##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AccIII AflII AflIII
AgeI ApaI AscI Asp718I AviII
AvrII BcgI BciVI BfrI BlnI
BsaBI BsaWI BseAI BsiWI BsmI
BspDI BspEI BspLU11I BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DrdI EcoO65I EcoRI
FspI KpnI KspI MamI MluI
MroI NcoI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PshAI PvuI RsrII SacII SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 2460 GTCTAC GT^[AC][GT]AC
AclI 2437 AACGTT AA^CGTT
AhdI 512 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1542 AGCGCT AGC^GCT
AseI 4086 ATTAAT AT^TAAT
AsnI 4086 ATTAAT AT^TAAT
AspEI 512 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 94 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1594 GGATCC G^GATCC
BbeI 119 GGCGCC GGCGC^C
BbrPI 4034 CACGTG CAC^GTG
BglII 3255 AGATCT A^GATCT
BspHI 3940 TCATGA T^CATGA
BspMI 516 GCAGGT ACCTGC(4/8)
BsrDI 581 GCAATG GCAATG(2/0)
DraIII 4034 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 240 CCACGG C^C[GA][CT]GG
Eam1105I 512 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1542 AGCGCT AGC^GCT
EcoRV 3051 GATATC GAT^ATC
FseI 76 GGCCGGCC GGCCGG^CC
HpaI 2923 GTTAAC GTT^AAC
KasI 119 GGCGCC G^GCGCC
NarI 119 GGCGCC GG^CGCC
PflFI 94 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2928 CCAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4034 CACGTG CAC^GTG
PmeI 144 GTTTAAAC GTTT^AAAC
PmlI 4034 CACGTG CAC^GTG
PshBI 4086 ATTAAT AT^TAAT
Psp1406I 2437 AACGTT AA^CGTT
RcaI 3940 TCATGA T^CATGA
SacI 4468 GAGCTC GAGCT^C
SexAI 1298 ACCAGGT A^CC[AT]GGT
SfoI 119 GGCGCC GGC^GCC
SmaI 3283 CCCGGG CCC^GGG
SphI 4287 GCATGC GCATG^C
SspI 688 AATATT AAT^ATT
Tth111I 94 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2928 CCAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 4054 CCAGCTACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3283 CCCGGG C^CCGGG
XmaI 3283 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 119 GGCGCC G[GA]^CG[CT]C
1716 GACGCC
Alw44I 3338 GTGCAC G^TGCAC
3606 GTGCAC
AocI 1582 CCTCAGG CC^T[ACGT]AGG
3423 CCTCAGG
ApaLI 3338 GTGCAC G^TGCAC
3606 GTGCAC
Asp700I 390 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4387 GAAAGTCTTC
BclI 3689 TGATCA T^GATCA
4245 TGATCA
BglI 77 GCCGGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1399 GCCAGTGAGGC
BlpI 2573 GCTCAGC GC^T[ACGT]AGC
4208 GCTGAGC
Bpu1102I 2573 GCTCAGC GC^T[ACGT]AGC
4208 GCTGAGC
BsaAI 2463 TACGTG [CT]AC^GT[GA]
4034 CACGTG
BsaHI 119 GGCGCC G[GA]^CG[CT]C
1716 GACGCC
BsmBI 136 CGTCTC CGTCTC(1/5)
3640 GAGACG
BsrBI 1745 CCGCTC CCGCTC(-3/-3)
4211 GAGCGG
BstAPI 1959 GCAGCTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3892 GCAAGATTTGC
Bsu36I 1582 CCTCAGG CC^T[ACGT]AGG
3423 CCTCAGG
CelII 2573 GCTCAGC GC^T[ACGT]AGC
4208 GCTGAGC
Eco81I 1582 CCTCAGG CC^T[ACGT]AGG
3423 CCTCAGG
EcoT22I 2881 ATGCAT ATGCA^T
4285 ATGCAT
FbaI 3689 TGATCA T^GATCA
4245 TGATCA
HgaI 1716 GACGC GACGC(5/10)
68 GCGTC
Hin1I 119 GGCGCC G[GA]^CG[CT]C
1716 GACGCC
MfeI 1472 CAATTG C^AATTG
1916 CAATTG
MunI 1472 CAATTG C^AATTG
1916 CAATTG
NsiI 2881 ATGCAT ATGCA^T
4285 ATGCAT
XbaI 340 TCTAGA T^CTAGA
3065 TCTAGA
XmnI 390 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4387 GAAAGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII EaeI EagI EarI
EclXI Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII