##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AvaI AvrII BbrPI BciVI BglI
BlnI BlpI Bpu1102I BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspEI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII Cfr10I CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
FseI HindIII KpnI KspI MfeI
MluI MroI MunI MvnI NaeI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI StuI SwaI Tth111I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 5565 GGCGCC G[GA]^CG[CT]C
AspHI 3294 GAGCTC G[AT]GC[AT]^C
AvaII 3594 GGACC G^G[AT]CC
AviII 4289 TGCGCA TGC^GCA
BamHI 4061 GGATCC G^GATCC
BanI 5565 GGCGCC G^G[CT][GA]CC
BbeI 5565 GGCGCC GGCGC^C
BcgI 5599 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 5415 CCGGG CC^[GC]GG
BsaAI 1803 TACGTG [CT]AC^GT[GA]
BsaBI 2979 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 5565 GGCGCC G[GA]^CG[CT]C
BsiHKAI 3294 GAGCTC G[AT]GC[AT]^C
BspDI 4456 ATCGAT AT^CGAT
BstAPI 5625 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 4456 ATCGAT AT^CGAT
DraII 3593 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 3571 CCATGG C^C[GA][CT]GG
EcoO109I 3593 AGGACCC [GA]G^G[ACGT]CC[CT]
FspI 4289 TGCGCA TGC^GCA
HaeII 5565 GGCGCC [GA]GCGC^[CT]
HapII 5415 CCGG C^CGG
Hin1I 5565 GGCGCC G[GA]^CG[CT]C
HincII 3324 GTTAAC GT[CT]^[GA]AC
HindII 3324 GTTAAC GT[CT]^[GA]AC
HpaI 3324 GTTAAC GTT^AAC
HpaII 5415 CCGG C^CGG
KasI 5565 GGCGCC G^GCGCC
MamI 2979 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 5415 CCGG C^CGG
NarI 5565 GGCGCC GG^CGCC
NciI 5415 CCGGG CC^[GC]GG
NcoI 3571 CCATGG C^CATGG
NdeI 3536 CATATG CA^TATG
NheI 4689 GCTAGC G^CTAGC
PflMI 365 CCAGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 3593 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 4971 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3294 GAGCTC GAGCT^C
SfoI 5565 GGCGCC GGC^GCC
Van91I 365 CCAGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3888 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 1785 GTAGAC GT^[AC][GT]AC
2445 GTCTAC
AflII 889 CTTAAG C^TTAAG
3099 CTTAAG
AlwNI 1391 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
2192 CAGCAACTG
AocI 4238 CCTGAGG CC^T[ACGT]AGG
4954 CCTCAGG
BalI 3846 TGGCCA TGG^CCA
4624 TGGCCA
BanII 3294 GAGCTC G[GA]GC[CT]^C
4682 GAGCCC
BbsI 721 GAAGAC GAAGAC(2/6)
5104 GAAGAC
BclI 1813 TGATCA T^GATCA
4750 TGATCA
BfrI 889 CTTAAG C^TTAAG
3099 CTTAAG
BpuAI 721 GAAGAC GAAGAC(2/6)
5104 GAAGAC
BsmI 4644 GAATGC GAATGC(1/-1)
4328 GCATTC
BspLU11I 3985 ACATGT A^CATGT
4972 ACATGT
BspMI 3628 ACCTGC ACCTGC(4/8)
5258 GCAGGT
BstXI 1669 CCAGTACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5443 CCAGCACTTTGG
Bsu36I 4238 CCTGAGG CC^T[ACGT]AGG
4954 CCTCAGG
EaeI 3846 TGGCCA [CT]^GGCC[GA]
4624 TGGCCA
Eco81I 4238 CCTGAGG CC^T[ACGT]AGG
4954 CCTCAGG
EcoT22I 167 ATGCAT ATGCA^T
4646 ATGCAT
FbaI 1813 TGATCA T^GATCA
4750 TGATCA
HgaI 3508 GCGTC GACGC(5/10)
4756 GCGTC
MluNI 3846 TGGCCA TGG^CCA
4624 TGGCCA
MscI 3846 TGGCCA TGG^CCA
4624 TGGCCA
NsiI 167 ATGCAT ATGCA^T
4646 ATGCAT
SapI 508 GAAGAGC GCTCTTC(1/4)
4590 GAAGAGC
SpeI 347 ACTAGT A^CTAGT
1070 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI Asp700I
BbvCI BbvI BfaI BglII BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EarI EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PshBI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI