##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AspI BbeI
BcgI BfrI BsaBI BsiWI BsmBI
Bsp1407I BspHI BsrGI BssHII BstBI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I FseI HpaI KasI
MamI MfeI MluI MunI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PshAI
PshBI Psp1406I RcaI RsrII SalI
SbfI SexAI SfoI SfuI SgrAI
SpeI Sse8387I SspBI SwaI Tth111I
XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 2498 GGTACC G^GTACC
AccIII 2897 TCCGGA T^CCGGA
Asp718I 2498 GGTACC G^GTACC
AviII 299 TGCGCA TGC^GCA
AvrII 4757 CCTAGG C^CTAGG
BbrPI 610 CACGTG CAC^GTG
BbvCI 5311 CCTCAGC CCTCAGC(-5/-2)
BlnI 4757 CCTAGG C^CTAGG
BlpI 5361 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 5361 GCTTAGC GC^T[ACGT]AGC
BsaI 2159 GAGACC GGTCTC(1/5)
BseAI 2897 TCCGGA T^CCGGA
BsmI 5799 GAATGC GAATGC(1/-1)
BspDI 2932 ATCGAT AT^CGAT
BspEI 2897 TCCGGA T^CCGGA
Bst1107I 3313 GTATAC GTA^TAC
BstZ17I 3313 GTATAC GTA^TAC
CelII 5361 GCTTAGC GC^T[ACGT]AGC
ClaI 2932 ATCGAT AT^CGAT
DrdI 3706 GACAAAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 299 TGCGCA TGC^GCA
KpnI 2498 GGTACC GGTAC^C
KspI 2605 CCGCGG CCGC^GG
MroI 2897 TCCGGA T^CCGGA
PaeR7I 778 CTCGAG C^TCGAG
PmaCI 610 CACGTG CAC^GTG
PmeI 3654 GTTTAAAC GTTT^AAAC
PmlI 610 CACGTG CAC^GTG
SacII 2605 CCGCGG CCGC^GG
SnaBI 5631 TACGTA TAC^GTA
SspI 4841 AATATT AAT^ATT
StuI 1286 AGGCCT AGG^CCT
XbaI 5470 TCTAGA T^CTAGA
XhoI 778 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccII 1037 CGCG CG^CG
2606 CGCG
AcyI 988 GACGCC G[GA]^CG[CT]C
1219 GACGCC
AocI 750 CCTCAGG CC^T[ACGT]AGG
1357 CCTGAGG
ApaI 111 GGGCCC GGGCC^C
2592 GGGCCC
BciVI 1446 GTATCC GTATCC(6/5)
5283 GTATCC
BclI 4911 TGATCA T^GATCA
5157 TGATCA
BsaAI 610 CACGTG [CT]AC^GT[GA]
5631 TACGTA
BsaHI 988 GACGCC G[GA]^CG[CT]C
1219 GACGCC
BsiEI 741 CGATCG CG[GA][CT]^CG
2934 CGATCG
BsrFI 803 ACCGGC [GA]^CCGG[CT]
1310 ACCGGC
BssSI 2651 CACGAG CACGAG(-5/-1)
1730 CTCGTG
BstEII 870 GGTCACC G^GT[ACGT]ACC
3990 GGTCACC
BstPI 870 GGTCACC G^GT[ACGT]ACC
3990 GGTCACC
BstUI 1037 CGCG CG^CG
2606 CGCG
Bsu36I 750 CCTCAGG CC^T[ACGT]AGG
1357 CCTGAGG
Cfr10I 803 ACCGGC [GA]^CCGG[CT]
1310 ACCGGC
Eco81I 750 CCTCAGG CC^T[ACGT]AGG
1357 CCTGAGG
EcoO65I 870 GGTCACC G^GT[ACGT]ACC
3990 GGTCACC
EcoRI 4589 GAATTC G^AATTC
4600 GAATTC
EcoRV 1102 GATATC GAT^ATC
5057 GATATC
EcoT22I 559 ATGCAT ATGCA^T
5801 ATGCAT
FbaI 4911 TGATCA T^GATCA
5157 TGATCA
HaeII 1229 AGCGCC [GA]GCGC^[CT]
1246 AGCGCC
Hin1I 988 GACGCC G[GA]^CG[CT]C
1219 GACGCC
HindIII 4335 AAGCTT A^AGCTT
5539 AAGCTT
MvnI 1037 CGCG CG^CG
2606 CGCG
NdeI 791 CATATG CA^TATG
5824 CATATG
NsiI 559 ATGCAT ATGCA^T
5801 ATGCAT
PvuI 741 CGATCG CGAT^CG
2934 CGATCG
SacI 103 GAGCTC GAGCT^C
5370 GAGCTC
SapI 2754 GAAGAGC GCTCTTC(1/4)
3939 GAAGAGC
ScaI 140 AGTACT AGT^ACT
4921 AGTACT
SfiI 834 GGCCAACTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2674 GGCCGTGGTGGCC
SmaI 245 CCCGGG CCC^GGG
1968 CCCGGG
SphI 917 GCATGC GCATG^C
1139 GCATGC
XmaCI 245 CCCGGG C^CCGGG
1968 CCCGGG
XmaI 245 CCCGGG C^CCGGG
1968 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrBI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII