##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

AatII       AccIII      AclI        AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AspEI       AspI        AviII       AvrII       BbrPI       
BcgI        BclI        BglI        BlnI        BlpI        
Bpu1102I    BseAI       BsiWI       BsmBI       BspEI       
BsrFI       BssHII      BstBI       BstEII      BstPI       
BstXI       Bsu36I      CelII       Cfr10I      CpoI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRV       FbaI        FseI        FspI        HpaI        
KspI        MluI        MroI        NaeI        NcoI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PvuI        RsrII       SacI        SacII       SbfI        
SfiI        SfuI        SgrAI       SnaBI       Sse8387I    
SwaI        Tth111I     XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

Acc65I        961  GGTACC               G^GTACC
AccII          92  CGCG                 CG^CG
AcyI          102  GGCGCC               G[GA]^CG[CT]C
AflII        2526  CTTAAG               C^TTAAG
AflIII       3901  ACATGT               A^C[GA][CT]GT
Asp718I       961  GGTACC               G^GTACC
AvaI         1035  CCCGGG               C^[CT]CG[GA]G
BamHI         174  GGATCC               G^GATCC
BbeI          102  GGCGCC               GGCGC^C
BbvCI        3594  CCTCAGC              CCTCAGC(-5/-2)
BfrI         2526  CTTAAG               C^TTAAG
BglII        1654  AGATCT               A^GATCT
BsaAI        4660  TACGTG               [CT]AC^GT[GA]
BsaHI         102  GGCGCC               G[GA]^CG[CT]C
BsaI         1404  GAGACC               GGTCTC(1/5)
BsaWI        2006  ACCGGA               [AT]^CCGG[AT]
BsgI          588  CTGCAC               GTGCAG(16/14)
BsiEI         295  CGACCG               CG[GA][CT]^CG
BsmI         3499  GCATTC               GAATGC(1/-1)
BsoBI        1035  CCCGGG               C^[CT]CG[GA]G
BspHI        5581  TCATGA               T^CATGA
BspLU11I     3901  ACATGT               A^CATGT
BssSI         167  CACGAG               CACGAG(-5/-1)
Bst1107I     3243  GTATAC               GTA^TAC
BstAPI       3871  GCACCTGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          92  CGCG                 CG^CG
BstZ17I      3243  GTATAC               GTA^TAC
DraII         968  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoNI        3812  CCTTTAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      968  GGGGCCC              [GA]G^G[ACGT]CC[CT]
HaeII         102  GGCGCC               [GA]GCGC^[CT]
HgaI           90  GACGC                GACGC(5/10)
Hin1I         102  GGCGCC               G[GA]^CG[CT]C
HincII        474  GTCGAC               GT[CT]^[GA]AC
HindII        474  GTCGAC               GT[CT]^[GA]AC
KasI          102  GGCGCC               G^GCGCC
KpnI          961  GGTACC               GGTAC^C
MfeI         3042  CAATTG               C^AATTG
MunI         3042  CAATTG               C^AATTG
MvnI           92  CGCG                 CG^CG
NarI          102  GGCGCC               GG^CGCC
NdeI         3149  CATATG               CA^TATG
RcaI         5581  TCATGA               T^CATGA
SalI          474  GTCGAC               G^TCGAC
SexAI        2037  ACCTGGT              A^CC[AT]GGT
SfoI          102  GGCGCC               GGC^GCC
SmaI         1035  CCCGGG               CCC^GGG
SphI         2217  GCATGC               GCATG^C
XmaCI        1035  CCCGGG               C^CCGGG
XmaI         1035  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

ApaI          215  GGGCCC               GGGCC^C
              969  GGGCCC              
AvaII         559  GGACC                G^G[AT]CC
             2631  GGTCC               
BalI         3286  TGGCCA               TGG^CCA
             4343  TGGCCA              
BanII         215  GGGCCC               G[GA]GC[CT]^C
              969  GGGCCC              
BcnI         1035  CCCGG                CC^[GC]GG
             1036  CCGGG               
BsaBI        1655  GATCTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1789  GATTATTATC          
BseRI         372  GAGGAG               GAGGAG(10/8)
             5641  GAGGAG              
Bsp1407I      543  TGTACA               T^GTACA
             1506  TGTACA              
BspDI         645  ATCGAT               AT^CGAT
             1002  ATCGAT              
BsrGI         543  TGTACA               T^GTACA
             1506  TGTACA              
BstYI         174  GGATCC               [GA]^GATC[CT]
             1654  AGATCT              
ClaI          645  ATCGAT               AT^CGAT
             1002  ATCGAT              
EaeI         3286  TGGCCA               [CT]^GGCC[GA]
             4343  TGGCCA              
EcoT14I      2375  CCTTGG               C^C[AT][AT]GG
             3195  CCTTGG              
HapII        1036  CCGG                 C^CGG
             2007  CCGG                
HpaII        1036  CCGG                 C^CGG
             2007  CCGG                
MamI         1655  GATCTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1789  GATTATTATC          
MflI          174  GGATCC               [GA]^GATC[CT]
             1654  AGATCT              
MluNI        3286  TGGCCA               TGG^CCA
             4343  TGGCCA              
MscI         3286  TGGCCA               TGG^CCA
             4343  TGGCCA              
MspI         1036  CCGG                 C^CGG
             2007  CCGG                
NciI         1035  CCCGG                CC^[GC]GG
             1036  CCGGG               
PvuII        1929  CAGCTG               CAG^CTG
             1982  CAGCTG              
SapI          458  GAAGAGC              GCTCTTC(1/4)
             2943  GCTCTTC             
ScaI          893  AGTACT               AGT^ACT
             1827  AGTACT              
SspBI         543  TGTACA               T^GTACA
             1506  TGTACA              
StuI         3192  AGGCCT               AGG^CCT
             3348  AGGCCT              
StyI         2375  CCTTGG               C^C[AT][AT]GG
             3195  CCTTGG              
Tsp45I        401  GTGAC                ^GT[GC]AC
             5094  GTGAC               
XbaI          610  TCTAGA               T^CTAGA
             4225  TCTAGA              
XhoII         174  GGATCC               [GA]^GATC[CT]
             1654  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AseI        AsnI        
Asp700I     AspHI       BanI        BbsI        BbvI        
BciVI       BfaI        BmyI        BpmI        BpuAI       
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrBI       BsrDI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
EarI        EcoRI       EcoRII      EcoT22I     FauI        
Fnu4HI      FokI        HaeIII      HhaI        HinP1I      
HindIII     HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspA1I      MvaI        
MwoI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PflMI       PleI        PshBI       PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SpeI        SspI        TaqI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     Van91I      XmnI