##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AccI AccII AccIII AgeI AhdI
Aor51HI ApaI AscI AspEI AvaI
AviII BalI BanII BbeI BbrPI
BcgI BciVI BcnI BglI BsaAI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BsrBI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CfoI Cfr10I
ClaI CpoI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I FseI FspI HaeII HgaI
HhaI HinP1I HindIII KasI KspI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PvuI RsrII SacI
SacII SalI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SwaI Van91I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 3078 GACGTC GACGT^C
AciI 4673 CCGC CCGC(-3/-1)
AclI 2640 AACGTT AA^CGTT
AcyI 3078 GACGTC G[GA]^CG[CT]C
Alw44I 4340 GTGCAC G^TGCAC
ApaLI 4340 GTGCAC G^TGCAC
AspI 3205 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2487 CCTAGG C^CTAGG
BamHI 1041 GGATCC G^GATCC
BlnI 2487 CCTAGG C^CTAGG
BlpI 993 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 993 GCTCAGC GC^T[ACGT]AGC
BsaBI 3219 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3078 GACGTC G[GA]^CG[CT]C
BsgI 1801 GTGCAG GTGCAG(16/14)
Bsp1407I 3377 TGTACA T^GTACA
BsrGI 3377 TGTACA T^GTACA
BstXI 2499 CCAGAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 993 GCTCAGC GC^T[ACGT]AGC
DsaI 5029 CCATGG C^C[GA][CT]GG
EcoRV 1820 GATATC GAT^ATC
EcoT22I 3399 ATGCAT ATGCA^T
FauI 4672 CCCGC CCCGC(4/6)
HapII 5185 CCGG C^CGG
Hin1I 3078 GACGTC G[GA]^CG[CT]C
HincII 5108 GTTAAC GT[CT]^[GA]AC
HindII 5108 GTTAAC GT[CT]^[GA]AC
HpaI 5108 GTTAAC GTT^AAC
HpaII 5185 CCGG C^CGG
MamI 3219 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 31 CAGCTG C[AC]G^C[GT]G
MspI 5185 CCGG C^CGG
NcoI 5029 CCATGG C^CATGG
NdeI 1667 CATATG CA^TATG
NsiI 3399 ATGCAT ATGCA^T
PflFI 3205 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 223 GACATTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2640 AACGTT AA^CGTT
PvuII 31 CAGCTG CAG^CTG
SapI 2796 GAAGAGC GCTCTTC(1/4)
SbfI 1480 CCTGCAGG CCTGCA^GG
Sse8387I 1480 CCTGCAGG CCTGCA^GG
SspBI 3377 TGTACA T^GTACA
Tth111I 3205 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3275 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 1923 GGTACC G^GTACC
4590 GGTACC
AflII 3479 CTTAAG C^TTAAG
4889 CTTAAG
AflIII 415 ACATGT A^C[GA][CT]GT
4766 ACATGT
AlwNI 463 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
3864 CAGGATCTG
Asp700I 2544 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4627 GAATTATTTC
Asp718I 1923 GGTACC G^GTACC
4590 GGTACC
AspHI 2 GAGCAC G[AT]GC[AT]^C
4340 GTGCAC
BclI 11 TGATCA T^GATCA
4108 TGATCA
BfrI 3479 CTTAAG C^TTAAG
4889 CTTAAG
BglII 2253 AGATCT A^GATCT
2625 AGATCT
BmyI 2 GAGCAC G[AGT]GC[ACT]^C
4340 GTGCAC
BsaI 2278 GGTCTC GGTCTC(1/5)
2509 GGTCTC
BseRI 2231 CTCCTC GAGGAG(10/8)
2674 CTCCTC
BsiHKAI 2 GAGCAC G[AT]GC[AT]^C
4340 GTGCAC
BsmFI 2069 GGGAC GGGAC(10/14)
5427 GTCCC
BsmI 1539 GAATGC GAATGC(1/-1)
5409 GAATGC
Bsp1286I 2 GAGCAC G[AGT]GC[ACT]^C
4340 GTGCAC
BspLU11I 415 ACATGT A^CATGT
4766 ACATGT
BspMI 2907 ACCTGC ACCTGC(4/8)
3369 GCAGGT
DraII 629 AGGACCC [GA]G^G[ACGT]CC[CT]
2904 AGGACCT
EcoNI 1965 CCTGTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3303 CCTCAGGAAGG
EcoO109I 629 AGGACCC [GA]G^G[ACGT]CC[CT]
2904 AGGACCT
EcoRI 663 GAATTC G^AATTC
3692 GAATTC
FbaI 11 TGATCA T^GATCA
4108 TGATCA
KpnI 1923 GGTACC GGTAC^C
4590 GGTACC
MslI 4472 CATAAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
5024 CATCACCATG
PpuMI 629 AGGACCC [GA]G^G[AT]CC[CT]
2904 AGGACCT
ScaI 2172 AGTACT AGT^ACT
2373 AGTACT
StuI 1485 AGGCCT AGG^CCT
3758 AGGCCT
XcmI 676 CCACTGAATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2339 CCATGATTTTCTTGG
XmnI 2544 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4627 GAATTATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AcsI AfaI AluI AlwI AocI
ApoI AseI AsnI AvaII BanI
BbsI BbvCI BbvI BfaI BpmI
BpuAI BsaJI BsiYI BslI BsmAI
BspHI BsrDI BsrI BssKI BssSI
BstF5I BstNI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraI EarI
Eco81I EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PshBI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII