##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccI AccII AccIII AcyI
AgeI ApaI AscI AviII AvrII
BamHI BbeI BbrPI BcgI BclI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I CelII ClaI CpoI EagI
EclXI Eco52I EcoRV FbaI FseI
FspI HgaI Hin1I HpaI KasI
KspI MamI MluI MroI MvnI
NarI NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PstI PvuI
RsrII SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SnaBI
Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AclI 1112 AACGTT AA^CGTT
AhdI 5248 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3239 CCTTAGG CC^T[ACGT]AGG
Aor51HI 31 AGCGCT AGC^GCT
AspEI 5248 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3817 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1254 GGTCC G^G[AT]CC
BciVI 5228 GGATAC GTATCC(6/5)
BsmBI 2145 CGTCTC CGTCTC(1/5)
BspLU11I 4494 ACATGT A^CATGT
BsrFI 8 GCCGGC [GA]^CCGG[CT]
BstAPI 1441 GCACTCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1798 GGTGACC G^GT[ACGT]ACC
BstPI 1798 GGTGACC G^GT[ACGT]ACC
Bsu36I 3239 CCTTAGG CC^T[ACGT]AGG
Cfr10I 8 GCCGGC [GA]^CCGG[CT]
DraII 3115 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 37 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4463 GACAAGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 5248 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 31 AGCGCT AGC^GCT
Eco81I 3239 CCTTAGG CC^T[ACGT]AGG
EcoO109I 3115 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1798 GGTGACC G^GT[ACGT]ACC
HaeII 31 AGCGCT [GA]GCGC^[CT]
HincII 4472 GTCAAC GT[CT]^[GA]AC
HindII 4472 GTCAAC GT[CT]^[GA]AC
MfeI 4143 CAATTG C^AATTG
MunI 4143 CAATTG C^AATTG
NaeI 8 GCCGGC GCC^GGC
NdeI 4399 CATATG CA^TATG
NgoMIV 8 GCCGGC G^CCGGC
PflFI 3817 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3892 CCAACTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1112 AACGTT AA^CGTT
PvuII 2437 CAGCTG CAG^CTG
ScaI 1596 AGTACT AGT^ACT
SexAI 2950 ACCAGGT A^CC[AT]GGT
SmaI 4687 CCCGGG CCC^GGG
SpeI 108 ACTAGT A^CTAGT
StuI 2492 AGGCCT AGG^CCT
Tth111I 3817 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3892 CCAACTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 4687 CCCGGG C^CCGGG
XmaI 4687 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 1092 GGTACC G^GTACC
4365 GGTACC
AflII 758 CTTAAG C^TTAAG
1769 CTTAAG
AflIII 2171 ACACGT A^C[GA][CT]GT
4494 ACATGT
Alw44I 1439 GTGCAC G^TGCAC
2191 GTGCAC
AlwNI 344 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
576 CAGTGTCTG
ApaLI 1439 GTGCAC G^TGCAC
2191 GTGCAC
AseI 2531 ATTAAT AT^TAAT
2544 ATTAAT
AsnI 2531 ATTAAT AT^TAAT
2544 ATTAAT
Asp718I 1092 GGTACC G^GTACC
4365 GGTACC
AvaI 4687 CCCGGG C^[CT]CG[GA]G
4719 CCCGAG
BfrI 758 CTTAAG C^TTAAG
1769 CTTAAG
BsoBI 4687 CCCGGG C^[CT]CG[GA]G
4719 CCCGAG
Bsp1407I 468 TGTACA T^GTACA
4342 TGTACA
BspHI 1261 TCATGA T^CATGA
4975 TCATGA
BspMI 1822 ACCTGC ACCTGC(4/8)
1965 ACCTGC
BsrDI 3367 CATTGC GCAATG(2/0)
816 GCAATG
BsrGI 468 TGTACA T^GTACA
4342 TGTACA
BstXI 1564 CCAGTGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3928 CCAAGTAGCTGG
BstYI 1833 AGATCC [GA]^GATC[CT]
2279 AGATCC
DsaI 1282 CCATGG C^C[GA][CT]GG
1338 CCATGG
EcoNI 2477 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3119 CCTTTTCCAGG
EcoRI 613 GAATTC G^AATTC
2571 GAATTC
EcoT22I 2858 ATGCAT ATGCA^T
5323 ATGCAT
HindIII 1663 AAGCTT A^AGCTT
3141 AAGCTT
KpnI 1092 GGTACC GGTAC^C
4365 GGTACC
MflI 1833 AGATCC [GA]^GATC[CT]
2279 AGATCC
MspA1I 1745 CAGCGG C[AC]G^C[GT]G
2437 CAGCTG
NcoI 1282 CCATGG C^CATGG
1338 CCATGG
NsiI 2858 ATGCAT ATGCA^T
5323 ATGCAT
PshBI 2531 ATTAAT AT^TAAT
2544 ATTAAT
RcaI 1261 TCATGA T^CATGA
4975 TCATGA
SacI 2311 GAGCTC GAGCT^C
4112 GAGCTC
SspBI 468 TGTACA T^GTACA
4342 TGTACA
XbaI 3315 TCTAGA T^CTAGA
4945 TCTAGA
XcmI 3062 CCATGTTCTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4440 CCACAGTCTCCTTGG
XhoII 1833 AGATCC [GA]^GATC[CT]
2279 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EaeI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmnI