##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AclI AcsI AflII AgeI ApoI
AscI AseI AsnI Asp700I BfrI
BsaBI Bsp1407I BspDI BspHI BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
DraI EcoRI EcoRV EcoT22I FseI
HindIII HpaI MamI MfeI MluI
MunI NdeI NotI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI ScaI SfuI
SgrAI SnaBI SpeI SphI SspBI
SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 3389 GACGTC GACGT^C
Acc65I 748 GGTACC G^GTACC
AocI 4278 CCTGAGG CC^T[ACGT]AGG
Aor51HI 71 AGCGCT AGC^GCT
ApaI 3365 GGGCCC GGGCC^C
Asp718I 748 GGTACC G^GTACC
AspI 3686 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 37 CCTAGG C^CTAGG
BamHI 1448 GGATCC G^GATCC
BbrPI 4500 CACGTG CAC^GTG
BciVI 2170 GGATAC GTATCC(6/5)
BclI 5402 TGATCA T^GATCA
BlnI 37 CCTAGG C^CTAGG
BsiWI 4993 CGTACG C^GTACG
BspLU11I 3806 ACATGT A^CATGT
BssSI 3555 CACGAG CACGAG(-5/-1)
Bsu36I 4278 CCTGAGG CC^T[ACGT]AGG
CpoI 2816 CGGACCG CG^G[AT]CCG
DraIII 5395 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 71 AGCGCT AGC^GCT
Eco81I 4278 CCTGAGG CC^T[ACGT]AGG
FbaI 5402 TGATCA T^GATCA
HincII 2441 GTCGAC GT[CT]^[GA]AC
HindII 2441 GTCGAC GT[CT]^[GA]AC
KpnI 748 GGTACC GGTAC^C
NaeI 3280 GCCGGC GCC^GGC
NcoI 2393 CCATGG C^CATGG
NgoMIV 3280 GCCGGC G^CCGGC
NheI 4775 GCTAGC G^CTAGC
NruI 76 TCGCGA TCG^CGA
PflFI 3686 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4500 CACGTG CAC^GTG
PmlI 4500 CACGTG CAC^GTG
RsrII 2816 CGGACCG CG^G[AT]CCG
SalI 2441 GTCGAC G^TCGAC
SspI 2466 AATATT AAT^ATT
StuI 1329 AGGCCT AGG^CCT
Tth111I 3686 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 2441 GTCGAC GT^[AC][GT]AC
2613 GTCTAC
AhdI 46 GACTCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5247 GACACCTGGTC
Alw44I 2355 GTGCAC G^TGCAC
5259 GTGCAC
ApaLI 2355 GTGCAC G^TGCAC
5259 GTGCAC
AspEI 46 GACTCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5247 GACACCTGGTC
BcgI 2593 CGAAGCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3804 GCACATGTCTCG
BsaAI 4500 CACGTG [CT]AC^GT[GA]
4995 TACGTG
BsmBI 1562 CGTCTC CGTCTC(1/5)
1508 GAGACG
BsrDI 373 CATTGC GCAATG(2/0)
126 GCAATG
DrdI 525 GACTGCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3389 GACGTCCCGGTC
EagI 29 CGGCCG C^GGCCG
3829 CGGCCG
Eam1105I 46 GACTCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5247 GACACCTGGTC
EclXI 29 CGGCCG C^GGCCG
3829 CGGCCG
Eco52I 29 CGGCCG C^GGCCG
3829 CGGCCG
KspI 1597 CCGCGG CCGC^GG
1912 CCGCGG
MseI 583 TTAA T^TAA
624 TTAA
PaeR7I 922 CTCGAG C^TCGAG
3215 CTCGAG
SacII 1597 CCGCGG CCGC^GG
1912 CCGCGG
SapI 1467 GAAGAGC GCTCTTC(1/4)
3421 GCTCTTC
SbfI 3845 CCTGCAGG CCTGCA^GG
4346 CCTGCAGG
SfiI 3366 GGCCCCTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3830 GGCCGACAAGGCC
Sse8387I 3845 CCTGCAGG CCTGCA^GG
4346 CCTGCAGG
Tru9I 583 TTAA T^TAA
624 TTAA
XhoI 922 CTCGAG C^TCGAG
3215 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AspHI
AvaI AvaII AviII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspEI BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SfoI SmaI SmlI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI