################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## AccIII AclI AgeI AhdI Aor51HI AscI AspEI AviII AvrII BbeI BglI BlnI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BssHII Bst1107I BstAPI BstBI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoNI FseI FspI HaeII KasI KspI MluI MroI NarI NheI NotI NruI NspV PacI PinAI PmeI Psp1406I PvuI RsrII SacII ScaI SfiI SfoI SfuI SgrAI SphI SwaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AatII 5959 GACGTC GACGT^C Acc65I 3151 GGTACC G^GTACC AflII 2008 CTTAAG C^TTAAG AflIII 722 ACACGT A^C[GA][CT]GT Alw44I 265 GTGCAC G^TGCAC ApaLI 265 GTGCAC G^TGCAC AseI 3342 ATTAAT AT^TAAT AsnI 3342 ATTAAT AT^TAAT Asp700I 3407 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 3151 GGTACC G^GTACC BcgI 588 CGACTCAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 2008 CTTAAG C^TTAAG BspHI 4602 TCATGA T^CATGA BsrDI 3538 GCAATG GCAATG(2/0) BstEII 1988 GGTGACC G^GT[ACGT]ACC BstPI 1988 GGTGACC G^GT[ACGT]ACC DraIII 2468 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 1988 GGTGACC G^GT[ACGT]ACC EcoRV 3249 GATATC GAT^ATC HpaI 4932 GTTAAC GTT^AAC KpnI 3151 GGTACC GGTAC^C NdeI 6003 CATATG CA^TATG PaeR7I 5738 CTCGAG C^TCGAG PshBI 3342 ATTAAT AT^TAAT RcaI 4602 TCATGA T^CATGA SalI 6033 GTCGAC G^TCGAC SbfI 4866 CCTGCAGG CCTGCA^GG SexAI 4381 ACCTGGT A^CC[AT]GGT SnaBI 1120 TACGTA TAC^GTA SpeI 4456 ACTAGT A^CTAGT Sse8387I 4866 CCTGCAGG CCTGCA^GG SspI 2941 AATATT AAT^ATT XhoI 5738 CTCGAG C^TCGAG XmnI 3407 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccI 5962 GTCTAC GT^[AC][GT]AC 6033 GTCGAC AccII 982 CGCG CG^CG 4188 CGCG BbrPI 723 CACGTG CAC^GTG 3494 CACGTG BciVI 1875 GTATCC GTATCC(6/5) 3935 GTATCC BclI 570 TGATCA T^GATCA 3530 TGATCA BglII 3409 AGATCT A^GATCT 5710 AGATCT BsaBI 2361 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5577 GATCGTGATC BsaWI 525 ACCGGA [AT]^CCGG[AT] 4062 ACCGGA BsmBI 4811 CGTCTC CGTCTC(1/5) 787 GAGACG BsmI 5169 GAATGC GAATGC(1/-1) 6079 GCATTC Bsp1407I 3842 TGTACA T^GTACA 6065 TGTACA BsrBI 89 CCGCTC CCGCTC(-3/-3) 3760 CCGCTC BsrFI 26 GCCGGC [GA]^CCGG[CT] 274 GCCGGC BsrGI 3842 TGTACA T^GTACA 6065 TGTACA BssSI 262 CTCGTG CACGAG(-5/-1) 4090 CTCGTG BstUI 982 CGCG CG^CG 4188 CGCG Cfr10I 26 GCCGGC [GA]^CCGG[CT] 274 GCCGGC DrdI 2 GACTGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5882 GACTGAGAAGTC EcoT22I 2158 ATGCAT ATGCA^T 2557 ATGCAT FbaI 570 TGATCA T^GATCA 3530 TGATCA HincII 4932 GTTAAC GT[CT]^[GA]AC 6033 GTCGAC HindII 4932 GTTAAC GT[CT]^[GA]AC 6033 GTCGAC HindIII 5763 AAGCTT A^AGCTT 6106 AAGCTT MamI 2361 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5577 GATCGTGATC MvnI 982 CGCG CG^CG 4188 CGCG NaeI 26 GCCGGC GCC^GGC 274 GCCGGC NcoI 1951 CCATGG C^CATGG 4594 CCATGG NgoMIV 26 GCCGGC G^CCGGC 274 GCCGGC NsiI 2158 ATGCAT ATGCA^T 2557 ATGCAT NspI 2247 ACATGC [GA]CATG^[CT] 6062 GCATGT PmaCI 723 CACGTG CAC^GTG 3494 CACGTG PmlI 723 CACGTG CAC^GTG 3494 CACGTG PshAI 810 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2955 GACAAGGGTC SapI 83 GAAGAGC GCTCTTC(1/4) 6013 GAAGAGC SmaI 608 CCCGGG CCC^GGG 1511 CCCGGG SspBI 3842 TGTACA T^GTACA 6065 TGTACA XbaI 3670 TCTAGA T^CTAGA 5895 TCTAGA XmaCI 608 CCCGGG C^CCGGG 1511 CCCGGG XmaI 608 CCCGGG C^CCGGG 1511 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 128 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AspI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MwoI NciI NdeII NlaIII NlaIV PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII