##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccII AflII AgeI
Alw44I Aor51HI ApaLI AscI Asp718I
AvrII BbeI BbrPI BcgI BfrI
BlnI BsaI BsiEI BsiWI BsmBI
BspDI BspLU11I BsrFI BssHII BstEII
BstPI BstUI Cfr10I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I FseI KasI KpnI KspI
MluI MvnI NaeI NarI NgoMIV
NheI NotI NruI PacI PinAI
PmaCI PmeI PmlI PpuMI PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 1518 TCCGGA T^CCGGA
AclI 211 AACGTT AA^CGTT
AcyI 2976 GACGCC G[GA]^CG[CT]C
AflIII 1759 ACACGT A^C[GA][CT]GT
AhdI 1228 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4687 CCTCAGG CC^T[ACGT]AGG
ApaI 1589 GGGCCC GGGCC^C
AspEI 1228 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2292 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1043 TGCGCA TGC^GCA
BciVI 5273 GTATCC GTATCC(6/5)
BclI 1086 TGATCA T^GATCA
BglI 4407 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1760 CACGTA [CT]AC^GT[GA]
BsaHI 2976 GACGCC G[GA]^CG[CT]C
BseAI 1518 TCCGGA T^CCGGA
BsmI 1077 GAATGC GAATGC(1/-1)
BspEI 1518 TCCGGA T^CCGGA
BspMI 3052 GCAGGT ACCTGC(4/8)
BsrDI 3365 GCAATG GCAATG(2/0)
BssSI 2504 CTCGTG CACGAG(-5/-1)
Bst1107I 5433 GTATAC GTA^TAC
BstAPI 4729 GCAACGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 120 TTCGAA TT^CGAA
BstZ17I 5433 GTATAC GTA^TAC
Bsu36I 4687 CCTCAGG CC^T[ACGT]AGG
DraIII 3181 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1228 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4687 CCTCAGG CC^T[ACGT]AGG
EcoNI 3564 CCTACTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 625 GATATC GAT^ATC
FbaI 1086 TGATCA T^GATCA
FspI 1043 TGCGCA TGC^GCA
Hin1I 2976 GACGCC G[GA]^CG[CT]C
HpaI 2556 GTTAAC GTT^AAC
MfeI 5476 CAATTG C^AATTG
MroI 1518 TCCGGA T^CCGGA
MunI 5476 CAATTG C^AATTG
NspV 120 TTCGAA TT^CGAA
PflFI 2292 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4459 GACTTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 211 AACGTT AA^CGTT
ScaI 3653 AGTACT AGT^ACT
SfuI 120 TTCGAA TT^CGAA
StuI 4021 AGGCCT AGG^CCT
Tth111I 2292 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 4565 CCACCACAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwNI 4690 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
5452 CAGAAACTG
AseI 5216 ATTAAT AT^TAAT
5409 ATTAAT
AsnI 5216 ATTAAT AT^TAAT
5409 ATTAAT
BalI 943 TGGCCA TGG^CCA
4577 TGGCCA
BamHI 1353 GGATCC G^GATCC
4292 GGATCC
BanI 551 GGCACC G^G[CT][GA]CC
925 GGCACC
BlpI 165 GCTCAGC GC^T[ACGT]AGC
170 GCTAAGC
Bpu1102I 165 GCTCAGC GC^T[ACGT]AGC
170 GCTAAGC
BsaBI 4747 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5070 GATAATGATC
BsaWI 1518 TCCGGA [AT]^CCGG[AT]
2534 ACCGGA
BsgI 2705 CTGCAC GTGCAG(16/14)
4992 GTGCAG
Bsp1407I 31 TGTACA T^GTACA
951 TGTACA
BsrBI 3523 GAGCGG CCGCTC(-3/-3)
3985 GAGCGG
BsrGI 31 TGTACA T^GTACA
951 TGTACA
CelII 165 GCTCAGC GC^T[ACGT]AGC
170 GCTAAGC
EcoT22I 67 ATGCAT ATGCA^T
5503 ATGCAT
FauI 1289 CCCGC CCCGC(4/6)
3987 GCGGG
HaeII 3200 GGCGCT [GA]GCGC^[CT]
3968 AGCGCC
HincII 2556 GTTAAC GT[CT]^[GA]AC
4315 GTCAAC
HindII 2556 GTTAAC GT[CT]^[GA]AC
4315 GTCAAC
MamI 4747 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5070 GATAATGATC
MluNI 943 TGGCCA TGG^CCA
4577 TGGCCA
MscI 943 TGGCCA TGG^CCA
4577 TGGCCA
NsiI 67 ATGCAT ATGCA^T
5503 ATGCAT
PaeR7I 597 CTCGAG C^TCGAG
1336 CTCGAG
PflMI 1100 CCAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4391 CCAATTCTTGG
PshBI 5216 ATTAAT AT^TAAT
5409 ATTAAT
SacI 163 GAGCTC GAGCT^C
5346 GAGCTC
SapI 3901 GAAGAGC GCTCTTC(1/4)
4681 GCTCTTC
SspBI 31 TGTACA T^GTACA
951 TGTACA
Van91I 1100 CCAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4391 CCAATTCTTGG
XhoI 597 CTCGAG C^TCGAG
1336 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaI
AvaII BanII BbsI BbvCI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI