##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AocI Aor51HI ApaI AscI
Asp718I AviII BalI BamHI BanI
BbeI BsaHI BseAI BsiEI BsmBI
BspEI BspHI BspLU11I BsrBI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I Cfr10I CpoI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoO65I FseI FspI HaeII
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MluI MluNI
MroI MscI MvnI NaeI NarI
NgoMIV NotI NruI NspV PinAI
PmeI PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 671 GTCTAC GT^[AC][GT]AC
AclI 220 AACGTT AA^CGTT
AhdI 297 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1508 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 3045 ATTAAT AT^TAAT
AsnI 3045 ATTAAT AT^TAAT
AspEI 297 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3797 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 550 CCTAGG C^CTAGG
BbrPI 1698 CACGTG CAC^GTG
BcgI 1137 CGAGATACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 168 GTATCC GTATCC(6/5)
BglII 1740 AGATCT A^GATCT
BlnI 550 CCTAGG C^CTAGG
BlpI 3079 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 3079 GCTTAGC GC^T[ACGT]AGC
BsiWI 1013 CGTACG C^GTACG
Bsp1407I 2010 TGTACA T^GTACA
BspDI 2395 ATCGAT AT^CGAT
BsrGI 2010 TGTACA T^GTACA
BssSI 1135 CACGAG CACGAG(-5/-1)
BstAPI 1518 GCACTTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 3079 GCTTAGC GC^T[ACGT]AGC
CfoI 3849 GCGC GCG^C
ClaI 2395 ATCGAT AT^CGAT
DraIII 4004 CACGTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1737 GACAGATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 297 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 3689 GCGGG CCCGC(4/6)
HhaI 3849 GCGC GCG^C
HinP1I 3849 GCGC G^CGC
MfeI 896 CAATTG C^AATTG
MspA1I 3687 CAGCGG C[AC]G^C[GT]G
MunI 896 CAATTG C^AATTG
NcoI 3920 CCATGG C^CATGG
NdeI 5325 CATATG CA^TATG
NheI 4397 GCTAGC G^CTAGC
PacI 721 TTAATTAA TTAAT^TAA
PaeR7I 3894 CTCGAG C^TCGAG
PflFI 3797 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1698 CACGTG CAC^GTG
PmlI 1698 CACGTG CAC^GTG
PshAI 3488 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3045 ATTAAT AT^TAAT
Psp1406I 220 AACGTT AA^CGTT
SacI 4871 GAGCTC GAGCT^C
SexAI 3287 ACCAGGT A^CC[AT]GGT
SmaI 4860 CCCGGG CCC^GGG
SspBI 2010 TGTACA T^GTACA
SspI 4990 AATATT AAT^ATT
StuI 2580 AGGCCT AGG^CCT
Tth111I 3797 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3894 CTCGAG C^TCGAG
XmaCI 4860 CCCGGG C^CCGGG
XmaI 4860 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 1697 ACACGT A^C[GA][CT]GT
1699 ACGTGT
Asp700I 92 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5161 GAACTGTTTC
AvaI 3894 CTCGAG C^[CT]CG[GA]G
4860 CCCGGG
BbvCI 1463 CCTCAGC CCTCAGC(-5/-2)
3876 GCTGAGG
BclI 1491 TGATCA T^GATCA
4549 TGATCA
BglI 908 GCCCCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1504 GCCACAGGGGC
BsaAI 1698 CACGTG [CT]AC^GT[GA]
4004 CACGTA
BsaBI 515 GATCCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3161 GATGTTGATC
BsaWI 1067 TCCGGT [AT]^CCGG[AT]
3422 ACCGGA
BseRI 1461 CTCCTC GAGGAG(10/8)
3897 GAGGAG
BsoBI 3894 CTCGAG C^[CT]CG[GA]G
4860 CCCGGG
BspMI 1143 ACCTGC ACCTGC(4/8)
2748 ACCTGC
BsrDI 3579 CATTGC GCAATG(2/0)
2857 GCAATG
DsaI 524 CCGTGG C^C[GA][CT]GG
3920 CCATGG
EcoNI 822 CCTTTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5013 CCTAATAAAGG
EcoRI 601 GAATTC G^AATTC
5253 GAATTC
EcoRV 1361 GATATC GAT^ATC
2404 GATATC
FbaI 1491 TGATCA T^GATCA
4549 TGATCA
HindIII 1277 AAGCTT A^AGCTT
4273 AAGCTT
MamI 515 GATCCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3161 GATGTTGATC
PpuMI 1028 GGGACCT [GA]G^G[AT]CC[CT]
1157 GGGACCC
PstI 1108 CTGCAG CTGCA^G
1145 CTGCAG
ScaI 1636 AGTACT AGT^ACT
2689 AGTACT
SphI 972 GCATGC GCATG^C
3845 GCATGC
XbaI 306 TCTAGA T^CTAGA
2754 TCTAGA
XmnI 92 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5161 GAACTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
Alw44I AlwI ApaLI ApoI AspHI
AvaII BanII BbsI BbvI BcnI
BfaI BfrI BmyI BpmI BpuAI
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII