##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AspEI AviII
BbeI BbrPI BcgI BlpI Bpu1102I
BseAI BsiEI BsiWI BsmBI BspEI
BspMI BsrBI BssHII BstBI CelII
CpoI DraII DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
FseI FspI HaeII KasI KspI
MluI MroI NaeI NarI NgoMIV
NotI NruI NspV PinAI PmaCI
PmeI PmlI PpuMI PshAI PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 621 GACGTC GACGT^C
Acc65I 1898 GGTACC G^GTACC
AccII 492 CGCG CG^CG
AclI 3380 AACGTT AA^CGTT
AcyI 621 GACGTC G[GA]^CG[CT]C
AflIII 3897 ACATGT A^C[GA][CT]GT
AlwNI 5510 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 5871 CCTCAGG CC^T[ACGT]AGG
Asp718I 1898 GGTACC G^GTACC
AvrII 3953 CCTAGG C^CTAGG
BamHI 244 GGATCC G^GATCC
BbvCI 5755 CCTCAGC CCTCAGC(-5/-2)
BciVI 5076 GTATCC GTATCC(6/5)
BlnI 3953 CCTAGG C^CTAGG
BsaAI 1472 TACGTG [CT]AC^GT[GA]
BsaHI 621 GACGTC G[GA]^CG[CT]C
BsaWI 9 TCCGGT [AT]^CCGG[AT]
Bsp1407I 1285 TGTACA T^GTACA
BspLU11I 3897 ACATGT A^CATGT
BsrFI 2528 GCCGGT [GA]^CCGG[CT]
BsrGI 1285 TGTACA T^GTACA
BssSI 2259 CTCGTG CACGAG(-5/-1)
BstAPI 5720 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2249 GGTTACC G^GT[ACGT]ACC
BstPI 2249 GGTTACC G^GT[ACGT]ACC
BstUI 492 CGCG CG^CG
Bsu36I 5871 CCTCAGG CC^T[ACGT]AGG
CfoI 491 GCGC GCG^C
Cfr10I 2528 GCCGGT [GA]^CCGG[CT]
DsaI 2026 CCATGG C^C[GA][CT]GG
Eco81I 5871 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2249 GGTTACC G^GT[ACGT]ACC
EcoRV 2622 GATATC GAT^ATC
FauI 552 CCCGC CCCGC(4/6)
HgaI 788 GACGC GACGC(5/10)
HhaI 491 GCGC GCG^C
Hin1I 621 GACGTC G[GA]^CG[CT]C
HinP1I 491 GCGC G^CGC
HincII 4535 GTTAAC GT[CT]^[GA]AC
HindII 4535 GTTAAC GT[CT]^[GA]AC
HindIII 2046 AAGCTT A^AGCTT
HpaI 4535 GTTAAC GTT^AAC
KpnI 1898 GGTACC GGTAC^C
MvnI 492 CGCG CG^CG
NcoI 2026 CCATGG C^CATGG
NdeI 5010 CATATG CA^TATG
NheI 5970 GCTAGC G^CTAGC
PacI 2518 TTAATTAA TTAAT^TAA
Psp1406I 3380 AACGTT AA^CGTT
PvuII 660 CAGCTG CAG^CTG
SapI 1961 GAAGAGC GCTCTTC(1/4)
ScaI 967 AGTACT AGT^ACT
SexAI 2055 ACCAGGT A^CC[AT]GGT
SspBI 1285 TGTACA T^GTACA
StuI 3498 AGGCCT AGG^CCT
XbaI 2542 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspI 4634 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5255 GACCCTGTC
BanI 1898 GGTACC G^G[CT][GA]CC
5031 GGTGCC
BbsI 259 GAAGAC GAAGAC(2/6)
2396 GAAGAC
BpuAI 259 GAAGAC GAAGAC(2/6)
2396 GAAGAC
BspDI 2625 ATCGAT AT^CGAT
4126 ATCGAT
BspHI 2274 TCATGA T^CATGA
6048 TCATGA
ClaI 2625 ATCGAT AT^CGAT
4126 ATCGAT
DrdI 262 GACAGAATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2470 GACTTTAAAGTC
EcoRI 1164 GAATTC G^AATTC
5861 GAATTC
MfeI 504 CAATTG C^AATTG
605 CAATTG
MspA1I 144 CAGCGG C[AC]G^C[GT]G
660 CAGCTG
MunI 504 CAATTG C^AATTG
605 CAATTG
PaeR7I 2168 CTCGAG C^TCGAG
5739 CTCGAG
PflFI 4634 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5255 GACCCTGTC
PflMI 341 CCAATCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5022 CCAGGTAGTGG
PstI 1169 CTGCAG CTGCA^G
5516 CTGCAG
RcaI 2274 TCATGA T^CATGA
6048 TCATGA
SacI 2033 GAGCTC GAGCT^C
5216 GAGCTC
SpeI 926 ACTAGT A^CTAGT
964 ACTAGT
SspI 4902 AATATT AAT^ATT
5453 AATATT
Tth111I 4634 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5255 GACCCTGTC
Van91I 341 CCAATCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5022 CCAGGTAGTGG
XcmI 546 CCAGAGCCCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5929 CCATGCCCAGCCTGG
XhoI 2168 CTCGAG C^TCGAG
5739 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI ApoI AseI AsnI
Asp700I AspHI AvaI AvaII BalI
BanII BbvI BclI BcnI BfaI
BfrI BglI BglII BmyI BpmI
BsaBI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrDI BsrI
BssKI Bst1107I BstF5I BstNI BstXI
BstYI BstZ17I Cac8I Cfr13I DdeI
DpnII DraI EaeI EarI EcoRII
EcoT14I EcoT22I FbaI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PleI PshBI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI