##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AgeI AhdI Aor51HI ApaI
AscI AspEI AviII AvrII BbeI
BbrPI BfrI BglI BglII BlnI
BsaAI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI Bsp1407I BspDI BspEI
BsrBI BsrGI BssHII Bst1107I BstEII
BstPI BstUI BstZ17I ClaI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV FauI FseI
FspI HaeII Hin1I HpaI KasI
KspI MaeII MluI MroI MvnI
NaeI NarI NgoMIV NotI NruI
PacI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SnaBI Sse8387I SspBI
StuI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 4565 GGTACC G^GTACC
AccI 1333 GTAGAC GT^[AC][GT]AC
Alw44I 4023 GTGCAC G^TGCAC
ApaLI 4023 GTGCAC G^TGCAC
Asp718I 4565 GGTACC G^GTACC
AspI 2200 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 4565 GGTACC G^G[CT][GA]CC
BanII 744 GAGCCC G[GA]GC[CT]^C
BcgI 1849 CGACCAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsmBI 1065 CGTCTC CGTCTC(1/5)
BsrFI 1090 GCCGGT [GA]^CCGG[CT]
BstAPI 3567 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 3941 GCGC GCG^C
Cfr10I 1090 GCCGGT [GA]^CCGG[CT]
HhaI 3941 GCGC GCG^C
HinP1I 3941 GCGC G^CGC
KpnI 4565 GGTACC GGTAC^C
NheI 4958 GCTAGC G^CTAGC
PflFI 2200 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 1963 CAGCTG CAG^CTG
ScaI 499 AGTACT AGT^ACT
SmaI 2402 CCCGGG CCC^GGG
SpeI 464 ACTAGT A^CTAGT
SphI 5285 GCATGC GCATG^C
SwaI 4720 ATTTAAAT ATTT^AAAT
Tth111I 2200 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2402 CCCGGG C^CCGGG
XmaI 2402 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 49 enzymes) #
###################################
AflIII 331 ACATGT A^C[GA][CT]GT
3107 ACATGT
AocI 18 CCTGAGG CC^T[ACGT]AGG
4964 CCTCAGG
AseI 3778 ATTAAT AT^TAAT
4284 ATTAAT
AsnI 3778 ATTAAT AT^TAAT
4284 ATTAAT
Asp700I 768 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2538 GAAGAAGTTC
BalI 1934 TGGCCA TGG^CCA
1951 TGGCCA
BamHI 1181 GGATCC G^GATCC
2805 GGATCC
BbvCI 3645 CCTCAGC CCTCAGC(-5/-2)
315 GCTGAGG
BciVI 4783 GGATAC GTATCC(6/5)
3299 GTATCC
BclI 947 TGATCA T^GATCA
3329 TGATCA
BcnI 2402 CCCGG CC^[GC]GG
2403 CCGGG
BlpI 219 GCTAAGC GC^T[ACGT]AGC
1648 GCTCAGC
Bpu1102I 219 GCTAAGC GC^T[ACGT]AGC
1648 GCTCAGC
BsaBI 942 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3220 GATTTTCATC
BsaI 1462 GAGACC GGTCTC(1/5)
1093 GGTCTC
BseRI 21 GAGGAG GAGGAG(10/8)
4330 GAGGAG
BspHI 1037 TCATGA T^CATGA
2645 TCATGA
BspLU11I 331 ACATGT A^CATGT
3107 ACATGT
BspMI 1693 GCAGGT ACCTGC(4/8)
3434 GCAGGT
BssSI 106 CACGAG CACGAG(-5/-1)
5545 CTCGTG
BstBI 172 TTCGAA TT^CGAA
1072 TTCGAA
BstXI 94 CCAACACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4347 CCATTCTGTTGG
Bsu36I 18 CCTGAGG CC^T[ACGT]AGG
4964 CCTCAGG
CelII 219 GCTAAGC GC^T[ACGT]AGC
1648 GCTCAGC
DrdI 1085 GACACGCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2245 GACCACTCAGTC
EaeI 1934 TGGCCA [CT]^GGCC[GA]
1951 TGGCCA
Eco81I 18 CCTGAGG CC^T[ACGT]AGG
4964 CCTCAGG
EcoT22I 1822 ATGCAT ATGCA^T
5283 ATGCAT
FbaI 947 TGATCA T^GATCA
3329 TGATCA
HincII 2254 GTCAAC GT[CT]^[GA]AC
4117 GTCAAC
HindII 2254 GTCAAC GT[CT]^[GA]AC
4117 GTCAAC
HindIII 1197 AAGCTT A^AGCTT
2475 AAGCTT
MamI 942 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3220 GATTTTCATC
MluNI 1934 TGGCCA TGG^CCA
1951 TGGCCA
MscI 1934 TGGCCA TGG^CCA
1951 TGGCCA
NciI 2402 CCCGG CC^[GC]GG
2403 CCGGG
NcoI 3166 CCATGG C^CATGG
3401 CCATGG
NdeI 2533 CATATG CA^TATG
4301 CATATG
NsiI 1822 ATGCAT ATGCA^T
5283 ATGCAT
NspV 172 TTCGAA TT^CGAA
1072 TTCGAA
PaeR7I 2065 CTCGAG C^TCGAG
2995 CTCGAG
PpuMI 2789 AGGTCCT [GA]G^G[AT]CC[CT]
2838 GGGTCCC
PshBI 3778 ATTAAT AT^TAAT
4284 ATTAAT
RcaI 1037 TCATGA T^CATGA
2645 TCATGA
SapI 513 GCTCTTC GCTCTTC(1/4)
1765 GCTCTTC
SfuI 172 TTCGAA TT^CGAA
1072 TTCGAA
XcmI 3458 CCATAGTAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5375 CCAGTTGGAATGTGG
XhoI 2065 CTCGAG C^TCGAG
2995 CTCGAG
XmnI 768 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2538 GAAGAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoNI
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MfeI MflI
MnlI MseI MslI MspA1I MspI
MunI MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XhoII