##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AflII AhdI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BciVI BfrI BlnI BsiWI
BsmI Bsp1407I BspDI BspLU11I BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI Eam1105I EcoRI
EcoT22I FseI FspI HincII HindII
HpaI KpnI MfeI MunI NheI
NotI NruI NsiI NspV PacI
PmeI PshAI PshBI PvuI RsrII
SalI ScaI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI SwaI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AclI 2016 AACGTT AA^CGTT
AgeI 2103 ACCGGT A^CCGGT
AspI 4210 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2273 GGATCC G^GATCC
BbrPI 518 CACGTG CAC^GTG
BclI 816 TGATCA T^GATCA
BglII 3231 AGATCT A^GATCT
BlpI 43 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 43 GCTGAGC GC^T[ACGT]AGC
BsaBI 817 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI 3976 GCAATG GCAATG(2/0)
CelII 43 GCTGAGC GC^T[ACGT]AGC
EcoRV 3085 GATATC GAT^ATC
FbaI 816 TGATCA T^GATCA
HindIII 3497 AAGCTT A^AGCTT
MamI 817 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 3962 ACGCGT A^CGCGT
NcoI 1692 CCATGG C^CATGG
NdeI 5278 CATATG CA^TATG
NspI 1284 GCATGT [GA]CATG^[CT]
PaeR7I 2482 CTCGAG C^TCGAG
PflFI 4210 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 880 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2103 ACCGGT A^CCGGT
PmaCI 518 CACGTG CAC^GTG
PmlI 518 CACGTG CAC^GTG
Psp1406I 2016 AACGTT AA^CGTT
SapI 3070 GAAGAGC GCTCTTC(1/4)
SbfI 2402 CCTGCAGG CCTGCA^GG
SfiI 2399 GGCCCTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 2402 CCTGCAGG CCTGCA^GG
Tth111I 4210 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 880 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2482 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 2921 GACGTC GACGT^C
3050 GACGTC
AccIII 804 TCCGGA T^CCGGA
3278 TCCGGA
AocI 866 CCTGAGG CC^T[ACGT]AGG
5155 CCTCAGG
Aor51HI 1170 AGCGCT AGC^GCT
5457 AGCGCT
BbsI 2183 GAAGAC GAAGAC(2/6)
2423 GTCTTC
BpuAI 2183 GAAGAC GAAGAC(2/6)
2423 GTCTTC
BseAI 804 TCCGGA T^CCGGA
3278 TCCGGA
BsiEI 427 CGGCCG CG[GA][CT]^CG
2578 CGGCCG
BspEI 804 TCCGGA T^CCGGA
3278 TCCGGA
BssSI 3720 CTCGTG CACGAG(-5/-1)
3740 CTCGTG
BstEII 432 GGTCACC G^GT[ACGT]ACC
4737 GGTCACC
BstPI 432 GGTCACC G^GT[ACGT]ACC
4737 GGTCACC
Bsu36I 866 CCTGAGG CC^T[ACGT]AGG
5155 CCTCAGG
EagI 427 CGGCCG C^GGCCG
2578 CGGCCG
EclXI 427 CGGCCG C^GGCCG
2578 CGGCCG
Eco47III 1170 AGCGCT AGC^GCT
5457 AGCGCT
Eco52I 427 CGGCCG C^GGCCG
2578 CGGCCG
Eco81I 866 CCTGAGG CC^T[ACGT]AGG
5155 CCTCAGG
EcoO65I 432 GGTCACC G^GT[ACGT]ACC
4737 GGTCACC
KspI 25 CCGCGG CCGC^GG
3644 CCGCGG
MroI 804 TCCGGA T^CCGGA
3278 TCCGGA
NaeI 2229 GCCGGC GCC^GGC
2576 GCCGGC
NgoMIV 2229 GCCGGC G^CCGGC
2576 GCCGGC
SacI 3424 GAGCTC GAGCT^C
3737 GAGCTC
SacII 25 CCGCGG CCGC^GG
3644 CCGCGG
StuI 2564 AGGCCT AGG^CCT
5032 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI ApaI ApaLI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvCI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI