##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AatII Acc65I AccIII AclI AhdI
AocI AscI Asp700I Asp718I AspEI
BbrPI BglII BseAI BspEI BssHII
Bst1107I BstZ17I Bsu36I CpoI DrdI
Eam1105I Eco81I EcoT22I FseI KpnI
MfeI MroI MunI NheI NotI
NruI NsiI PacI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SalI ScaI SgrAI SnaBI SpeI
SphI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AflII 5800 CTTAAG C^TTAAG
AgeI 2841 ACCGGT A^CCGGT
Aor51HI 3084 AGCGCT AGC^GCT
BamHI 4016 GGATCC G^GATCC
BfrI 5800 CTTAAG C^TTAAG
BlpI 2982 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2982 GCTCAGC GC^T[ACGT]AGC
BsaAI 602 TACGTG [CT]AC^GT[GA]
BsaBI 1412 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1371 CTCCTC GAGGAG(10/8)
BspDI 1607 ATCGAT AT^CGAT
BspMI 2928 ACCTGC ACCTGC(4/8)
BstEII 417 GGTCACC G^GT[ACGT]ACC
BstPI 417 GGTCACC G^GT[ACGT]ACC
CelII 2982 GCTCAGC GC^T[ACGT]AGC
ClaI 1607 ATCGAT AT^CGAT
Eco47III 3084 AGCGCT AGC^GCT
EcoNI 3791 CCTGTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 417 GGTCACC G^GT[ACGT]ACC
EcoRI 3891 GAATTC G^AATTC
EcoRV 2686 GATATC GAT^ATC
HpaI 4852 GTTAAC GTT^AAC
MamI 1412 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 3229 ACGCGT A^CGCGT
NaeI 2912 GCCGGC GCC^GGC
NcoI 4274 CCATGG C^CATGG
NdeI 5606 CATATG CA^TATG
NgoMIV 2912 GCCGGC G^CCGGC
PaeR7I 807 CTCGAG C^TCGAG
PinAI 2841 ACCGGT A^CCGGT
SbfI 3609 CCTGCAGG CCTGCA^GG
SfiI 3170 GGCCTTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 3609 CCTGCAGG CCTGCA^GG
XhoI 807 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 44 enzymes) #
###################################
AccI 1388 GTAGAC GT^[AC][GT]AC
3826 GTAGAC
AcyI 106 GGCGCC G[GA]^CG[CT]C
197 GGCGCC
Alw44I 3216 GTGCAC G^TGCAC
3642 GTGCAC
ApaLI 3216 GTGCAC G^TGCAC
3642 GTGCAC
AviII 2931 TGCGCA TGC^GCA
3580 TGCGCA
AvrII 4265 CCTAGG C^CTAGG
5120 CCTAGG
BalI 4780 TGGCCA TGG^CCA
5286 TGGCCA
BbeI 106 GGCGCC GGCGC^C
197 GGCGCC
BciVI 4495 GGATAC GTATCC(6/5)
5667 GGATAC
BclI 1830 TGATCA T^GATCA
3940 TGATCA
BlnI 4265 CCTAGG C^CTAGG
5120 CCTAGG
BsaHI 106 GGCGCC G[GA]^CG[CT]C
197 GGCGCC
BsaWI 2824 ACCGGA [AT]^CCGG[AT]
2841 ACCGGT
BsiEI 12 CGGCCG CG[GA][CT]^CG
34 CGGCCG
BsiWI 870 CGTACG C^GTACG
3075 CGTACG
BsmI 312 GAATGC GAATGC(1/-1)
1378 GCATTC
Bsp1407I 2220 TGTACA T^GTACA
3315 TGTACA
BspLU11I 2217 ACATGT A^CATGT
3903 ACATGT
BsrFI 2841 ACCGGT [GA]^CCGG[CT]
2912 GCCGGC
BsrGI 2220 TGTACA T^GTACA
3315 TGTACA
BssSI 154 CACGAG CACGAG(-5/-1)
3249 CTCGTG
Cfr10I 2841 ACCGGT [GA]^CCGG[CT]
2912 GCCGGC
EagI 12 CGGCCG C^GGCCG
34 CGGCCG
EclXI 12 CGGCCG C^GGCCG
34 CGGCCG
Eco52I 12 CGGCCG C^GGCCG
34 CGGCCG
FbaI 1830 TGATCA T^GATCA
3940 TGATCA
FspI 2931 TGCGCA TGC^GCA
3580 TGCGCA
Hin1I 106 GGCGCC G[GA]^CG[CT]C
197 GGCGCC
HindIII 547 AAGCTT A^AGCTT
2412 AAGCTT
KasI 106 GGCGCC G^GCGCC
197 GGCGCC
KspI 222 CCGCGG CCGC^GG
262 CCGCGG
MluNI 4780 TGGCCA TGG^CCA
5286 TGGCCA
MscI 4780 TGGCCA TGG^CCA
5286 TGGCCA
NarI 106 GGCGCC GG^CGCC
197 GGCGCC
PflMI 3183 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3298 CCAGGACCTGG
SacI 380 GAGCTC GAGCT^C
3061 GAGCTC
SacII 222 CCGCGG CCGC^GG
262 CCGCGG
SapI 1770 GAAGAGC GCTCTTC(1/4)
2777 GAAGAGC
SexAI 2022 ACCTGGT A^CC[AT]GGT
4301 ACCTGGT
SfoI 106 GGCGCC GGC^GCC
197 GGCGCC
SspBI 2220 TGTACA T^GTACA
3315 TGTACA
Van91I 3183 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3298 CCAGGACCTGG
XbaI 859 TCTAGA T^CTAGA
2560 TCTAGA
XcmI 1605 CCATCGATGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4003 CCATGTTGTGATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
AseI AsnI AspHI AspI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BmyI BpmI BpuAI BsaI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspHI
BsrBI BsrDI BsrI BssKI BstAPI
BstBI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
NspV PflFI PleI PpuMI PshBI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfuI
SmaI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XhoII
XmaCI XmaI