184 GGCGTC G[GA]^CG[CT]C
HpaI 2808 GTTAAC GTT^AAC
KpnI 345 GGTACC GGTAC^C
NcoI 4474 CCATGG C^CATGG
NdeI 2152 CATATG CA^TATG
NheI 2067 GCTAGC G^CTAGC
NspV 1332 TTCGAA TT^CGAA
PflFI 201 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3453 CCATAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2333 CACGTG CAC^GTG
PmlI 2333 CACGTG CAC^GTG
PshAI 231 GACGAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 168 GTCGAC G^TCGAC
SfuI 1332 TTCGAA TT^CGAA
SmaI 2567 CCCGGG CCC^GGG
StuI 3821 AGGCCT AGG^CCT
Tth111I 201 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3453 CCATAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2567 CCCGGG C^CCGGG
XmaI 2567 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 168 GTCGAC GT^[AC][GT]AC
5272 GTCTAC
AflIII 843 ACATGT A^C[GA][CT]GT
3640 ACATGT
AhdI 1229 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3105 GACAAAAAGTC
AlwNI 2915 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
4542 CAGAACCTG
Asp700I 1279 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2623 GAAATACTTC
AspEI 1229 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3105 GACAAAAAGTC
AvrII 1219 CCTAGG C^CTAGG
5145 CCTAGG
BalI 50 TGGCCA TGG^CCA
4712 TGGCCA
BcnI 2567 CCCGG CC^[GC]GG
2568 CCGGG
BglII 2503 AGATCT A^GATCT
3654 AGATCT
BlnI 1219 CCTAGG C^CTAGG
5145 CCTAGG
BsaBI 3943 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5212 GATGTTGATC
BspLU11I 843 ACATGT A^CATGT
3640 ACATGT
CfoI 2205 GCGC GCG^C
2295 GCGC
EaeI 50 TGGCCA [CT]^GGCC[GA]
4712 TGGCCA
Eam1105I 1229 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3105 GACAAAAAGTC
HapII 2568 CCGG C^CGG
3439 CCGG
HhaI 2205 GCGC GCG^C
2295 GCGC
HinP1I 2205 GCGC G^CGC
2295 GCGC
HindIII 4334 AAGCTT A^AGCTT
5063 AAGCTT
HpaII 2568 CCGG C^CGG
3439 CCGG
MaeII 427 ACGT A^CGT
2334 ACGT
MamI 3943 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5212 GATGTTGATC
MluNI 50 TGGCCA TGG^CCA
4712 TGGCCA
MscI 50 TGGCCA TGG^CCA
4712 TGGCCA
MspI 2568 CCGG C^CGG
3439 CCGG
NciI 2567 CCCGG CC^[GC]GG
2568 CCGGG
SapI 2407 GAAGAGC GCTCTTC(1/4)
2891 GAAGAGC
XcmI 2887 CCAAGAAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5244 CCAAGTATAACCTGG
XmnI 1279 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2623 GAAATACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaI AvaII BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspHI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PaeR7I PleI PpuMI PstI
RcaI RsaI Sau3AI Sau96I ScaI
ScrFI SexAI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoI XhoII