##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccI AccIII AclI AgeI Aor51HI
ApaI AscI AseI AsnI AspI
AviII AvrII BglI BlnI BseAI
BsiWI BspDI BspEI BspLU11I BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI Eco47III EcoRV EcoT22I FseI
FspI HpaI KspI MluI MroI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Tth111I Van91I XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 1703 GGTACC G^GTACC
AflII 3946 CTTAAG C^TTAAG
AflIII 3445 ACACGT A^C[GA][CT]GT
Alw44I 2123 GTGCAC G^TGCAC
AocI 4645 CCTAAGG CC^T[ACGT]AGG
ApaLI 2123 GTGCAC G^TGCAC
Asp718I 1703 GGTACC G^GTACC
BbrPI 462 CACGTG CAC^GTG
BbvCI 5329 GCTGAGG CCTCAGC(-5/-2)
BciVI 3646 GTATCC GTATCC(6/5)
BfrI 3946 CTTAAG C^TTAAG
BsaBI 2170 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1725 CGGCCG CG[GA][CT]^CG
Bsp1407I 1807 TGTACA T^GTACA
BsrBI 469 GAGCGG CCGCTC(-3/-3)
BsrGI 1807 TGTACA T^GTACA
BstEII 5334 GGTGACC G^GT[ACGT]ACC
BstPI 5334 GGTGACC G^GT[ACGT]ACC
Bsu36I 4645 CCTAAGG CC^T[ACGT]AGG
DraI 1519 TTTAAA TTT^AAA
DrdI 5594 GACATAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1725 CGGCCG C^GGCCG
EclXI 1725 CGGCCG C^GGCCG
Eco52I 1725 CGGCCG C^GGCCG
Eco81I 4645 CCTAAGG CC^T[ACGT]AGG
EcoO65I 5334 GGTGACC G^GT[ACGT]ACC
KpnI 1703 GGTACC GGTAC^C
MamI 2170 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 657 CAATTG C^AATTG
MunI 657 CAATTG C^AATTG
NaeI 3455 GCCGGC GCC^GGC
NdeI 1709 CATATG CA^TATG
NgoMIV 3455 GCCGGC G^CCGGC
NheI 3228 GCTAGC G^CTAGC
PmaCI 462 CACGTG CAC^GTG
PmlI 462 CACGTG CAC^GTG
SapI 1228 GAAGAGC GCTCTTC(1/4)
SexAI 2363 ACCTGGT A^CC[AT]GGT
SphI 2942 GCATGC GCATG^C
SspBI 1807 TGTACA T^GTACA
XbaI 4501 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 1277 GACGTC GACGT^C
5307 GACGTC
BamHI 3029 GGATCC G^GATCC
5417 GGATCC
BanII 3560 GAGCTC G[GA]GC[CT]^C
4807 GAGCTC
BbeI 4721 GGCGCC GGCGC^C
5700 GGCGCC
BcgI 4947 CGAAAGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5173 GCAGGCCTTTCG
BclI 2175 TGATCA T^GATCA
2714 TGATCA
BcnI 402 CCGGG CC^[GC]GG
5392 CCCGG
BglII 246 AGATCT A^GATCT
2990 AGATCT
BlpI 4603 GCTCAGC GC^T[ACGT]AGC
4804 GCTGAGC
Bpu1102I 4603 GCTCAGC GC^T[ACGT]AGC
4804 GCTGAGC
BsaAI 386 CACGTA [CT]AC^GT[GA]
462 CACGTG
BsaWI 368 ACCGGA [AT]^CCGG[AT]
2761 TCCGGT
BsmI 3794 GAATGC GAATGC(1/-1)
5547 GAATGC
BspHI 4614 TCATGA T^CATGA
5291 TCATGA
BsrDI 1564 GCAATG GCAATG(2/0)
4529 GCAATG
CelII 4603 GCTCAGC GC^T[ACGT]AGC
4804 GCTGAGC
DraIII 1096 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1633 CACAGGGTG
FbaI 2175 TGATCA T^GATCA
2714 TGATCA
HgaI 2597 GACGC GACGC(5/10)
2490 GCGTC
HincII 364 GTTGAC GT[CT]^[GA]AC
2752 GTCAAC
HindII 364 GTTGAC GT[CT]^[GA]AC
2752 GTCAAC
KasI 4721 GGCGCC G^GCGCC
5700 GGCGCC
NarI 4721 GGCGCC GG^CGCC
5700 GGCGCC
NciI 402 CCGGG CC^[GC]GG
5392 CCCGG
PaeR7I 2741 CTCGAG C^TCGAG
3545 CTCGAG
PpuMI 931 GGGTCCT [GA]G^G[AT]CC[CT]
2924 AGGTCCT
PstI 2640 CTGCAG CTGCA^G
3059 CTGCAG
RcaI 4614 TCATGA T^CATGA
5291 TCATGA
SacI 3560 GAGCTC GAGCT^C
4807 GAGCTC
SfoI 4721 GGCGCC GGC^GCC
5700 GGCGCC
SspI 1000 AATATT AAT^ATT
1361 AATATT
XhoI 2741 CTCGAG C^TCGAG
3545 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI AlwI AlwNI ApoI
Asp700I AspEI AspHI AvaI AvaII
BalI BanI BbsI BbvI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eam1105I EarI EcoNI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NcoI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI