##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AvrII       BbeI        
BbrPI       BcgI        BfrI        BlnI        BsaBI       
BsaWI       BseAI       BsiEI       BsiWI       BspDI       
BspEI       BssHII      BstAPI      BstBI       BstEII      
BstPI       ClaI        CpoI        DraIII      DrdI        
EagI        EclXI       Eco47III    Eco52I      EcoO65I     
EcoT22I     FseI        HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MroI        
MunI        NarI        NdeI        NotI        NruI        
NsiI        NspV        PacI        PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        SwaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AcyI         2500  GGCGTC               G[GA]^CG[CT]C
AhdI         2311  GACGAATTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         2764  CCTCAGG              CC^T[ACGT]AGG
AspEI        2311  GACGAATTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII          30  TGCGCA               TGC^GCA
BciVI        2334  GGATAC               GTATCC(6/5)
BglI         3904  GCCTACATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        2263  AGATCT               A^GATCT
BsaAI        4353  CACGTA               [CT]AC^GT[GA]
BsaHI        2500  GGCGTC               G[GA]^CG[CT]C
BsmBI        2502  CGTCTC               CGTCTC(1/5)
BspLU11I     3786  ACATGT               A^CATGT
BsrDI        1593  CATTGC               GCAATG(2/0)
BsrFI        1208  GCCGGC               [GA]^CCGG[CT]
BssSI        1168  CACGAG               CACGAG(-5/-1)
Bst1107I     5095  GTATAC               GTA^TAC
BstZ17I      5095  GTATAC               GTA^TAC
Bsu36I       2764  CCTCAGG              CC^T[ACGT]AGG
Cfr10I       1208  GCCGGC               [GA]^CCGG[CT]
DraI         5133  TTTAAA               TTT^AAA
Eam1105I     2311  GACGAATTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2764  CCTCAGG              CC^T[ACGT]AGG
EcoRI        1887  GAATTC               G^AATTC
FspI           30  TGCGCA               TGC^GCA
Hin1I        2500  GGCGTC               G[GA]^CG[CT]C
MluI         4369  ACGCGT               A^CGCGT
NaeI         1208  GCCGGC               GCC^GGC
NgoMIV       1208  GCCGGC               G^CCGGC
NheI         1204  GCTAGC               G^CTAGC
PaeR7I       3409  CTCGAG               C^TCGAG
PshAI        2299  GACTCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI         1323  CCTGCAGG             CCTGCA^GG
ScaI         2933  AGTACT               AGT^ACT
Sse8387I     1323  CCTGCAGG             CCTGCA^GG
XbaI         1050  TCTAGA               T^CTAGA
XhoI         3409  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccI         3625  GTAGAC               GT^[AC][GT]AC
             5095  GTATAC              
Asp700I       561  GAAGGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5189  GAAAATTTTC          
AspHI        1231  GAGCTC               G[AT]GC[AT]^C
             1555  GAGCTC              
AspI         1828  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3036  GACTTTGTC           
BamHI          61  GGATCC               G^GATCC
             3769  GGATCC              
BsiHKAI      1231  GAGCTC               G[AT]GC[AT]^C
             1555  GAGCTC              
Bsp1407I     4776  TGTACA               T^GTACA
             4870  TGTACA              
BsrGI        4776  TGTACA               T^GTACA
             4870  TGTACA              
BstXI         836  CCACCCTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2339  CCACAGTCCTGG        
EcoNI         182  CCTTCTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4445  CCTTGGAGAGG         
EcoRV         492  GATATC               GAT^ATC
              799  GATATC              
HaeII        2200  AGCGCC               [GA]GCGC^[CT]
             2832  GGCGCT              
HgaI         2501  GCGTC                GACGC(5/10)
             3524  GCGTC               
HincII       1969  GTCAAC               GT[CT]^[GA]AC
             4983  GTTGAC              
HindII       1969  GTCAAC               GT[CT]^[GA]AC
             4983  GTTGAC              
PflFI        1828  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3036  GACTTTGTC           
SacI         1231  GAGCTC               GAGCT^C
             1555  GAGCTC              
SspBI        4776  TGTACA               T^GTACA
             4870  TGTACA              
SspI         4239  AATATT               AAT^ATT
             4975  AATATT              
StuI         1513  AGGCCT               AGG^CCT
             1954  AGGCCT              
Tth111I      1828  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3036  GACTTTGTC           
XmnI          561  GAAGGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5189  GAAAATTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  122 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApaI        ApoI        
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvCI       BbvI        BclI        BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaI        BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspHI       BspMI       BsrBI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
FbaI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI