##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AocI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BamHI BbrPI
BbsI BcgI BciVI BclI BfrI
BglII BlnI BpuAI BseAI BsgI
BsiWI BsmBI BsmI BspEI BspHI
BspLU11I BspMI BsrBI BssHII Bst1107I
BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I CpoI DraI DraIII EagI
EclXI Eco52I Eco81I EcoRI EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI KspI MfeI MflI MluI
MroI MseI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I RcaI RsrII
SacII SalI SapI SbfI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI Tru9I Tth111I XbaI XhoII
XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AflIII 253 ACGTGT A^C[GA][CT]GT
AhdI 1649 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1541 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 556 AGCGCT AGC^GCT
ApaI 798 GGGCCC GGGCC^C
AspEI 1649 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1581 TGGCCA TGG^CCA
BbeI 1609 GGCGCC GGCGC^C
BbvCI 1628 GCTGAGG CCTCAGC(-5/-2)
BsaAI 158 TACGTG [CT]AC^GT[GA]
BsaBI 68 GATATCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 540 GAGACC GGTCTC(1/5)
BsaWI 1464 TCCGGT [AT]^CCGG[AT]
BsiEI 73 CGATCG CG[GA][CT]^CG
Bsp1407I 561 TGTACA T^GTACA
BspDI 71 ATCGAT AT^CGAT
BsrDI 1450 CATTGC GCAATG(2/0)
BsrGI 561 TGTACA T^GTACA
BstEII 1666 GGTGACC G^GT[ACGT]ACC
BstPI 1666 GGTGACC G^GT[ACGT]ACC
ClaI 71 ATCGAT AT^CGAT
DrdI 1348 GACCACGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1649 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 556 AGCGCT AGC^GCT
EcoO65I 1666 GGTGACC G^GT[ACGT]ACC
EcoRV 68 GATATC GAT^ATC
HincII 479 GTTGAC GT[CT]^[GA]AC
HindII 479 GTTGAC GT[CT]^[GA]AC
KasI 1609 GGCGCC G^GCGCC
MamI 68 GATATCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1581 TGGCCA TGG^CCA
MscI 1581 TGGCCA TGG^CCA
MslI 1289 CACCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1112 CAGCTG C[AC]G^C[GT]G
NaeI 615 GCCGGC GCC^GGC
NarI 1609 GGCGCC GG^CGCC
NgoMIV 615 GCCGGC G^CCGGC
NspI 765 GCATGC [GA]CATG^[CT]
PflMI 1167 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 491 CTGCAG CTGCA^G
PvuI 73 CGATCG CGAT^CG
PvuII 1112 CAGCTG CAG^CTG
ScaI 717 AGTACT AGT^ACT
SexAI 316 ACCAGGT A^CC[AT]GGT
SfiI 790 GGCCTTGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1609 GGCGCC GGC^GCC
SmaI 213 CCCGGG CCC^GGG
SphI 765 GCATGC GCATG^C
SspBI 561 TGTACA T^GTACA
Tsp509I 872 AATT ^AATT
Van91I 1167 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 213 CCCGGG C^CCGGG
XmaI 213 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 702 CGCG CG^CG
1571 CGCG
Alw44I 268 GTGCAC G^TGCAC
1272 GTGCAC
ApaLI 268 GTGCAC G^TGCAC
1272 GTGCAC
BanI 209 GGTGCC G^G[CT][GA]CC
1609 GGCGCC
BfaI 62 CTAG C^TAG
83 CTAG
BglI 791 GCCTTGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1414 GCCAGAGAGGC
BseRI 383 GAGGAG GAGGAG(10/8)
1181 GAGGAG
BsrFI 615 GCCGGC [GA]^CCGG[CT]
1248 ACCGGC
BssSI 1193 CACGAG CACGAG(-5/-1)
1616 CACGAG
BstUI 702 CGCG CG^CG
1571 CGCG
Cfr10I 615 GCCGGC [GA]^CCGG[CT]
1248 ACCGGC
EarI 827 CTCTTC CTCTTC(1/4)
1457 CTCTTC
Ksp632I 827 CTCTTC CTCTTC(1/4)
1457 CTCTTC
MaeI 62 CTAG C^TAG
83 CTAG
MvnI 702 CGCG CG^CG
1571 CGCG
NcoI 1438 CCATGG C^CATGG
1488 CCATGG
PpuMI 753 AGGACCC [GA]G^G[AT]CC[CT]
1221 GGGACCC
SacI 136 GAGCTC GAGCT^C
943 GAGCTC
SfcI 307 CTACAG C^T[GA][CT]AG
491 CTGCAG
StuI 352 AGGCCT AGG^CCT
960 AGGCCT
TfiI 178 GAATC G^A[AT]TC
341 GAATC
XcmI 327 CCATGCGGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1167 CCATGAGCTGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI AvaI AvaII BanII BbvI
BcnI BlpI BmyI BpmI Bpu1102I
BsaHI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PaeR7I PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XhoI