##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AgeI AhdI AocI Aor51HI
ApaI AscI Asp718I AspEI AviII
BbrPI BbsI BclI BpuAI BsaI
BsiWI BspDI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I FbaI
FseI FspI KpnI KspI MluI
NdeI NheI NotI NruI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SacII SbfI
SpeI Sse8387I Van91I XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 652 GACGTC GACGT^C
AccIII 2699 TCCGGA T^CCGGA
AclI 1144 AACGTT AA^CGTT
AflII 5934 CTTAAG C^TTAAG
AseI 1939 ATTAAT AT^TAAT
AsnI 1939 ATTAAT AT^TAAT
Asp700I 669 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2590 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 813 CCTAGG C^CTAGG
BfrI 5934 CTTAAG C^TTAAG
BglI 1694 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 813 CCTAGG C^CTAGG
BlpI 1782 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1782 GCTTAGC GC^T[ACGT]AGC
BsaAI 4878 TACGTA [CT]AC^GT[GA]
BsaBI 4704 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2699 TCCGGA T^CCGGA
BsiEI 222 CGACCG CG[GA][CT]^CG
BsmBI 654 CGTCTC CGTCTC(1/5)
BspEI 2699 TCCGGA T^CCGGA
BsrBI 236 CCGCTC CCGCTC(-3/-3)
BstBI 1531 TTCGAA TT^CGAA
BstXI 3990 CCAGGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1782 GCTTAGC GC^T[ACGT]AGC
DraIII 2068 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2057 GACAACACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 313 GAAGAG CTCTTC(1/4)
HindIII 1780 AAGCTT A^AGCTT
Ksp632I 313 GAAGAG CTCTTC(1/4)
MamI 4704 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2699 TCCGGA T^CCGGA
NspV 1531 TTCGAA TT^CGAA
PacI 3733 TTAATTAA TTAAT^TAA
PflFI 2590 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1825 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 1939 ATTAAT AT^TAAT
Psp1406I 1144 AACGTT AA^CGTT
SacI 346 GAGCTC GAGCT^C
SalI 587 GTCGAC G^TCGAC
SapI 313 GAAGAGC GCTCTTC(1/4)
ScaI 3273 AGTACT AGT^ACT
SexAI 2169 ACCAGGT A^CC[AT]GGT
SfiI 1693 GGCCATGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1531 TTCGAA TT^CGAA
SgrAI 298 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 278 CCCGGG CCC^GGG
SnaBI 4878 TACGTA TAC^GTA
SphI 3023 GCATGC GCATG^C
SwaI 5481 ATTTAAAT ATTT^AAAT
Tth111I 2590 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 278 CCCGGG C^CCGGG
XmaI 278 CCCGGG C^CCGGG
XmnI 669 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 587 GTCGAC GT^[AC][GT]AC
2919 GTAGAC
Alw44I 2384 GTGCAC G^TGCAC
2982 GTGCAC
ApaLI 2384 GTGCAC G^TGCAC
2982 GTGCAC
AvaI 278 CCCGGG C^[CT]CG[GA]G
2103 CTCGGG
AvaII 1826 GGACC G^G[AT]CC
1846 GGTCC
BalI 1692 TGGCCA TGG^CCA
3338 TGGCCA
BamHI 167 GGATCC G^GATCC
5557 GGATCC
BbvCI 2643 CCTCAGC CCTCAGC(-5/-2)
2757 GCTGAGG
BcgI 1044 CGAAGGGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1523 GCACATCCTTCG
BciVI 4745 GTATCC GTATCC(6/5)
5680 GTATCC
BseRI 991 GAGGAG GAGGAG(10/8)
1998 GAGGAG
BsmI 2818 GCATTC GAATGC(1/-1)
3869 GCATTC
BsoBI 278 CCCGGG C^[CT]CG[GA]G
2103 CTCGGG
BsrFI 4 GCCGGC [GA]^CCGG[CT]
299 GCCGGC
Cfr10I 4 GCCGGC [GA]^CCGG[CT]
299 GCCGGC
EcoRI 2223 GAATTC G^AATTC
3902 GAATTC
EcoRV 858 GATATC GAT^ATC
4579 GATATC
HgaI 1662 GACGC GACGC(5/10)
91 GCGTC
HpaI 2673 GTTAAC GTT^AAC
4471 GTTAAC
MluNI 1692 TGGCCA TGG^CCA
3338 TGGCCA
MscI 1692 TGGCCA TGG^CCA
3338 TGGCCA
NaeI 4 GCCGGC GCC^GGC
299 GCCGGC
NgoMIV 4 GCCGGC G^CCGGC
299 GCCGGC
StuI 2258 AGGCCT AGG^CCT
4800 AGGCCT
XbaI 677 TCTAGA T^CTAGA
4706 TCTAGA
XcmI 1083 CCAAATTGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1543 CCAAGTTGAAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI BanI BanII BbeI BbvI
BcnI BfaI BglII BmyI BpmI
BsaHI BsaJI BsaWI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspHI BspLU11I BspMI BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MspI MunI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PleI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII